FastQCFastQC Report
Mon 15 Aug 2016
SRR2920836_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920836_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246715
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC2570.104168777739497No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA250.006018020719.01
GTGTCAG400.005262462414.2500011
TGTGTAA400.005262462414.25000110
CTAGAGG500.001493320813.2999993
CTCAATA500.001493320813.2999992
TGGAGAG604.070384E-412.6666675
GGGTGAT701.0837446E-412.2142867
TAGAAGA701.0837446E-412.2142864
TAGAGGT550.00305588512.0909094
ATGGTCT951.0280401E-612.012
AGGGTGA802.8435308E-511.8750016
CCTAGAG657.9815224E-411.6923082
TTATCCT657.9815224E-411.6923084
TCTAGAA855.280672E-511.1764712
GTCTTGC951.3472816E-511.015
CCCCCCT700.001483712710.85714316
TCCCCCC700.001483712710.85714315
AGGATAA700.001483712710.8571435
CCAGTGA1053.4272198E-610.85714216
GTGATGG1158.701154E-710.739139
Produced by FastQC (version 0.11.5)