##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920839_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	383672
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.876243249442233	32.0	32.0	33.0	29.0	33.0
2	31.373543026334996	32.0	32.0	33.0	29.0	34.0
3	31.70859744782001	32.0	32.0	33.0	30.0	34.0
4	31.59521674763861	32.0	32.0	33.0	30.0	34.0
5	31.657738901978774	33.0	32.0	33.0	30.0	34.0
6	34.76463229008111	37.0	34.0	37.0	32.0	37.0
7	34.873980900352386	37.0	34.0	37.0	32.0	37.0
8	34.843006526407976	37.0	34.0	37.0	32.0	37.0
9	34.81750036489501	37.0	33.0	37.0	32.0	37.0
10	34.77461738151337	37.0	33.0	37.0	32.0	37.0
11	34.77630371775892	37.0	33.0	37.0	32.0	37.0
12	34.7342938760191	37.0	33.0	37.0	32.0	37.0
13	36.302834191705415	38.0	37.0	38.0	33.0	38.0
14	36.253362246919245	38.0	37.0	38.0	33.0	38.0
15	36.285890031068206	38.0	37.0	38.0	33.0	38.0
16	36.324952042369524	38.0	37.0	38.0	33.0	38.0
17	36.31781313205029	38.0	37.0	38.0	33.0	38.0
18	36.392859004566404	38.0	37.0	38.0	34.0	38.0
19	36.48216966575617	38.0	37.0	38.0	34.0	38.0
20	36.36868470985634	38.0	37.0	38.0	34.0	38.0
21	36.2646531412248	38.0	37.0	38.0	33.0	38.0
22	36.33743927104402	38.0	37.0	38.0	33.0	38.0
23	36.98505233637065	38.0	37.0	39.0	34.0	39.0
24	36.80834931921016	38.0	37.0	39.0	34.0	39.0
25	36.685134698388204	38.0	37.0	39.0	33.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	2.0
21	13.0
22	46.0
23	137.0
24	314.0
25	700.0
26	1328.0
27	2470.0
28	4170.0
29	6389.0
30	9546.0
31	13561.0
32	19036.0
33	28421.0
34	45221.0
35	86399.0
36	156037.0
37	9881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18681582184783	18.837444483829938	13.506328322108468	25.46941137221377
2	15.910725828311683	21.76155674638754	37.545351237515376	24.782366187785403
3	20.14481119289393	23.406711983152277	28.946339581726054	27.502137242227736
4	14.34506557684689	17.211837194270103	35.64085990116558	32.80223732771743
5	14.955743447528096	36.550230405137725	34.64938801893284	13.844638128401343
6	34.20942888717446	35.78707854625826	16.69733522383703	13.306157342730248
7	29.912008173648324	30.594361850747514	20.770866782042997	18.722763193561168
8	26.180696011176213	35.584822452511524	19.7293521549657	18.505129381346567
9	25.589826726995973	16.532871828019765	19.48565441314456	38.391647031839696
10	15.156696344794513	28.526449675764713	33.08190329239559	23.234950687045185
11	35.70940803603078	22.517671344273232	23.615484059300655	18.157436560395336
12	23.861266915490315	23.970996059133842	29.955013657499112	22.212723367876727
13	28.481359077545402	21.149575679226004	25.871577806042662	24.49748743718593
14	22.056339790237494	20.82872870576951	26.547415500740218	30.567516003252777
15	23.79949540232282	29.373006109385102	22.592735461540066	24.234763026752017
16	24.25613544902938	27.92828249129465	24.924414604141038	22.891167455534937
17	23.118966200296086	27.917856919452035	26.45097896119602	22.51219791905586
18	23.401759836527035	27.400748556058304	26.99076294334744	22.206728664067224
19	24.43858295627515	27.006922579703495	26.164015096228027	22.390479367793322
20	24.01817177172168	27.141933735065372	26.34098917825643	22.498905314956524
21	23.797410287954293	26.541160157634646	26.344638128401343	23.31679142600972
22	24.032767572301342	26.62117642152672	26.043599741445817	23.30245626472612
23	23.584728622364935	26.719176796847304	26.262536750140747	23.43355783064701
24	23.908181988782083	26.667830855522425	26.23412706686962	23.189860088825874
25	24.035373965261993	26.698065013866014	26.198158844012593	23.0684021768594
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	7.5
3	13.0
4	13.0
5	13.0
6	39.0
7	65.0
8	65.0
9	65.0
10	219.0
11	373.0
12	373.0
13	373.0
14	601.0
15	829.0
16	829.0
17	829.0
18	1702.0
19	2575.0
20	2575.0
21	2575.0
22	4639.5
23	6704.0
24	6704.0
25	6704.0
26	9867.0
27	13030.0
28	13030.0
29	13030.0
30	17461.0
31	21892.0
32	21892.0
33	21892.0
34	26648.0
35	31404.0
36	31404.0
37	31404.0
38	35926.5
39	40449.0
40	40449.0
41	40449.0
42	44087.5
43	47726.0
44	47726.0
45	47726.0
46	49796.5
47	51867.0
48	51867.0
49	51867.0
50	50987.5
51	50108.0
52	50108.0
53	50108.0
54	46492.0
55	42876.0
56	42876.0
57	42876.0
58	37792.0
59	32708.0
60	32708.0
61	32708.0
62	27033.0
63	21358.0
64	21358.0
65	21358.0
66	16503.5
67	11649.0
68	11649.0
69	11649.0
70	8491.5
71	5334.0
72	5334.0
73	5334.0
74	3594.5
75	1855.0
76	1855.0
77	1855.0
78	1244.0
79	633.0
80	633.0
81	633.0
82	400.0
83	167.0
84	167.0
85	167.0
86	107.5
87	48.0
88	48.0
89	48.0
90	27.0
91	6.0
92	6.0
93	6.0
94	3.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	383672.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.3510708558939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.22909613031229	87.945090042732
2	3.416345071641949	6.310062515587791
3	0.7198954147161221	1.9944933735984514
4	0.27765424354252544	1.0256666687534155
5	0.12324530372879955	0.5690917888657265
6	0.07384997658727156	0.4092074652310332
7	0.045262072754468696	0.2926000621622794
8	0.032206141138692	0.23794172978354117
9	0.01773673277895511	0.1474205639059205
>10	0.06347400620513688	0.914465111842307
>50	6.17453297090294E-4	0.03449859627979258
>100	6.174532967033465E-4	0.11946208125774738
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.606392960653892E-4	0.0	0.0	0.0	0.0
3	2.606392960653892E-4	0.0	0.0	0.0	0.0
4	2.606392960653892E-4	0.0	0.0	0.0	0.0
5	2.606392960653892E-4	0.0	0.0	0.0	0.0
6	2.606392960653892E-4	0.0	0.0	0.0	0.0
7	2.606392960653892E-4	0.0	0.0	0.0	0.0
8	2.606392960653892E-4	0.0	0.0	0.0	0.0
9	2.606392960653892E-4	0.0	0.0	0.0	0.0
10	2.606392960653892E-4	0.0	0.0	0.0	0.0
11	2.606392960653892E-4	0.0	0.0	0.0	0.0
12	2.606392960653892E-4	0.0	0.0	0.0	2.606392960653892E-4
13	2.606392960653892E-4	0.0	0.0	0.0	2.606392960653892E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCAGA	45	6.7459245E-4	14.777777	1
GTATTAA	50	0.0014959608	13.299999	1
GTCCTAG	50	0.0014959608	13.299999	1
CCATATG	50	0.0014959608	13.299999	3
CGCAGAG	50	0.0014959608	13.299999	2
CTTACAC	50	0.0014959608	13.299999	3
AGAGTAC	60	4.0795578E-4	12.666666	5
TATACAG	60	0.005862002	11.083333	5
CTGCTCA	105	3.4434652E-6	10.857142	9
TACACTG	80	3.7567434E-4	10.6875	5
ACTGGTG	80	3.7567434E-4	10.6875	8
AGACTGT	90	9.47138E-5	10.555554	6
CCAGGAC	120	1.5120022E-6	10.291666	3
TTCTACA	75	0.0026398073	10.133333	2
GGGCTGT	85	6.5688655E-4	10.058824	6
GTACAGA	85	6.5688655E-4	10.058824	1
ATACAGA	105	4.0823565E-5	9.95238	6
GTTTAAA	80	0.0045023775	9.5	1
CAGAGTA	100	2.7427092E-4	9.5	4
ACACCAT	90	0.0011094942	9.499999	6
>>END_MODULE