FastQCFastQC Report
Mon 15 Aug 2016
SRR2920838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576952
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCT350.002167909416.2857888
GGATAGC400.0052463814.261191
GTGCTAT602.5438363E-514.261191
CCGTGCA400.00527361914.2500669
CGCAGAG759.641899E-713.9333972
GGTTGAG500.001497503613.3000597
AGTCGTC801.9930922E-613.062568
ACGCAGA751.4629119E-512.6766141
GTGTAGT604.058129E-412.6766141
CGAAATC751.4747013E-512.66672413
TAGAAGT604.0849412E-412.6667244
AAGTCGT905.3849544E-712.6667247
CAAGTCG853.9309834E-612.2941746
GTCATAA550.003046458812.1004031
GTATTAC550.003046458812.1004031
TTTAGAA3000.012.0333882
CGTCAAG802.8589122E-511.87505411
GTAGAAC2500.011.7800533
TGTAGAA2500.011.7800532
AGGACTT907.4373493E-611.6111635
Produced by FastQC (version 0.11.5)