##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920840_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1523292
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.360815917105846	33.0	32.0	34.0	32.0	34.0
2	32.55888431108415	33.0	32.0	34.0	32.0	34.0
3	32.75731245224159	33.0	32.0	34.0	32.0	34.0
4	32.92255982438036	33.0	32.0	34.0	32.0	34.0
5	32.64487570341077	33.0	32.0	34.0	32.0	34.0
6	35.56115242514239	37.0	35.0	37.0	33.0	37.0
7	35.50859323097607	37.0	35.0	37.0	33.0	37.0
8	35.35744098964611	37.0	34.0	37.0	33.0	37.0
9	35.3922130491068	37.0	34.0	37.0	33.0	37.0
10	35.313518353670865	37.0	34.0	37.0	33.0	37.0
11	35.29959981408686	37.0	34.0	37.0	33.0	37.0
12	35.212850195497644	37.0	34.0	37.0	33.0	37.0
13	37.098532651651816	38.0	38.0	38.0	36.0	38.0
14	37.06325116917833	38.0	38.0	38.0	36.0	38.0
15	37.00631330040465	38.0	38.0	38.0	36.0	38.0
16	37.085691384186354	38.0	38.0	38.0	36.0	38.0
17	37.046293159814404	38.0	38.0	38.0	36.0	38.0
18	37.0961109229222	38.0	38.0	38.0	36.0	38.0
19	37.00764068871891	38.0	38.0	38.0	36.0	38.0
20	37.064601534046	38.0	38.0	38.0	36.0	38.0
21	37.15445430029174	38.0	38.0	38.0	36.0	38.0
22	37.11280765605019	38.0	38.0	38.0	36.0	38.0
23	37.577443457984415	39.0	38.0	39.0	36.0	39.0
24	37.59052039924059	39.0	38.0	39.0	36.0	39.0
25	37.451585119596245	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	10.0
17	10.0
18	12.0
19	13.0
20	8.0
21	24.0
22	76.0
23	207.0
24	609.0
25	1281.0
26	2702.0
27	5144.0
28	8420.0
29	13120.0
30	19441.0
31	27309.0
32	38092.0
33	55470.0
34	94718.0
35	248703.0
36	865365.0
37	142555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33279502551054	18.345005422466606	11.974657518059571	26.347542033963283
2	16.681109071668466	20.35991786210392	37.067088910071085	25.891884156156536
3	18.608644961044895	24.363746412375303	29.00126830574834	28.026340320831462
4	13.299945118860993	15.891109518070074	36.42440188749104	34.38454347557789
5	15.092707110652457	35.76372750595421	32.93432907151091	16.20923631188242
6	33.05466056409408	35.88346817287821	17.1980815234374	13.863789739590308
7	29.391672771865146	30.740527751737684	21.314101301654574	18.553698174742596
8	27.499586422038586	32.78708218778802	20.062207377180474	19.651124012992913
9	27.22038847443563	15.16301536409303	19.403765003689376	38.21283115778196
10	16.297531924279784	26.686544667732782	30.591311449150915	26.42461195883652
11	36.51703022138894	21.70595000827156	22.325266593666875	19.45175317667263
12	25.011291334819585	23.62869364507921	28.419370678766775	22.940644341334426
13	28.96332416897089	19.452212707740866	25.50732229933591	26.077140823952334
14	23.691780696018885	20.48825832473354	25.14691864724557	30.673042332002005
15	24.848174117749007	26.955781922167205	22.37011838184562	25.825925578238166
16	25.739518725283727	26.071038437905376	23.417764729978835	24.771678106832063
17	23.891085885043708	26.114822371547934	25.292064817513648	24.702026925894707
18	24.775551896812956	24.927984916877392	26.319051107732466	23.97741207857719
19	26.259902894520554	24.679575550846454	25.239415686552547	23.821105868080448
20	25.647938806217063	24.673995530732125	24.736623050603562	24.94144261244725
21	27.031915089162155	24.49510665059621	24.216105644879644	24.256872615361992
22	25.33841180811033	24.377991875490714	24.986345362543755	25.297250953855205
23	23.901195568544967	23.90414969684079	25.61708457734958	26.577570157264663
24	24.76288196878865	25.25044443219028	25.133526598971173	24.85314700004989
25	24.914067690239296	24.540534579056413	25.245914768803356	25.29948296190094
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	78.0
1	78.0
2	163.0
3	248.0
4	248.0
5	248.0
6	728.5
7	1209.0
8	1209.0
9	1209.0
10	1697.0
11	2185.0
12	2185.0
13	2185.0
14	3264.0
15	4343.0
16	4343.0
17	4343.0
18	7608.0
19	10873.0
20	10873.0
21	10873.0
22	18021.0
23	25169.0
24	25169.0
25	25169.0
26	36952.0
27	48735.0
28	48735.0
29	48735.0
30	60111.0
31	71487.0
32	71487.0
33	71487.0
34	90080.0
35	108673.0
36	108673.0
37	108673.0
38	118467.0
39	128261.0
40	128261.0
41	128261.0
42	142066.5
43	155872.0
44	155872.0
45	155872.0
46	175216.5
47	194561.0
48	194561.0
49	194561.0
50	194848.0
51	195135.0
52	195135.0
53	195135.0
54	181611.5
55	168088.0
56	168088.0
57	168088.0
58	154094.5
59	140101.0
60	140101.0
61	140101.0
62	125530.0
63	110959.0
64	110959.0
65	110959.0
66	91006.0
67	71053.0
68	71053.0
69	71053.0
70	53070.5
71	35088.0
72	35088.0
73	35088.0
74	27973.0
75	20858.0
76	20858.0
77	20858.0
78	19219.0
79	17580.0
80	17580.0
81	17580.0
82	12472.0
83	7364.0
84	7364.0
85	7364.0
86	5495.5
87	3627.0
88	3627.0
89	3627.0
90	2652.0
91	1677.0
92	1677.0
93	1677.0
94	872.0
95	67.0
96	67.0
97	67.0
98	34.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	6.564729546272153E-5
16	2.625891818508861E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1523292.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.61051654334128
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.723663209312	52.587502468585626
2	6.113536826363076	7.166351025997583
3	1.6144679053416866	2.838743936241674
4	0.7463850591776723	1.749840554345429
5	0.4254243058143828	1.2467169156936682
6	0.2536784601380203	0.8920935350765267
7	0.1790741513803885	0.7346939958375542
8	0.1278914286748009	0.5996626156876776
9	0.10481495351502425	0.5528932710283688
>10	0.5377409841757037	5.985868038253247
>50	0.06543924360367302	2.737795573014139
>100	0.08615001903640324	10.690237566513542
>500	0.014227570053221957	5.558403169951251
>1k	0.007505883413904495	6.6591973337737835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTATTGGAGCTGGAATTACCGCGGC	2586	0.16976390606659786	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2492	0.16359306029310205	No Hit
CTTTAATATACGCTATTGGAGCTGG	2448	0.1607045792927423	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2406	0.157947392883308	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2373	0.15578103213303818	No Hit
GAACTACGACGGTATCTGATCGTCT	2343	0.15381161326915654	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2328	0.1528269038372157	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2287	0.15013536472324412	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2189	0.14370192976789742	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2120	0.13917226638096963	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	2080	0.13654637456246077	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	2016	0.1323449476528466	No Hit
CTCTAGATAACCTCGGGCCGATCGC	1983	0.1301785869025768	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1916	0.12578021810657444	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1900	0.1247298613791709	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1875	0.12308867899260287	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1874	0.12302303169714016	No Hit
GTACATGGGTACCTGGTTGATCCTG	1843	0.12098796553779577	No Hit
CCATTATTCCTAGCTGCGGTATCCA	1739	0.11416064680967272	No Hit
GCTAAGAGCATCGAGGGGGCGCCGA	1686	0.1106813401501485	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1611	0.10575779299044438	No Hit
CTTTAAATCCTTTAACGAGGATCCA	1605	0.10536390921766804	No Hit
GTACATGGGAATAACGCCGCCGCAT	1579	0.10365707953563728	No Hit
AGATAACCTCGGGCCGATCGCACGC	1575	0.10339449035378641	No Hit
GAATAATGGAATAGGACCGCGGTTC	1573	0.10326319576286097	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.564729546272153E-5	0.0
5	0.0	0.0	0.0	6.564729546272153E-5	0.0
6	0.0	0.0	0.0	6.564729546272153E-5	0.0
7	0.0	0.0	0.0	6.564729546272153E-5	0.0
8	0.0	0.0	0.0	6.564729546272153E-5	0.0
9	0.0	0.0	0.0	6.564729546272153E-5	0.0
10	0.0	0.0	0.0	6.564729546272153E-5	0.0
11	0.0	0.0	0.0	6.564729546272153E-5	0.0
12	0.0	0.0	0.0	6.564729546272153E-5	6.564729546272153E-5
13	0.0	0.0	0.0	6.564729546272153E-5	6.564729546272153E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCCA	60	2.5681951E-5	14.249999	9
CGCATCG	370	0.0	14.121621	13
ATCGCCA	385	0.0	13.571427	16
GCATCGC	395	0.0	13.468355	14
CGCCAGT	390	0.0	13.397435	18
GGTATCA	635	0.0	13.3149605	1
CGTTATT	100	1.0069925E-8	13.299999	2
TCGCCAG	390	0.0	13.153846	17
CATCGCC	400	0.0	12.825	15
GACCATA	270	0.0	12.666666	11
GTATCAA	1845	0.0	12.563685	1
CCGCATC	435	0.0	12.229884	12
CCGACCA	280	0.0	12.214286	9
CGACCAT	260	0.0	12.0576935	10
GCTCGTA	215	0.0	11.930231	9
CGAGCCG	545	0.0	11.504587	15
ACGGTAT	410	0.0	11.35366	9
GCGTTAT	135	4.7257345E-9	11.259259	1
CGTCGTA	245	0.0	11.244898	10
CGGTTCT	460	0.0	11.152175	12
>>END_MODULE