##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920837_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	87955
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21561025524416	33.0	32.0	33.0	32.0	34.0
2	32.556000227389006	33.0	32.0	34.0	32.0	34.0
3	32.790063100449096	33.0	32.0	34.0	32.0	34.0
4	32.89054630208629	33.0	33.0	34.0	32.0	34.0
5	32.75164572792905	33.0	32.0	34.0	32.0	34.0
6	35.81077823887215	37.0	37.0	37.0	33.0	37.0
7	35.75966119038144	37.0	37.0	37.0	33.0	37.0
8	35.7375248706725	37.0	37.0	37.0	33.0	37.0
9	35.80507077482804	37.0	35.0	37.0	33.0	37.0
10	35.80613950315502	37.0	35.0	37.0	33.0	37.0
11	35.73747939287136	37.0	34.0	37.0	33.0	37.0
12	35.74391450173384	37.0	34.0	37.0	33.0	37.0
13	37.446250923767835	38.0	38.0	38.0	37.0	38.0
14	37.426399863566594	38.0	38.0	38.0	37.0	38.0
15	37.406560172815645	38.0	38.0	38.0	37.0	38.0
16	37.43689386618157	38.0	38.0	38.0	37.0	38.0
17	37.43549542379626	38.0	38.0	38.0	37.0	38.0
18	37.438599283724635	38.0	38.0	38.0	37.0	38.0
19	37.41144903643909	38.0	38.0	38.0	37.0	38.0
20	37.445159456540274	38.0	38.0	38.0	37.0	38.0
21	37.456824512534816	38.0	38.0	38.0	37.0	38.0
22	37.444784264680806	38.0	38.0	38.0	37.0	38.0
23	38.06689784548917	39.0	38.0	39.0	37.0	39.0
24	38.01121027798306	39.0	38.0	39.0	37.0	39.0
25	37.935762605878004	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	7.0
20	4.0
21	16.0
22	49.0
23	53.0
24	97.0
25	112.0
26	192.0
27	243.0
28	330.0
29	437.0
30	603.0
31	746.0
32	1119.0
33	1691.0
34	3182.0
35	11290.0
36	59150.0
37	8631.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.838326416917745	17.302029446876244	11.47063839463362	27.389005741572397
2	17.59081348416804	19.870388266727304	37.06668182593372	25.472116423170938
3	19.790802114717753	23.04246489682224	28.926155420385424	28.24057756807458
4	13.680859530441703	16.481155136149166	35.82513785458473	34.0128474788244
5	14.253879824910465	34.68932977090557	33.76271957250867	17.29407083167529
6	32.530271161389344	34.13450059689614	18.595872889545788	14.739355352168722
7	27.751691205730207	30.073332954351656	22.835540901597405	19.339434938320732
8	25.868910238189986	33.98328690807799	21.380251264851342	18.767551588880675
9	25.75748962537661	16.36859757830709	20.034108350861235	37.83980444545506
10	16.09118299130237	27.01267693707009	33.18287760786766	23.713262463759875
11	35.02359160934569	21.347279859018816	24.645557387300325	18.98357114433517
12	24.803592746290718	22.985617645386846	30.096071854925814	22.114717753396622
13	27.84946847819908	21.008470240463872	26.126996759706667	25.015064521630382
14	23.335796714228866	20.047751691205733	25.743846285032117	30.872605309533284
15	24.03615485191291	27.814223182309135	23.093627423114093	25.05599454266386
16	24.76493661531465	25.95077028025695	24.893411403558638	24.390881700869762
17	23.715536353817292	25.72224432948667	26.194076516400433	24.368142800295605
18	23.641634926951284	26.336194644988915	26.764822920811778	23.257347507248024
19	25.247001307486784	25.005968961400715	25.930305269740206	23.816724461372292
20	24.830879426979706	25.87686885339094	25.576715365812063	23.715536353817292
21	24.170314365300438	25.449377522596784	25.598317321357513	24.78199079074527
22	23.977033710420102	25.692683758740266	26.074697288386105	24.255585242453527
23	24.086180433176054	25.299300778807343	25.915524984367007	24.698993803649593
24	24.13052128929566	25.8996077539651	26.265704053209028	23.704166903530215
25	24.5409584446592	25.08555511341027	26.196350406457846	24.177136035472685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	3.0
3	6.0
4	6.0
5	6.0
6	8.0
7	10.0
8	10.0
9	10.0
10	19.5
11	29.0
12	29.0
13	29.0
14	94.0
15	159.0
16	159.0
17	159.0
18	297.0
19	435.0
20	435.0
21	435.0
22	823.5
23	1212.0
24	1212.0
25	1212.0
26	1901.5
27	2591.0
28	2591.0
29	2591.0
30	3493.5
31	4396.0
32	4396.0
33	4396.0
34	5260.0
35	6124.0
36	6124.0
37	6124.0
38	7335.0
39	8546.0
40	8546.0
41	8546.0
42	9499.5
43	10453.0
44	10453.0
45	10453.0
46	11083.0
47	11713.0
48	11713.0
49	11713.0
50	11946.5
51	12180.0
52	12180.0
53	12180.0
54	11476.5
55	10773.0
56	10773.0
57	10773.0
58	9558.0
59	8343.0
60	8343.0
61	8343.0
62	7069.0
63	5795.0
64	5795.0
65	5795.0
66	4502.5
67	3210.0
68	3210.0
69	3210.0
70	2266.5
71	1323.0
72	1323.0
73	1323.0
74	904.0
75	485.0
76	485.0
77	485.0
78	308.0
79	131.0
80	131.0
81	131.0
82	81.5
83	32.0
84	32.0
85	32.0
86	19.5
87	7.0
88	7.0
89	7.0
90	4.5
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	87955.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.10431470638395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.07284507553968	64.64442044227161
2	7.755305943261982	11.649138764140755
3	2.7112537467074387	6.108805639247342
4	1.2700959762632837	3.8155875163435846
5	0.6797057131611615	2.55244158944915
6	0.4284113960458991	1.9305326587459495
7	0.26340488661479305	1.3847990449661758
8	0.17106179418087133	1.0277983059519074
9	0.13321626449483787	0.9004604627366267
>10	0.50864391898029	5.71655960434313
>50	0.006055284749765358	0.2694559718037633
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE