##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920837_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	87955
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.242828719231426	33.0	32.0	34.0	32.0	34.0
2	32.42044227161617	33.0	32.0	34.0	32.0	34.0
3	32.668091637769315	33.0	32.0	34.0	32.0	34.0
4	32.57709055767154	33.0	32.0	34.0	32.0	34.0
5	32.477903473367064	33.0	32.0	34.0	32.0	34.0
6	35.501085782502415	37.0	35.0	37.0	33.0	37.0
7	35.354681371155706	37.0	35.0	37.0	33.0	37.0
8	35.36765391393326	37.0	34.0	37.0	33.0	37.0
9	35.44206696606219	37.0	34.0	37.0	33.0	37.0
10	35.46818259337161	37.0	34.0	37.0	33.0	37.0
11	35.37041669035302	37.0	34.0	37.0	33.0	37.0
12	35.34921266556762	37.0	34.0	37.0	33.0	37.0
13	37.055903587061565	38.0	38.0	38.0	36.0	38.0
14	37.097583991814	38.0	38.0	38.0	36.0	38.0
15	37.10755499971577	38.0	38.0	38.0	36.0	38.0
16	37.073821840714004	38.0	38.0	38.0	36.0	38.0
17	36.94150417827298	38.0	38.0	38.0	36.0	38.0
18	37.01254050366665	38.0	38.0	38.0	36.0	38.0
19	37.003285771132965	38.0	38.0	38.0	36.0	38.0
20	37.02919674833722	38.0	38.0	38.0	36.0	38.0
21	37.03136831334205	38.0	38.0	38.0	36.0	38.0
22	37.05530100619635	38.0	38.0	38.0	36.0	38.0
23	37.60947075208914	39.0	38.0	39.0	36.0	39.0
24	37.58062645671082	39.0	38.0	39.0	36.0	39.0
25	37.40057984196464	39.0	38.0	39.0	35.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	1.0
21	1.0
22	3.0
23	6.0
24	28.0
25	83.0
26	176.0
27	279.0
28	495.0
29	756.0
30	1220.0
31	1671.0
32	2333.0
33	3570.0
34	5982.0
35	14538.0
36	48095.0
37	8716.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.79347393553522	17.70678187709624	11.579785117389573	27.919959069978965
2	17.259962480814053	20.241032346086065	37.01893013472798	25.480075038371897
3	19.40992553010062	23.513160138707292	29.490080154624525	27.586834176567564
4	13.87982491046558	17.297481666761414	35.16229890284805	33.660394519924964
5	14.142459212097094	35.17707918822125	33.6080950486044	17.072366551077256
6	32.606446478312776	34.65067363992951	18.19794212949804	14.54493775225968
7	28.233755897902334	30.15291910636121	22.362571769654938	19.250753226081518
8	25.932579159797626	34.46876243533625	20.84588710135865	18.752771303507476
9	25.790461031209137	16.40043203911091	20.218293445511907	37.59081348416804
10	15.967255983173215	27.416292422261385	33.10897618100165	23.507475413563757
11	34.746177022340966	21.43596157125803	24.90478085384572	18.913080552555282
12	24.298789153544426	22.948098459439485	30.290489454834855	22.46262293218123
13	27.93019157523734	21.3882098800523	25.809789096697173	24.871809448013188
14	23.26075834233415	20.358137684042976	25.741572394974703	30.63953157864817
15	24.05093513728611	28.14734807572054	22.713887783525667	25.087829003467682
16	24.54209538968791	26.474901938491275	24.729691319424706	24.25331135239611
17	23.77010971519527	26.03490421238133	25.943948610084703	24.251037462338694
18	23.571144335171397	26.32255130464442	26.81257461201751	23.293729748166676
19	24.97299755556819	25.290205218577682	26.10880563924734	23.627991586606786
20	24.634187937013248	25.939400829969873	25.646069012563245	23.78034222045364
21	24.087317378204766	25.389119436075262	25.843897447558412	24.67966573816156
22	24.37610141549656	25.299300778807343	26.0451367176397	24.279461088056394
23	24.08959126826218	25.691546813711557	25.827980217156497	24.390881700869762
24	23.886078108123474	26.059917003012906	26.06219089307032	23.991813995793304
25	24.504576203740548	25.485759763515436	26.05650616792678	23.953157864817236
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	2.0
3	4.0
4	4.0
5	4.0
6	7.5
7	11.0
8	11.0
9	11.0
10	54.5
11	98.0
12	98.0
13	98.0
14	133.0
15	168.0
16	168.0
17	168.0
18	340.0
19	512.0
20	512.0
21	512.0
22	898.0
23	1284.0
24	1284.0
25	1284.0
26	1941.0
27	2598.0
28	2598.0
29	2598.0
30	3479.5
31	4361.0
32	4361.0
33	4361.0
34	5312.0
35	6263.0
36	6263.0
37	6263.0
38	7363.5
39	8464.0
40	8464.0
41	8464.0
42	9526.5
43	10589.0
44	10589.0
45	10589.0
46	11227.5
47	11866.0
48	11866.0
49	11866.0
50	11974.5
51	12083.0
52	12083.0
53	12083.0
54	11436.0
55	10789.0
56	10789.0
57	10789.0
58	9524.5
59	8260.0
60	8260.0
61	8260.0
62	6955.0
63	5650.0
64	5650.0
65	5650.0
66	4361.0
67	3072.0
68	3072.0
69	3072.0
70	2170.5
71	1269.0
72	1269.0
73	1269.0
74	858.0
75	447.0
76	447.0
77	447.0
78	295.0
79	143.0
80	143.0
81	143.0
82	81.5
83	20.0
84	20.0
85	20.0
86	11.5
87	3.0
88	3.0
89	3.0
90	2.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	87955.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.93945767722131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.92538649431827	64.39201864589847
2	7.855809931273042	11.774202717298618
3	2.773352752871209	6.235006537433915
4	1.2334440853852806	3.6973452333579675
5	0.7145783077693323	2.677505542607015
6	0.43238814801329023	1.9441759990904441
7	0.2639843429975877	1.3847990449661758
8	0.18509247037761897	1.1096583480188733
9	0.1350264742918696	0.9106929679949974
>10	0.4763855385128882	5.681314308453186
>50	0.004551454189613582	0.19328065488033655
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGGATG	30	7.643856E-4	19.0	8
TTTAGAA	45	6.6730514E-4	14.777778	2
GAATATG	70	1.0685205E-4	12.214286	13
AATATGG	80	2.7950571E-5	11.875001	14
ATATGGC	75	2.0317493E-4	11.400001	15
GGAATAT	75	2.0317493E-4	11.400001	12
GTAGGAC	60	0.005801318	11.083334	3
TGGAATA	85	6.462229E-4	10.058824	11
>>END_MODULE