FastQCFastQC Report
Mon 15 Aug 2016
SRR2920840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523292
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTGGAGCTGGAATTACCGCGGC25860.16976390606659786No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG24920.16359306029310205No Hit
CTTTAATATACGCTATTGGAGCTGG24480.1607045792927423No Hit
GTCCTATTCCATTATTCCTAGCTGC24060.157947392883308No Hit
GAATAGGACCGCGGTTCTATTTTGT23730.15578103213303818No Hit
GAACTACGACGGTATCTGATCGTCT23430.15381161326915654No Hit
GAATAACGCCGCCGCATCGCCAGTC23280.1528269038372157No Hit
GTATCTGATCGTCTTCGAACCTCCG22870.15013536472324412No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21890.14370192976789742No Hit
GATTAAGAGGGACGGCCGGGGGCAT21200.13917226638096963No Hit
CTCTTAATCATGGCCTCAGTTCCGA20800.13654637456246077No Hit
ATACAGGACTCTTTCGAGGCCCTGT20160.1323449476528466No Hit
CTCTAGATAACCTCGGGCCGATCGC19830.1301785869025768No Hit
GCTTTGAACACTCTAATTTTTTCAA19160.12578021810657444No Hit
ATATTAAAGTTGCTGCAGTTAAAAA19000.1247298613791709No Hit
GTCCTGTATTGTTATTTTTCGTCAC18750.12308867899260287No Hit
GTATCAACGCAGAGTACTTTTTTTT18740.12302303169714016No Hit
GTACATGGGTACCTGGTTGATCCTG18430.12098796553779577No Hit
CCATTATTCCTAGCTGCGGTATCCA17390.11416064680967272No Hit
GCTAAGAGCATCGAGGGGGCGCCGA16860.1106813401501485No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG16110.10575779299044438No Hit
CTTTAAATCCTTTAACGAGGATCCA16050.10536390921766804No Hit
GTACATGGGAATAACGCCGCCGCAT15790.10365707953563728No Hit
AGATAACCTCGGGCCGATCGCACGC15750.10339449035378641No Hit
GAATAATGGAATAGGACCGCGGTTC15730.10326319576286097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA602.5681951E-514.2499999
CGCATCG3700.014.12162113
ATCGCCA3850.013.57142716
GCATCGC3950.013.46835514
CGCCAGT3900.013.39743518
GGTATCA6350.013.31496051
CGTTATT1001.0069925E-813.2999992
TCGCCAG3900.013.15384617
CATCGCC4000.012.82515
GACCATA2700.012.66666611
GTATCAA18450.012.5636851
CCGCATC4350.012.22988412
CCGACCA2800.012.2142869
CGACCAT2600.012.057693510
GCTCGTA2150.011.9302319
CGAGCCG5450.011.50458715
ACGGTAT4100.011.353669
GCGTTAT1354.7257345E-911.2592591
CGTCGTA2450.011.24489810
CGGTTCT4600.011.15217512
Produced by FastQC (version 0.11.5)