FastQCFastQC Report
Mon 15 Aug 2016
SRR2920841_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920841_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437268
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCC400.00527486814.2484573
TTCCTAA400.00527486814.2484572
TCTAGGG500.001497765813.298563
CAGGACT801.99247E-613.0610864
CTAAGAC957.349627E-812.9985913
ACAGAGC957.349627E-812.9985918
GTGTAAG604.014889E-412.6914181
GATATAC701.06779975E-412.23815251
TATACAG752.069405E-411.3987665
GTCCTAA1850.011.3193721
AGAACTG1104.943322E-711.2260575
GTAGGAC3400.011.175263
ATGGCAA2300.011.15096617
GTATTAG600.00577959311.104991
CCCTAAG600.00577959311.104991
GTGCTAA600.00577959311.104991
GTACTGC600.005868952311.0821336
ACCAACC600.005868952311.0821338
ATTATAC600.005868952311.0821333
TAGGACA1201.2676901E-711.0821334
Produced by FastQC (version 0.11.5)