##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920839_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	383672
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79667789153235	33.0	32.0	34.0	32.0	34.0
2	32.90981619716842	33.0	32.0	34.0	32.0	34.0
3	33.17502189370087	33.0	33.0	34.0	32.0	34.0
4	33.28103953376843	34.0	33.0	34.0	32.0	34.0
5	33.26202850351342	34.0	33.0	34.0	32.0	34.0
6	36.127734106215726	37.0	37.0	37.0	33.0	37.0
7	36.09584749473509	37.0	37.0	37.0	33.0	37.0
8	36.08206228236619	37.0	37.0	37.0	33.0	37.0
9	36.05576377739319	37.0	37.0	37.0	33.0	37.0
10	36.054658666777875	37.0	37.0	37.0	33.0	37.0
11	35.973326174440665	37.0	35.0	37.0	33.0	37.0
12	35.955547968056045	37.0	35.0	37.0	33.0	37.0
13	37.69507026835422	38.0	38.0	38.0	38.0	38.0
14	37.69945943409996	38.0	38.0	38.0	38.0	38.0
15	37.702018911987324	38.0	38.0	38.0	38.0	38.0
16	37.68038063762798	38.0	38.0	38.0	38.0	38.0
17	37.67629902625159	38.0	38.0	38.0	38.0	38.0
18	37.67177693446486	38.0	38.0	38.0	38.0	38.0
19	37.683904480910776	38.0	38.0	38.0	38.0	38.0
20	37.69375925269501	38.0	38.0	38.0	38.0	38.0
21	37.70317093767593	38.0	38.0	38.0	38.0	38.0
22	37.68153005692362	38.0	38.0	38.0	38.0	38.0
23	38.37165339143852	39.0	38.0	39.0	38.0	39.0
24	38.383642799057526	39.0	38.0	39.0	38.0	39.0
25	38.28463635605413	39.0	38.0	39.0	38.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	4.0
19	21.0
20	37.0
21	77.0
22	103.0
23	188.0
24	263.0
25	279.0
26	364.0
27	481.0
28	607.0
29	740.0
30	957.0
31	1409.0
32	2111.0
33	3534.0
34	8253.0
35	35619.0
36	268284.0
37	60339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46309347567714	17.975510331741695	12.55812256302258	27.00327362955858
2	15.892481077587107	19.422058424904606	38.910840509601954	25.774619987906338
3	19.284962155174213	21.94973831814675	28.761806960111763	30.003492566567274
4	13.22301340728539	15.46320815696741	36.26926124397924	35.04451719176797
5	14.745407535603327	35.29994370191205	35.174576200504596	14.780072561980024
6	33.76868783752789	35.06093746742009	17.150065681102607	14.020309013949413
7	29.324527200316936	29.88229529389687	21.20639504576826	19.586782460017933
8	25.547863800329445	34.75677140891178	20.264965908380077	19.4303988823787
9	24.96168602347839	16.04808273733814	19.607372964407098	39.382858274776375
10	14.637242227736191	27.476594591213328	33.65765549740403	24.22850768364645
11	34.899601743155614	21.94061594278446	24.110438082528827	19.049344231531098
12	23.35797243478805	23.32278612981922	30.3248608186159	22.99438061677683
13	27.808388415104567	20.749754999061697	25.93725890865114	25.504597677182595
14	21.647136095414833	20.361402447924267	26.581819887820846	31.40964156884005
15	23.282908317521215	28.791519839863216	22.965189015617508	24.960382826998064
16	23.97177797702204	27.214130820075482	25.02893096186326	23.78516024103922
17	22.699597572926873	27.289194937342316	26.92091161200192	23.090295877728895
18	22.821055484893346	26.83281552993182	27.256354386038073	23.08977459913676
19	23.785420880335288	26.50154298463271	26.337861506703643	23.375174628328363
20	23.472914364352885	26.581819887820846	26.50258554181697	23.4426802060093
21	22.98134865197356	26.21796743051356	26.616745553493608	24.183938364019266
22	23.308190329239558	26.179132175399822	26.45619174711733	24.056485748243293
23	23.0345190683709	26.02926458016222	26.85262411643279	24.08359223503409
24	23.346764945057235	26.039168873412706	26.721522550511896	23.892543631018164
25	23.4087970975208	26.027440105089767	26.57374006964282	23.99002272774662
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	3.0
2	5.0
3	7.0
4	7.0
5	7.0
6	26.0
7	45.0
8	45.0
9	45.0
10	133.5
11	222.0
12	222.0
13	222.0
14	479.5
15	737.0
16	737.0
17	737.0
18	1506.0
19	2275.0
20	2275.0
21	2275.0
22	3939.0
23	5603.0
24	5603.0
25	5603.0
26	8853.0
27	12103.0
28	12103.0
29	12103.0
30	16526.5
31	20950.0
32	20950.0
33	20950.0
34	25830.0
35	30710.0
36	30710.0
37	30710.0
38	35232.0
39	39754.0
40	39754.0
41	39754.0
42	43412.5
43	47071.0
44	47071.0
45	47071.0
46	49475.5
47	51880.0
48	51880.0
49	51880.0
50	51040.5
51	50201.0
52	50201.0
53	50201.0
54	47218.5
55	44236.0
56	44236.0
57	44236.0
58	39049.5
59	33863.0
60	33863.0
61	33863.0
62	28168.0
63	22473.0
64	22473.0
65	22473.0
66	17539.5
67	12606.0
68	12606.0
69	12606.0
70	9239.0
71	5872.0
72	5872.0
73	5872.0
74	3995.0
75	2118.0
76	2118.0
77	2118.0
78	1404.5
79	691.0
80	691.0
81	691.0
82	447.5
83	204.0
84	204.0
85	204.0
86	121.0
87	38.0
88	38.0
89	38.0
90	23.5
91	9.0
92	9.0
93	9.0
94	5.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	383672.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.1838332411329
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.0699371725772	87.63911234561839
2	3.5609195792313737	6.565184333539002
3	0.7228854814167496	1.999150640140732
4	0.27302736242642	1.0067483539273383
5	0.1352118886361809	0.6232175097128166
6	0.06455812196436149	0.35707290897140515
7	0.04778746201256677	0.30836620004284004
8	0.02704394215587315	0.19944114031038837
9	0.02298135874606138	0.19066587680714162
>10	0.0753376036083214	1.0930356071919194
>50	3.1002722486216566E-4	0.01800508373805021
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.606392960653892E-4
13	0.0	0.0	0.0	0.0	2.606392960653892E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	25	0.006023984	19.0	4
CCAAGAC	45	6.7459245E-4	14.777777	3
TGTTAAG	40	0.0052695195	14.25	2
CTAGCAC	40	0.0052695195	14.25	3
GTATTAA	85	2.681154E-7	13.411765	1
TGTAGCA	50	0.0014959608	13.299999	2
GGCGAGA	65	5.4316413E-5	13.153846	19
GTCCTAA	80	2.8532004E-5	11.875	1
CTAAATT	65	7.9992827E-4	11.692307	19
TTAAAGG	60	0.005862002	11.083333	3
GGACTGT	70	0.0014869722	10.857143	6
GTCTACA	70	0.0014869722	10.857143	1
AAAGTGT	70	0.0014869722	10.857143	6
GTCCTAC	150	2.1045707E-9	10.766666	1
TTAGGAC	80	3.7567434E-4	10.6875	3
CTAAAGT	80	3.7567434E-4	10.6875	4
ATATACT	80	3.7567434E-4	10.6875	4
TAGGACG	90	9.47138E-5	10.555554	4
TCCTACA	155	3.614332E-9	10.419355	2
TGGCGAG	110	6.0057755E-6	10.363636	18
>>END_MODULE