##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920841_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	437268
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.30189952157487	32.0	32.0	33.0	18.0	33.0
2	30.512568950849364	32.0	32.0	33.0	18.0	33.0
3	30.755708169818053	32.0	32.0	33.0	29.0	34.0
4	30.638130391430426	32.0	32.0	33.0	18.0	34.0
5	30.751003960957583	32.0	32.0	33.0	29.0	34.0
6	33.5138473430482	37.0	33.0	37.0	19.0	37.0
7	33.89604087195953	37.0	33.0	37.0	29.0	37.0
8	33.56304829075075	37.0	33.0	37.0	19.0	37.0
9	33.930161822955256	37.0	33.0	37.0	29.0	37.0
10	33.81020335354977	37.0	33.0	37.0	29.0	37.0
11	33.78471097816442	37.0	33.0	37.0	29.0	37.0
12	33.846414555833036	37.0	33.0	37.0	29.0	37.0
13	35.10382419934685	38.0	36.0	38.0	30.0	38.0
14	34.88939048821318	38.0	36.0	38.0	19.0	38.0
15	35.08697412113395	38.0	36.0	38.0	30.0	38.0
16	35.137437452546266	38.0	36.0	38.0	32.0	38.0
17	34.98943439721178	38.0	36.0	38.0	20.0	38.0
18	34.92049955633616	38.0	36.0	38.0	20.0	38.0
19	35.08230650310565	38.0	36.0	38.0	30.0	38.0
20	35.00312165536925	38.0	36.0	38.0	20.0	38.0
21	34.938769816222546	38.0	36.0	38.0	20.0	38.0
22	34.84999130967736	38.0	36.0	38.0	19.0	38.0
23	35.32729584602578	38.0	36.0	39.0	20.0	39.0
24	35.25998243640056	38.0	36.0	39.0	19.0	39.0
25	34.39909163259146	38.0	34.0	39.0	18.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	6.0
17	12.0
18	55.0
19	245.0
20	657.0
21	1560.0
22	2817.0
23	4565.0
24	6340.0
25	7736.0
26	9351.0
27	10854.0
28	12476.0
29	14015.0
30	15575.0
31	17780.0
32	21434.0
33	27468.0
34	41191.0
35	79644.0
36	155050.0
37	8436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.94255781340226	18.926041007873437	13.284937809919247	25.84646336880505
2	14.927950199763531	19.199710931764802	41.0970871344053	24.775251734066373
3	18.657207936551497	23.226716796106736	29.402791880494345	28.713283386847426
4	12.44820110321359	15.95291674670911	37.58358718223149	34.015294967845804
5	13.835908413147086	36.57962622464942	36.03899667938198	13.545468682821518
6	32.941811429146426	36.34887528929627	17.429585517348627	13.279727764208676
7	28.44662769743041	30.558833484270515	22.426749727855686	18.56778909044339
8	24.736373885978132	37.42061486460217	20.33791710785401	17.50509414156568
9	25.217029373290522	16.09356275785102	20.539348866141587	38.150059002716866
10	14.4229625767264	28.68675503352635	34.57307646569152	22.317205924055727
11	34.736591746937805	22.442072138825615	24.44610627807203	18.37522983616455
12	22.9186677278008	23.82772121444972	31.72699580120201	21.526615256547473
13	28.25040935993487	21.437653795841452	26.20360968559328	24.108327158630406
14	20.736481974441304	21.30066686791624	27.181270982555322	30.781580175087132
15	22.830163652496868	30.65351226250263	22.930102362852985	23.586221722147517
16	22.789685044412124	27.830986946220627	26.80667233824565	22.5726556711216
17	21.94626636296276	28.361096627240045	27.689197471573497	22.0034395382237
18	22.27466908166159	27.449756213580688	28.757649770849913	21.51792493390781
19	23.669694558028485	26.97842055672951	26.559455528417352	22.79242935682465
20	23.272912721717574	27.341813258688035	27.373830236834163	22.011443782760228
21	22.812554314516497	26.806443645544608	26.576150095593547	23.804851944345344
22	23.095904571109706	27.542376757503405	26.80964534335922	22.552073328027664
23	22.53423529734625	27.24004500672357	27.24713448045592	22.97858521547426
24	23.362102875124638	26.894719028147495	27.368570304710154	22.37460779201771
25	23.284576049470804	27.027589487453916	27.161603410265556	22.52623105280972
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	3.0
2	12.5
3	22.0
4	22.0
5	22.0
6	80.5
7	139.0
8	139.0
9	139.0
10	367.0
11	595.0
12	595.0
13	595.0
14	1270.0
15	1945.0
16	1945.0
17	1945.0
18	3450.5
19	4956.0
20	4956.0
21	4956.0
22	7787.5
23	10619.0
24	10619.0
25	10619.0
26	14989.0
27	19359.0
28	19359.0
29	19359.0
30	24921.0
31	30483.0
32	30483.0
33	30483.0
34	35396.5
35	40310.0
36	40310.0
37	40310.0
38	44059.0
39	47808.0
40	47808.0
41	47808.0
42	50498.5
43	53189.0
44	53189.0
45	53189.0
46	54068.5
47	54948.0
48	54948.0
49	54948.0
50	52971.5
51	50995.0
52	50995.0
53	50995.0
54	46879.0
55	42763.0
56	42763.0
57	42763.0
58	37954.5
59	33146.0
60	33146.0
61	33146.0
62	27698.5
63	22251.0
64	22251.0
65	22251.0
66	17612.5
67	12974.0
68	12974.0
69	12974.0
70	9670.5
71	6367.0
72	6367.0
73	6367.0
74	4486.0
75	2605.0
76	2605.0
77	2605.0
78	1861.5
79	1118.0
80	1118.0
81	1118.0
82	770.0
83	422.0
84	422.0
85	422.0
86	303.5
87	185.0
88	185.0
89	185.0
90	124.5
91	64.0
92	64.0
93	64.0
94	33.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.197590493701803
2	2.286927010437535E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	2.286927010437535E-4
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	437268.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.24168088015522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.76990422406773	92.16528336911841
2	2.4884042230988603	4.739996018344245
3	0.40331425335948634	1.152369822386468
4	0.1550162895866397	0.5905604793614587
5	0.06632491016833408	0.31584479643287183
6	0.038476417815227204	0.21987352241816327
7	0.017930826321877372	0.11954334269260122
8	0.014983533320057774	0.11416455191408921
9	0.010166801896990072	0.08714729716603965
>10	0.0352255732756573	0.4757030043525879
>50	2.5294708912546406E-4	0.019513795813061816
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	6.860781031312605E-4
13	0.0	0.0	0.0	0.0	6.860781031312605E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCC	40	0.005274868	14.248457	3
TTCCTAA	40	0.005274868	14.248457	2
TCTAGGG	50	0.0014977658	13.29856	3
CAGGACT	80	1.99247E-6	13.061086	4
CTAAGAC	95	7.349627E-8	12.998591	3
ACAGAGC	95	7.349627E-8	12.998591	8
GTGTAAG	60	4.014889E-4	12.691418	1
GATATAC	70	1.06779975E-4	12.2381525	1
TATACAG	75	2.069405E-4	11.398766	5
GTCCTAA	185	0.0	11.319372	1
AGAACTG	110	4.943322E-7	11.226057	5
GTAGGAC	340	0.0	11.17526	3
ATGGCAA	230	0.0	11.150966	17
GTATTAG	60	0.005779593	11.10499	1
CCCTAAG	60	0.005779593	11.10499	1
GTGCTAA	60	0.005779593	11.10499	1
GTACTGC	60	0.0058689523	11.082133	6
ACCAACC	60	0.0058689523	11.082133	8
ATTATAC	60	0.0058689523	11.082133	3
TAGGACA	120	1.2676901E-7	11.082133	4
>>END_MODULE