##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920838_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	576952
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.047052441104285	32.0	32.0	33.0	29.0	34.0
2	31.445503265436294	32.0	32.0	33.0	29.0	34.0
3	31.5326092985205	33.0	32.0	33.0	29.0	34.0
4	31.73190664041376	33.0	32.0	33.0	30.0	34.0
5	31.621505775177138	33.0	32.0	33.0	30.0	34.0
6	34.61182906030311	37.0	34.0	37.0	32.0	37.0
7	34.64308642660048	37.0	34.0	37.0	32.0	37.0
8	34.457655056226514	37.0	33.0	37.0	31.0	37.0
9	34.66933644393294	37.0	34.0	37.0	32.0	37.0
10	34.54564677824152	37.0	33.0	37.0	32.0	37.0
11	34.59221044384975	37.0	33.0	37.0	32.0	37.0
12	34.627202956225126	37.0	33.0	37.0	32.0	37.0
13	36.05017055144969	38.0	37.0	38.0	33.0	38.0
14	35.82057606178677	38.0	37.0	38.0	32.0	38.0
15	36.02424811769436	38.0	37.0	38.0	33.0	38.0
16	36.05563374422829	38.0	37.0	38.0	33.0	38.0
17	35.93868814043456	38.0	37.0	38.0	33.0	38.0
18	35.8786086190879	38.0	37.0	38.0	33.0	38.0
19	36.04421684992859	38.0	37.0	38.0	33.0	38.0
20	35.957202678905695	38.0	37.0	38.0	33.0	38.0
21	35.94499195773652	38.0	37.0	38.0	33.0	38.0
22	35.844019953133014	38.0	37.0	38.0	33.0	38.0
23	36.35478861326419	38.0	37.0	39.0	33.0	39.0
24	36.312058888781046	38.0	37.0	39.0	33.0	39.0
25	35.532278248450474	38.0	36.0	39.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	4.0
18	21.0
19	112.0
20	369.0
21	873.0
22	1705.0
23	2860.0
24	4143.0
25	5535.0
26	7180.0
27	8704.0
28	10646.0
29	12804.0
30	15123.0
31	17942.0
32	22486.0
33	30033.0
34	46879.0
35	101152.0
36	264931.0
37	23447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.96408566778808	18.845384961416148	13.100446541000663	26.0900828297951
2	14.8407493171009	19.34320359405982	41.39287150404193	24.42317558479735
3	18.755633050929713	23.024445707788516	29.625341449548664	28.59457979173311
4	12.875767828172883	16.200307824567727	37.35562057155535	33.568303775704045
5	14.025776841054368	36.69438705472899	35.692743937103955	13.587092167112688
6	33.25649274116391	36.25656900400727	17.324838114782512	13.162100140046313
7	28.60359267322065	30.68868814043456	22.08277291698443	18.62494626936036
8	24.491985468461845	37.87160803671709	20.29544918814737	17.340957306673694
9	25.37455455566494	15.871684299560448	20.332020688029505	38.4217404567451
10	15.14406744408547	28.351751965501464	34.19937880447594	22.30480178593713
11	34.63529028411376	21.5900109541175	25.230001802576297	18.544696959192446
12	23.643041362193042	23.27749968801564	31.594482729932473	21.484976219858844
13	28.734502583408293	21.388644789592185	25.88417387265123	23.99267875434829
14	20.91460106664171	21.61414054226442	26.617511712433117	30.853746678660755
15	22.723979547621113	31.420400381315538	22.396394834907703	23.459225236155646
16	22.79465673861385	27.09068881066156	27.600957446992897	22.513697003731686
17	22.201538778856435	28.254045837514795	27.795081384727645	21.74933399890112
18	22.369451878145842	26.882825607676203	29.441790651562	21.305931862615953
19	24.20651977980837	26.276709327639043	26.16855474978854	23.34821614276404
20	23.86559020507772	27.197756485808178	26.983700550479067	21.952952758635032
21	22.90034526269083	26.44830072519031	26.22367198657774	24.42768202554112
22	23.483582689721157	27.749102178344128	26.346039185235515	22.421275946699208
23	22.452301057973628	27.265526421608733	27.30365784328679	22.978514677130853
24	24.095938656942	26.23944452918094	27.36657468905559	22.298042124821478
25	23.38426766871421	26.870519557952825	27.235367933554265	22.5098448397787
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	10.0
3	18.0
4	18.0
5	18.0
6	58.0
7	98.0
8	98.0
9	98.0
10	340.5
11	583.0
12	583.0
13	583.0
14	1325.5
15	2068.0
16	2068.0
17	2068.0
18	3828.5
19	5589.0
20	5589.0
21	5589.0
22	9308.0
23	13027.0
24	13027.0
25	13027.0
26	19081.0
27	25135.0
28	25135.0
29	25135.0
30	32899.5
31	40664.0
32	40664.0
33	40664.0
34	46801.5
35	52939.0
36	52939.0
37	52939.0
38	57839.5
39	62740.0
40	62740.0
41	62740.0
42	66099.0
43	69458.0
44	69458.0
45	69458.0
46	71603.5
47	73749.0
48	73749.0
49	73749.0
50	70906.0
51	68063.0
52	68063.0
53	68063.0
54	62315.0
55	56567.0
56	56567.0
57	56567.0
58	50459.0
59	44351.0
60	44351.0
61	44351.0
62	37112.5
63	29874.0
64	29874.0
65	29874.0
66	23639.0
67	17404.0
68	17404.0
69	17404.0
70	12988.0
71	8572.0
72	8572.0
73	8572.0
74	6086.0
75	3600.0
76	3600.0
77	3600.0
78	2603.5
79	1607.0
80	1607.0
81	1607.0
82	1067.0
83	527.0
84	527.0
85	527.0
86	380.5
87	234.0
88	234.0
89	234.0
90	157.0
91	80.0
92	80.0
93	80.0
94	41.0
95	2.0
96	2.0
97	2.0
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2067763002814792
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.7332464399118122E-4
14	1.7332464399118122E-4
15	3.4664928798236244E-4
16	1.7332464399118122E-4
17	1.7332464399118122E-4
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	576952.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.00246018940798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.05323449052713	90.29240351259647
2	2.920420548505552	5.490534326944443
3	0.5561322826515659	1.5683340827999523
4	0.21102863901106436	0.7934884494985011
5	0.0960282221188404	0.45134445633929615
6	0.05279039144908928	0.2977456002345767
7	0.03285845551934409	0.21621429597898034
8	0.018628237686468846	0.14008801372168936
9	0.013871661052949699	0.11735732393017786
>10	0.04500707147798404	0.6324899379559362
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	6.93298575964725E-4
13	0.0	0.0	0.0	0.0	8.666232199559062E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACAC	25	0.00603219	18.997486	3
TATACTG	85	1.7120328E-8	14.52749	5
GGCGAGG	355	0.0	13.378511	19
CTAATTG	50	0.0014990488	13.298241	3
GAATAAT	60	4.003733E-4	12.696901	1
TAGAACC	60	4.0896615E-4	12.664991	4
AGGACGT	780	0.0	12.299655	5
TAGGACC	170	0.0	12.292492	4
ACAATAC	55	0.0030674639	12.08931	3
GACGTGA	425	0.0	12.068992	7
TGGCGAG	685	0.0	12.064097	18
GGACGTG	815	0.0	11.887999	6
TGTAGGA	705	0.0	11.856586	2
TAGGACG	875	0.0	11.724164	4
CCTACAC	65	8.018906E-4	11.690762	3
TTACATC	65	8.018906E-4	11.690762	4
TGTAGAA	220	0.0	11.657549	2
GTAGGAC	695	0.0	11.617167	3
TTAGGAC	435	0.0	11.573181	3
TATGGCG	735	0.0	11.372644	16
>>END_MODULE