##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920838_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	576952
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27947212246426	33.0	32.0	34.0	32.0	34.0
2	32.343255591453016	33.0	32.0	34.0	32.0	34.0
3	32.58131179023558	33.0	32.0	34.0	32.0	34.0
4	32.70186081337789	33.0	32.0	34.0	32.0	34.0
5	32.649345179495	33.0	32.0	34.0	32.0	34.0
6	35.717330037854104	37.0	37.0	37.0	33.0	37.0
7	35.741408297397356	37.0	37.0	37.0	33.0	37.0
8	35.61855405648997	37.0	35.0	37.0	33.0	37.0
9	35.66307942428486	37.0	35.0	37.0	33.0	37.0
10	35.54440230729766	37.0	35.0	37.0	33.0	37.0
11	35.49576394570086	37.0	34.0	37.0	33.0	37.0
12	35.558536932015144	37.0	34.0	37.0	33.0	37.0
13	37.07480518310015	38.0	38.0	38.0	36.0	38.0
14	37.06796232615538	38.0	38.0	38.0	36.0	38.0
15	37.02195503265436	38.0	38.0	38.0	36.0	38.0
16	37.086896310264976	38.0	38.0	38.0	36.0	38.0
17	37.064766219720184	38.0	38.0	38.0	36.0	38.0
18	37.064402237967805	38.0	38.0	38.0	36.0	38.0
19	37.10055602545793	38.0	38.0	38.0	36.0	38.0
20	37.096195177415105	38.0	38.0	38.0	36.0	38.0
21	37.04455136649149	38.0	38.0	38.0	36.0	38.0
22	37.10079348022019	38.0	38.0	38.0	36.0	38.0
23	37.66203081018872	39.0	38.0	39.0	36.0	39.0
24	37.567470084166445	39.0	38.0	39.0	36.0	39.0
25	37.24873992983819	39.0	38.0	39.0	35.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	3.0
22	18.0
23	64.0
24	180.0
25	458.0
26	976.0
27	1911.0
28	3358.0
29	5484.0
30	7852.0
31	10868.0
32	14933.0
33	21033.0
34	33602.0
35	81099.0
36	333041.0
37	62071.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.350779828685695	20.08303835754707	13.258041522647376	26.30814029111986
2	15.68544864595033	20.14400210768612	39.94290527892176	24.227643967441796
3	19.278206852563127	23.701105117930087	29.486335085067733	27.53435294443905
4	13.822987007584686	16.867261054645795	36.935654959164715	32.374096978604804
5	14.038602864709715	36.95194747569989	35.569856764514206	13.439592895076194
6	33.388915659309234	36.52746818460727	17.034191797158023	13.04942435892547
7	28.403122757511362	30.8608036385949	22.274087144625135	18.461986459268605
8	24.531503487291836	38.07214464981489	20.06995382631484	17.326398036578432
9	25.152699011356233	15.847245524757692	20.46288079424285	38.53717466964323
10	15.047352292738392	28.815048738889892	34.08533118872974	22.052267779641983
11	34.577746502308685	21.667487069981558	25.223242141460638	18.531524286249116
12	23.62969536460572	23.433665192251695	31.45270317114769	21.483936271994896
13	28.765824539996395	21.25670766372246	26.073052870949404	23.904414925331743
14	20.82783746916972	21.784247827797603	26.541167416877403	30.846747286155274
15	22.710680486705723	31.421638298609906	22.405449440149756	23.462231774534615
16	22.734993552323242	27.4783690844299	27.225315104202775	22.561322259044083
17	21.982937922045508	28.61693867080797	27.72449007889738	21.675633328249145
18	22.457674121937355	27.00709937741788	29.22530817121702	21.309918329427752
19	24.294568698955892	26.345865860591523	26.23095162162537	23.128613818827215
20	23.869923321177495	27.306951011522624	26.86306659826121	21.960059069038675
21	22.846961272341545	26.58574716787532	26.30773443891346	24.259557120869673
22	23.635935051789403	27.654640247368935	26.325933526532534	22.38349117430913
23	22.406543351959954	27.524300115087563	27.145239118678848	22.92391741427363
24	24.08553917830253	26.470659604265173	27.207982639803657	22.235818577628642
25	23.387907486238024	27.025125140392962	27.150785507286567	22.436181866082446
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	13.5
3	25.0
4	25.0
5	25.0
6	63.5
7	102.0
8	102.0
9	102.0
10	409.0
11	716.0
12	716.0
13	716.0
14	1379.5
15	2043.0
16	2043.0
17	2043.0
18	3873.5
19	5704.0
20	5704.0
21	5704.0
22	9629.5
23	13555.0
24	13555.0
25	13555.0
26	19475.0
27	25395.0
28	25395.0
29	25395.0
30	33408.0
31	41421.0
32	41421.0
33	41421.0
34	47207.5
35	52994.0
36	52994.0
37	52994.0
38	57764.0
39	62534.0
40	62534.0
41	62534.0
42	66247.0
43	69960.0
44	69960.0
45	69960.0
46	71784.5
47	73609.0
48	73609.0
49	73609.0
50	70796.5
51	67984.0
52	67984.0
53	67984.0
54	62289.0
55	56594.0
56	56594.0
57	56594.0
58	50054.5
59	43515.0
60	43515.0
61	43515.0
62	36635.5
63	29756.0
64	29756.0
65	29756.0
66	23371.5
67	16987.0
68	16987.0
69	16987.0
70	12685.0
71	8383.0
72	8383.0
73	8383.0
74	5924.5
75	3466.0
76	3466.0
77	3466.0
78	2457.0
79	1448.0
80	1448.0
81	1448.0
82	957.5
83	467.0
84	467.0
85	467.0
86	338.5
87	210.0
88	210.0
89	210.0
90	142.0
91	74.0
92	74.0
93	74.0
94	41.0
95	8.0
96	8.0
97	8.0
98	4.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060836950040904614
2	0.0027731943038588995
3	0.0
4	0.0
5	0.0
6	0.005199739319735437
7	0.004506440743770712
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	5.199739319735438E-4
15	0.002079895727894175
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	576952.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.58953749765165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.8163146098563	89.67404569065933
2	3.0725363189404766	5.751145060687525
3	0.6113842209467529	1.7165749941530601
4	0.22769277597553286	0.8523864638042611
5	0.09800692661671474	0.45862114668123083
6	0.0600364661488938	0.3371271059921034
7	0.03641732379199216	0.23857963434163404
8	0.018981489162804746	0.14211750334108667
9	0.015137957544428784	0.12750790007179585
>10	0.04308718262826276	0.6024638508555129
>50	0.0	0.0
>100	4.047283878751291E-4	0.09943064941251585
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	1.7332464399118125E-4	0.0	0.0	0.0	0.0
13	1.7332464399118125E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCT	35	0.0021679094	16.285788	8
GGATAGC	40	0.00524638	14.26119	1
GTGCTAT	60	2.5438363E-5	14.26119	1
CCGTGCA	40	0.005273619	14.250066	9
CGCAGAG	75	9.641899E-7	13.933397	2
GGTTGAG	50	0.0014975036	13.300059	7
AGTCGTC	80	1.9930922E-6	13.06256	8
ACGCAGA	75	1.4629119E-5	12.676614	1
GTGTAGT	60	4.058129E-4	12.676614	1
CGAAATC	75	1.4747013E-5	12.666724	13
TAGAAGT	60	4.0849412E-4	12.666724	4
AAGTCGT	90	5.3849544E-7	12.666724	7
CAAGTCG	85	3.9309834E-6	12.294174	6
GTCATAA	55	0.0030464588	12.100403	1
GTATTAC	55	0.0030464588	12.100403	1
TTTAGAA	300	0.0	12.033388	2
CGTCAAG	80	2.8589122E-5	11.875054	11
GTAGAAC	250	0.0	11.780053	3
TGTAGAA	250	0.0	11.780053	2
AGGACTT	90	7.4373493E-6	11.611163	5
>>END_MODULE