##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920842_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	813997
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64043970678025	33.0	32.0	34.0	32.0	34.0
2	32.85254368259343	33.0	32.0	34.0	32.0	34.0
3	33.061221355852666	33.0	33.0	34.0	32.0	34.0
4	33.10218588029194	33.0	33.0	34.0	32.0	34.0
5	33.017739623119006	33.0	33.0	34.0	32.0	34.0
6	35.81359145058274	37.0	37.0	37.0	33.0	37.0
7	35.863583035318314	37.0	37.0	37.0	33.0	37.0
8	35.80041941186516	37.0	36.0	37.0	33.0	37.0
9	35.79079775478288	37.0	35.0	37.0	33.0	37.0
10	35.73993393096043	37.0	35.0	37.0	33.0	37.0
11	35.664294831553434	37.0	34.0	37.0	33.0	37.0
12	35.669180598945694	37.0	34.0	37.0	33.0	37.0
13	37.37049522295537	38.0	38.0	38.0	37.0	38.0
14	37.37928763865223	38.0	38.0	38.0	37.0	38.0
15	37.36021385828203	38.0	38.0	38.0	37.0	38.0
16	37.33394594820374	38.0	38.0	38.0	37.0	38.0
17	37.36190919622554	38.0	38.0	38.0	37.0	38.0
18	37.41501258604147	38.0	38.0	38.0	37.0	38.0
19	37.41550030282667	38.0	38.0	38.0	37.0	38.0
20	37.34833666463144	38.0	38.0	38.0	37.0	38.0
21	37.30701095949985	38.0	38.0	38.0	37.0	38.0
22	37.37523356965689	38.0	38.0	38.0	37.0	38.0
23	38.10167113638011	39.0	38.0	39.0	37.0	39.0
24	38.02142882590476	39.0	38.0	39.0	37.0	39.0
25	37.954220961502315	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	4.0
22	11.0
23	48.0
24	136.0
25	299.0
26	693.0
27	1377.0
28	2475.0
29	3965.0
30	6298.0
31	9156.0
32	13594.0
33	20753.0
34	36386.0
35	102303.0
36	494537.0
37	121962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.386035820770836	17.497116082737403	12.295131308837748	25.821716787654008
2	16.825983388145165	19.411373751991714	37.76610970310701	25.996533156756108
3	20.540001990179324	21.75511703360086	27.997031930093108	29.707849046126704
4	14.013688011135175	15.345019699089802	35.406395846667735	35.23489644310728
5	15.416273032947295	35.35406150145516	34.394967057618146	14.83469840797939
6	35.14374131599994	34.385630413871304	16.524139523855737	13.946488746273019
7	30.475050890850948	29.558094194450348	20.37869918439503	19.588155730303676
8	26.57601932193853	34.67187225505745	19.532013017246992	19.220095405757025
9	25.434737474462437	16.48716150059521	19.119112232600365	38.958988792341984
10	15.242808020176978	27.526145673755554	32.74250396500233	24.488542341065138
11	36.05246702383424	22.170720530911048	22.950944536650628	18.825867908604085
12	24.220113833343365	23.200822607454327	29.512270929745444	23.066792629456863
13	28.726887199829974	20.499338449650306	25.51348469343253	25.26028965708719
14	22.33558600338822	19.95781311233334	26.027122950084582	31.679477934193862
15	24.018761739908133	28.202806644250533	22.43644632596926	25.341985289872078
16	24.698371124217903	27.264719648843915	24.0943148439122	23.94259438302598
17	23.512371667217447	27.01164746307419	26.063732421618262	23.412248448090104
18	23.719620588282268	26.818526358205254	26.27982658412746	23.182026469385022
19	24.643702618068616	26.267418675990207	25.84088147745016	23.247997228491013
20	24.234978752992948	26.352554124892354	25.937933432187098	23.474533689927604
21	24.163602568559835	25.869751362720013	25.91815448951286	24.048491579207294
22	24.159917051291345	25.754517522791854	25.93252800685998	24.153037419056826
23	24.042717602153324	25.68989812001764	26.00169288093199	24.26569139689704
24	23.90057948616518	25.968277524364343	25.999235869419667	24.131907120050812
25	24.220359534494598	25.81152018987785	25.958326627739414	24.009793647888138
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	9.0
3	16.0
4	16.0
5	16.0
6	62.5
7	109.0
8	109.0
9	109.0
10	346.5
11	584.0
12	584.0
13	584.0
14	1065.5
15	1547.0
16	1547.0
17	1547.0
18	3065.0
19	4583.0
20	4583.0
21	4583.0
22	7797.5
23	11012.0
24	11012.0
25	11012.0
26	16411.0
27	21810.0
28	21810.0
29	21810.0
30	29801.5
31	37793.0
32	37793.0
33	37793.0
34	46769.0
35	55745.0
36	55745.0
37	55745.0
38	66811.5
39	77878.0
40	77878.0
41	77878.0
42	87363.0
43	96848.0
44	96848.0
45	96848.0
46	103875.5
47	110903.0
48	110903.0
49	110903.0
50	111343.0
51	111783.0
52	111783.0
53	111783.0
54	106380.5
55	100978.0
56	100978.0
57	100978.0
58	89855.0
59	78732.0
60	78732.0
61	78732.0
62	65886.5
63	53041.0
64	53041.0
65	53041.0
66	41446.0
67	29851.0
68	29851.0
69	29851.0
70	21831.0
71	13811.0
72	13811.0
73	13811.0
74	9362.5
75	4914.0
76	4914.0
77	4914.0
78	3211.0
79	1508.0
80	1508.0
81	1508.0
82	962.5
83	417.0
84	417.0
85	417.0
86	266.5
87	116.0
88	116.0
89	116.0
90	65.5
91	15.0
92	15.0
93	15.0
94	8.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	813997.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.48022705431327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.13009850323598	84.2278258671443
2	4.032130936093961	7.215919833488167
3	0.9164093145698216	2.4600154062718578
4	0.35348797925612413	1.265207385792335
5	0.18013430669485786	0.8059229331663593
6	0.10448329018685973	0.5609513117581147
7	0.06693470933703523	0.4192533092504663
8	0.04624739007541531	0.33105815716940457
9	0.03217503625958734	0.25911265949897744
>10	0.13102226428694722	1.941855466105336
>50	0.005566583696939363	0.31702216487796364
>100	0.001309686306502167	0.19585550547669786
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2285057561637206E-4
2	0.0	0.0	0.0	0.0	1.2285057561637206E-4
3	0.0	0.0	0.0	0.0	1.2285057561637206E-4
4	0.0	0.0	0.0	0.0	1.2285057561637206E-4
5	0.0	0.0	0.0	0.0	1.2285057561637206E-4
6	0.0	0.0	0.0	0.0	1.2285057561637206E-4
7	0.0	0.0	0.0	0.0	1.2285057561637206E-4
8	0.0	0.0	0.0	0.0	1.2285057561637206E-4
9	0.0	0.0	0.0	1.2285057561637206E-4	1.2285057561637206E-4
10	0.0	0.0	0.0	1.2285057561637206E-4	2.457011512327441E-4
11	0.0	0.0	0.0	1.2285057561637206E-4	2.457011512327441E-4
12	0.0	0.0	0.0	1.2285057561637206E-4	3.6855172684911616E-4
13	0.0	0.0	0.0	1.2285057561637206E-4	4.914023024654883E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	40	2.7590903E-4	16.625	6
CTGTTTA	75	9.656414E-7	13.933334	9
GTAGGAC	75	1.4765355E-5	12.666667	3
GGATAAC	65	8.016223E-4	11.692307	1
GTCTAAG	75	2.0715022E-4	11.400001	1
TTACACC	75	2.0715022E-4	11.400001	4
CTTACAC	125	1.8259016E-8	11.400001	3
GAGTGTC	60	0.005871586	11.083333	7
CTTATCC	60	0.005871586	11.083333	3
GATATAA	60	0.005871586	11.083333	1
CTCCTAG	60	0.005871586	11.083333	1
AGCACCG	80	3.7665176E-4	10.6875	5
GTTACAC	110	6.032622E-6	10.363636	3
ACGCAGA	75	0.002645255	10.133334	1
GTCTGTT	85	6.58576E-4	10.058824	7
GTCCTAC	85	6.58576E-4	10.058824	1
TAGACAG	135	6.4235974E-7	9.851852	5
GTTTAAG	80	0.00451155	9.5	1
CTATACA	90	0.0011123142	9.5	4
GGACTGT	130	4.2275897E-6	9.5	6
>>END_MODULE