FastQCFastQC Report
Mon 15 Aug 2016
SRR2920840_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920840_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523292
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTATTCCATTATTCCTAGCTGC26120.17147073574862862No Hit
GAATAACGCCGCCGCATCGCCAGTC25780.1692387277028961No Hit
GTATCTGATCGTCTTCGAACCTCCG25760.16910743311197066No Hit
CTATTGGAGCTGGAATTACCGCGGC25580.16792578179364168No Hit
GAATAGGACCGCGGTTCTATTTTGT23650.1552558537693364No Hit
GAACTACGACGGTATCTGATCGTCT23570.15473067540563465No Hit
GTCTTGCGCCGGTCCAAGAATTTCA22420.14718123642742165No Hit
CTTTAATATACGCTATTGGAGCTGG22390.14698429454103348No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG22300.14639346888186902No Hit
CTCTTAATCATGGCCTCAGTTCCGA21070.13831885153995424No Hit
GATTAAGAGGGACGGCCGGGGGCAT19800.1299816450161886No Hit
GTACATGGGTACCTGGTTGATCCTG19340.12696186942490342No Hit
GTCCTGTATTGTTATTTTTCGTCAC19250.12637104376573893No Hit
ATACAGGACTCTTTCGAGGCCCTGT19220.12617410187935077No Hit
ATATTAAAGTTGCTGCAGTTAAAAA18940.12433597760639456No Hit
GCTTTGAACACTCTAATTTTTTCAA18700.12276044251528925No Hit
GTACATGGGAATAACGCCGCCGCAT18450.1211192601287212No Hit
CTCTAGATAACCTCGGGCCGATCGC18150.11914984126483957No Hit
GTATCAACGCAGAGTACATGGGAAT16700.10963098342274494No Hit
CCATTATTCCTAGCTGCGGTATCCA16080.1055608511040562No Hit
GAATAATGGAATAGGACCGCGGTTC15700.10306625387647281No Hit
GCTAAGAGCATCGAGGGGGCGCCGA15630.10260672280823374No Hit
GTATTGTTATTTTTCGTCACTACCT15470.10155636608083021No Hit
CTTTAAATCCTTTAACGAGGATCCA15350.10076859853527755No Hit
AGATAACCTCGGGCCGATCGCACGC15290.10037471476250122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA551.9603423E-413.817991
CGCATCG4300.013.69793413
CGCCAGT4250.013.63555218
ATCGCCA4400.013.602530516
TCGCCAG4350.013.54048717
CCGCATC4450.013.44969412
CGAGCCG3700.013.09484315
GCATCGC4700.012.53215314
CGACCAT1750.012.48554110
GTATCAA16800.012.3837571
GCCGCCT3950.012.26605518
ACTAAAC701.0910141E-412.2141173
CATCGCC5050.012.03983215
GGTATCA6050.011.933721
CCGCCTG4300.011.70952419
TTACCCT658.024192E-411.6921464
CCGACCA1800.011.610959
GATTTAC752.0740293E-411.3998431
GTATTAG3850.011.35049251
CATAGGA1104.968224E-711.2271172
Produced by FastQC (version 0.11.5)