FastQCFastQC Report
Mon 15 Aug 2016
SRR2920838_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920838_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576952
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACAC250.0060321918.9974863
TATACTG851.7120328E-814.527495
GGCGAGG3550.013.37851119
CTAATTG500.001499048813.2982413
GAATAAT604.003733E-412.6969011
TAGAACC604.0896615E-412.6649914
AGGACGT7800.012.2996555
TAGGACC1700.012.2924924
ACAATAC550.003067463912.089313
GACGTGA4250.012.0689927
TGGCGAG6850.012.06409718
GGACGTG8150.011.8879996
TGTAGGA7050.011.8565862
TAGGACG8750.011.7241644
CCTACAC658.018906E-411.6907623
TTACATC658.018906E-411.6907624
TGTAGAA2200.011.6575492
GTAGGAC6950.011.6171673
TTAGGAC4350.011.5731813
TATGGCG7350.011.37264416
Produced by FastQC (version 0.11.5)