FastQCFastQC Report
Mon 15 Aug 2016
SRR2920841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437268
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCCT400.005265184714.2524014
AATCCCG759.645992E-713.93090219
GTGTAGG707.1534378E-613.5861491
TCAGGAC707.2214425E-613.5737163
AGCCGCC500.001498513613.29767917
TAAAAGA801.9862819E-613.0647024
TCTATAC604.0757784E-412.6688023
TATACAC1800.012.1409353
TCAGACC550.003058520412.0929473
GGCGAGG1552.5465852E-1111.64312819
TACACTG2050.011.5873195
AGGACGT3300.011.2291665
CAGGACC1104.9256596E-711.2291654
AACAGTG1104.932954E-711.22788057
CGCAGAG855.2870993E-511.1796342
GATATAC1451.1823431E-911.1500121
GTCCTAC3000.011.0953551
AGGACCC600.00585686211.0852025
GTAGTAG600.00585686211.0852023
ACTGTGC1201.268836E-711.0813998
Produced by FastQC (version 0.11.5)