##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920836_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	246715
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60171858216971	33.0	32.0	33.0	29.0	34.0
2	32.007125630788565	33.0	32.0	34.0	30.0	34.0
3	32.358482459518065	33.0	32.0	34.0	32.0	34.0
4	32.45438258719575	33.0	32.0	34.0	32.0	34.0
5	32.64997264049612	33.0	32.0	34.0	32.0	34.0
6	35.432713860122	37.0	34.0	37.0	33.0	37.0
7	35.34674016577833	37.0	34.0	37.0	33.0	37.0
8	35.39801390268123	37.0	34.0	37.0	33.0	37.0
9	35.45452445128995	37.0	34.0	37.0	33.0	37.0
10	35.4352957866364	37.0	34.0	37.0	33.0	37.0
11	35.360387491640154	37.0	34.0	37.0	33.0	37.0
12	35.378371805524594	37.0	34.0	37.0	33.0	37.0
13	37.096119003708736	38.0	38.0	38.0	37.0	38.0
14	37.09836450965689	38.0	38.0	38.0	37.0	38.0
15	37.049563261252864	38.0	38.0	38.0	37.0	38.0
16	37.06477919867053	38.0	38.0	38.0	37.0	38.0
17	37.06498186166224	38.0	38.0	38.0	37.0	38.0
18	37.08770443629289	38.0	38.0	38.0	37.0	38.0
19	37.05477170013984	38.0	38.0	38.0	37.0	38.0
20	37.08108140972377	38.0	38.0	38.0	37.0	38.0
21	37.107557302960906	38.0	38.0	38.0	37.0	38.0
22	37.13488843402306	38.0	38.0	38.0	37.0	38.0
23	37.7221125590256	39.0	38.0	39.0	37.0	39.0
24	37.65169527592566	39.0	38.0	39.0	37.0	39.0
25	37.528763958413556	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	2.0
18	5.0
19	36.0
20	96.0
21	174.0
22	339.0
23	554.0
24	740.0
25	1059.0
26	1305.0
27	1555.0
28	1875.0
29	2342.0
30	2894.0
31	3468.0
32	4434.0
33	6189.0
34	11340.0
35	33754.0
36	153994.0
37	20558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.487363962466816	16.922765133858906	11.612994751028515	26.97687615264577
2	18.14806558174412	18.860223334616865	36.11332914496484	26.878381938674178
3	19.326348215552358	22.206189327766857	28.714508643576597	29.752953813104188
4	14.127231826196219	15.482641914759945	34.721845043876534	35.6682812151673
5	15.143384066635592	34.61970289605415	33.313337251484505	16.92357578582575
6	34.92937194738869	33.54802099588594	16.815759074235455	14.706847982489919
7	29.79024380357903	29.465577690857874	21.705611738240478	19.03856676732262
8	27.398009849421396	33.32630768295402	19.93271588675192	19.342966580872666
9	26.74624566807855	15.519931905234785	18.886974849522726	38.846847577163935
10	16.067121982854708	26.479946496970186	32.8634254098859	24.5895061102892
11	36.15264576535679	21.08870559147194	23.279492531868755	19.479156111302515
12	24.631660012565103	22.811746346999577	29.514622134851955	23.041971505583366
13	29.13523701436881	20.022698255071642	24.92268406866222	25.91938066189733
14	24.14405285450824	19.282573009342762	24.870802342784184	31.702571793364815
15	25.29598929939404	26.853251727702006	22.107289787811848	25.74346918509211
16	26.001256510548608	25.368137324443186	23.549034310844497	25.08157185416371
17	24.846888109762276	24.800680947652147	25.258699308919198	25.093731633666376
18	24.9295746103804	25.452445128994995	25.011045133048253	24.606935127576353
19	25.465820886447926	24.99158948584399	25.08481446203109	24.457775165676995
20	25.80710536448939	24.802302251585836	24.575725026852847	24.814867357071925
21	25.000911983462696	24.625580122813773	24.912956245060087	25.460551648663436
22	25.056846969174956	24.63287599051537	24.797438339784772	25.5128387005249
23	25.205196279107472	24.354822365887767	24.865127779016273	25.574853575988488
24	24.875666254585248	25.233974423930444	24.88661005613765	25.003749265346652
25	25.32517277020043	24.450073971991976	24.9936161157611	25.23113714204649
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	2.0
3	4.0
4	4.0
5	4.0
6	14.0
7	24.0
8	24.0
9	24.0
10	77.0
11	130.0
12	130.0
13	130.0
14	275.0
15	420.0
16	420.0
17	420.0
18	783.0
19	1146.0
20	1146.0
21	1146.0
22	1768.5
23	2391.0
24	2391.0
25	2391.0
26	3499.5
27	4608.0
28	4608.0
29	4608.0
30	6168.0
31	7728.0
32	7728.0
33	7728.0
34	10388.5
35	13049.0
36	13049.0
37	13049.0
38	16796.5
39	20544.0
40	20544.0
41	20544.0
42	24320.0
43	28096.0
44	28096.0
45	28096.0
46	31105.5
47	34115.0
48	34115.0
49	34115.0
50	35605.5
51	37096.0
52	37096.0
53	37096.0
54	35870.0
55	34644.0
56	34644.0
57	34644.0
58	30993.0
59	27342.0
60	27342.0
61	27342.0
62	22947.0
63	18552.0
64	18552.0
65	18552.0
66	14451.5
67	10351.0
68	10351.0
69	10351.0
70	7381.0
71	4411.0
72	4411.0
73	4411.0
74	2952.5
75	1494.0
76	1494.0
77	1494.0
78	984.0
79	474.0
80	474.0
81	474.0
82	279.0
83	84.0
84	84.0
85	84.0
86	48.0
87	12.0
88	12.0
89	12.0
90	6.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	246715.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.39110163509288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.62029133242369	55.33414070909687
2	7.634513914755933	9.984585506686722
3	2.7303441415741916	5.356206337813751
4	1.4275440749930342	3.733947187857766
5	0.8490833557759968	2.7761248007106967
6	0.5730183409770101	2.248218034415998
7	0.4369879297538786	2.0002585489491294
8	0.28360503415287475	1.4836196490011697
9	0.23043591483683035	1.3561612494723703
>10	1.1672649036512748	13.26636983716167
>50	0.040012372236884454	1.7751280942998116
>100	0.006898684868428354	0.6852400445340314
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCTAGAGGTGGAGCCCAGTGACAC	257	0.104168777739497	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAA	25	0.0060180207	19.0	1
GTGTCAG	40	0.0052624624	14.250001	1
TGTGTAA	40	0.0052624624	14.250001	10
CTAGAGG	50	0.0014933208	13.299999	3
CTCAATA	50	0.0014933208	13.299999	2
TGGAGAG	60	4.070384E-4	12.666667	5
GGGTGAT	70	1.0837446E-4	12.214286	7
TAGAAGA	70	1.0837446E-4	12.214286	4
TAGAGGT	55	0.003055885	12.090909	4
ATGGTCT	95	1.0280401E-6	12.0	12
AGGGTGA	80	2.8435308E-5	11.875001	6
CCTAGAG	65	7.9815224E-4	11.692308	2
TTATCCT	65	7.9815224E-4	11.692308	4
TCTAGAA	85	5.280672E-5	11.176471	2
GTCTTGC	95	1.3472816E-5	11.0	15
CCCCCCT	70	0.0014837127	10.857143	16
TCCCCCC	70	0.0014837127	10.857143	15
AGGATAA	70	0.0014837127	10.857143	5
CCAGTGA	105	3.4272198E-6	10.857142	16
GTGATGG	115	8.701154E-7	10.73913	9
>>END_MODULE