FastQCFastQC Report
Mon 15 Aug 2016
SRR2920839_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920839_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383672
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC250.00602398419.04
CCAAGAC456.7459245E-414.7777773
TGTTAAG400.005269519514.252
CTAGCAC400.005269519514.253
GTATTAA852.681154E-713.4117651
TGTAGCA500.001495960813.2999992
GGCGAGA655.4316413E-513.15384619
GTCCTAA802.8532004E-511.8751
CTAAATT657.9992827E-411.69230719
TTAAAGG600.00586200211.0833333
GGACTGT700.001486972210.8571436
GTCTACA700.001486972210.8571431
AAAGTGT700.001486972210.8571436
GTCCTAC1502.1045707E-910.7666661
TTAGGAC803.7567434E-410.68753
CTAAAGT803.7567434E-410.68754
ATATACT803.7567434E-410.68754
TAGGACG909.47138E-510.5555544
TCCTACA1553.614332E-910.4193552
TGGCGAG1106.0057755E-610.36363618
Produced by FastQC (version 0.11.5)