FastQCFastQC Report
Mon 15 Aug 2016
SRR2920836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246715
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC2620.10619540765660783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAGA250.006018020719.02
GCCAATT250.006018020719.02
CTAGAGG456.733849E-414.7777783
CCCTAGA456.733849E-414.7777781
AGGAGAG500.001493320813.2999995
CCTAGAG500.001493320813.2999992
CTGAACC500.001493320813.2999994
TTACACA500.001493320813.2999994
TCTATGG500.001493320813.2999993
CTTACAC550.00305588512.0909093
CCAAGAC600.00585194711.0833343
TGAGCCT600.00585194711.08333410
AGAAGAG909.439947E-510.5555555
ACCTGCT750.002634094810.1333348
GCCCAGT1151.0149715E-59.91304314
GTGCTCA800.00449275979.5000019
GTACAAG1002.7337964E-49.51
GAGCCCA1201.6815007E-59.512
CCAGTGA1002.7337964E-49.516
GTGACAC1002.7337964E-49.519
Produced by FastQC (version 0.11.5)