FastQCFastQC Report
Mon 15 Aug 2016
SRR2920842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813997
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT402.7590903E-416.6256
CTGTTTA759.656414E-713.9333349
GTAGGAC751.4765355E-512.6666673
GGATAAC658.016223E-411.6923071
GTCTAAG752.0715022E-411.4000011
TTACACC752.0715022E-411.4000014
CTTACAC1251.8259016E-811.4000013
GAGTGTC600.00587158611.0833337
CTTATCC600.00587158611.0833333
GATATAA600.00587158611.0833331
CTCCTAG600.00587158611.0833331
AGCACCG803.7665176E-410.68755
GTTACAC1106.032622E-610.3636363
ACGCAGA750.00264525510.1333341
GTCTGTT856.58576E-410.0588247
GTCCTAC856.58576E-410.0588241
TAGACAG1356.4235974E-79.8518525
GTTTAAG800.004511559.51
CTATACA900.00111231429.54
GGACTGT1304.2275897E-69.56
Produced by FastQC (version 0.11.5)