FastQCFastQC Report
Mon 15 Aug 2016
SRR2920839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383672
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGA456.7459245E-414.7777771
GTATTAA500.001495960813.2999991
GTCCTAG500.001495960813.2999991
CCATATG500.001495960813.2999993
CGCAGAG500.001495960813.2999992
CTTACAC500.001495960813.2999993
AGAGTAC604.0795578E-412.6666665
TATACAG600.00586200211.0833335
CTGCTCA1053.4434652E-610.8571429
TACACTG803.7567434E-410.68755
ACTGGTG803.7567434E-410.68758
AGACTGT909.47138E-510.5555546
CCAGGAC1201.5120022E-610.2916663
TTCTACA750.002639807310.1333332
GGGCTGT856.5688655E-410.0588246
GTACAGA856.5688655E-410.0588241
ATACAGA1054.0823565E-59.952386
GTTTAAA800.00450237759.51
CAGAGTA1002.7427092E-49.54
ACACCAT900.00110949429.4999996
Produced by FastQC (version 0.11.5)