##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920835_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	150103
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83176218996289	33.0	32.0	33.0	30.0	34.0
2	31.8402963298535	33.0	32.0	34.0	29.0	34.0
3	32.160123381944395	33.0	32.0	34.0	32.0	34.0
4	32.252300087273404	33.0	32.0	34.0	32.0	34.0
5	32.423995523074154	33.0	32.0	34.0	32.0	34.0
6	35.10535432336462	37.0	34.0	37.0	32.0	37.0
7	35.182268175852585	37.0	34.0	37.0	32.0	37.0
8	35.27814234225831	37.0	34.0	37.0	33.0	37.0
9	35.35446326855559	37.0	34.0	37.0	33.0	37.0
10	35.33395734928682	37.0	34.0	37.0	33.0	37.0
11	35.25442529463102	37.0	34.0	37.0	33.0	37.0
12	35.268342404881984	37.0	34.0	37.0	33.0	37.0
13	36.9804600840756	38.0	38.0	38.0	36.0	38.0
14	36.99172568169857	38.0	38.0	38.0	36.0	38.0
15	36.93152701811423	38.0	38.0	38.0	36.0	38.0
16	36.97312512075042	38.0	38.0	38.0	36.0	38.0
17	36.97518370718773	38.0	38.0	38.0	36.0	38.0
18	36.99750837758073	38.0	38.0	38.0	36.0	38.0
19	36.920900981326156	38.0	38.0	38.0	36.0	38.0
20	36.960187338027886	38.0	38.0	38.0	36.0	38.0
21	36.974137758739	38.0	38.0	38.0	36.0	38.0
22	37.024183394069404	38.0	38.0	38.0	36.0	38.0
23	37.60157358613752	39.0	38.0	39.0	36.0	39.0
24	37.50641892567104	39.0	38.0	39.0	36.0	39.0
25	37.39364303178484	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	2.0
17	2.0
18	7.0
19	20.0
20	56.0
21	132.0
22	258.0
23	323.0
24	549.0
25	762.0
26	852.0
27	1080.0
28	1348.0
29	1637.0
30	1925.0
31	2460.0
32	3072.0
33	4352.0
34	7329.0
35	22408.0
36	89587.0
37	11941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.57472535525606	16.773149104281725	11.31089984877051	27.341225691691704
2	18.054269401677516	19.076234319100884	36.16983005003231	26.69966622918929
3	19.737780057693715	22.585158191375257	28.4218170189803	29.255244731950725
4	13.879136326389213	15.758512488091512	34.95133341771983	35.41101776779945
5	14.679919788411958	34.80743222986882	33.32311812555378	17.189529856165432
6	34.10391531148611	33.67087932952706	17.46867151222827	14.75653384675856
7	29.151316096280556	29.431123961546408	22.094828217957	19.322731724216037
8	26.84889709066441	33.30646289547844	20.09753302732124	19.74710698653591
9	26.344576723982865	15.67723496532381	19.019606536844698	38.95858177384862
10	16.148911081057673	26.97081337481596	32.67622898942726	24.204046554699108
11	36.463628308561454	20.912306882607275	23.272019879682617	19.35204492914865
12	24.61643005136473	22.69774754668461	29.607003191142084	23.07881921080858
13	28.98476379552707	20.08953851688507	25.115420744422163	25.810276943165693
14	23.96221261400505	19.030265884092923	25.17338094508437	31.83414055681765
15	24.89956896264565	27.06608129084695	22.398619614531352	25.635730131976043
16	25.85557916897064	25.37923958881568	23.768345735927994	24.996835506285684
17	24.74034496312532	24.7490056827645	25.38057200722171	25.13007734688847
18	24.746340845952446	25.630400458351932	25.08943858550462	24.533820110191
19	25.500489663764213	24.81296176625384	25.076780610647354	24.60976795933459
20	25.86024263339174	24.886910987788387	24.603772076507465	24.649074302312414
21	24.8829137325703	24.889575824600442	24.964191255338	25.263319187491255
22	24.806965883426713	24.769658168057934	24.956196744901835	25.467179203613522
23	25.098765514346816	24.329293884865724	25.07078472782023	25.501155872967228
24	24.802968628208628	25.39123135446993	25.03814047687255	24.767659540448893
25	25.321945597356482	24.565131942732656	24.926217330766207	25.186705129144656
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	2.0
6	10.5
7	19.0
8	19.0
9	19.0
10	60.0
11	101.0
12	101.0
13	101.0
14	183.0
15	265.0
16	265.0
17	265.0
18	518.5
19	772.0
20	772.0
21	772.0
22	1212.0
23	1652.0
24	1652.0
25	1652.0
26	2364.0
27	3076.0
28	3076.0
29	3076.0
30	4157.0
31	5238.0
32	5238.0
33	5238.0
34	6700.5
35	8163.0
36	8163.0
37	8163.0
38	10269.5
39	12376.0
40	12376.0
41	12376.0
42	14767.5
43	17159.0
44	17159.0
45	17159.0
46	18764.5
47	20370.0
48	20370.0
49	20370.0
50	21150.5
51	21931.0
52	21931.0
53	21931.0
54	21426.5
55	20922.0
56	20922.0
57	20922.0
58	18672.5
59	16423.0
60	16423.0
61	16423.0
62	13873.0
63	11323.0
64	11323.0
65	11323.0
66	8846.0
67	6369.0
68	6369.0
69	6369.0
70	4516.0
71	2663.0
72	2663.0
73	2663.0
74	1812.0
75	961.0
76	961.0
77	961.0
78	607.0
79	253.0
80	253.0
81	253.0
82	154.0
83	55.0
84	55.0
85	55.0
86	32.0
87	9.0
88	9.0
89	9.0
90	4.5
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	150103.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.38816929660848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.753999973723	55.766405602007794
2	8.687999997241286	11.709368293260598
3	3.357999998933729	6.788684172784717
4	1.6890000309921425	4.552744801219019
5	1.0239999998979632	3.45027426764255
6	0.6159999998059663	2.4906667364181145
7	0.41599999986791925	1.9623434892941913
8	0.30999999990156535	1.6712265980252237
9	0.19499999993808137	1.1826623707799462
>10	0.9379999997021554	9.815760736627178
>50	0.009999999996824686	0.42993651997584376
>100	0.001999999999364937	0.17992641196481235
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGACA	50	0.0014885706	13.299999	4
GTCCTGG	105	0.0044638338	8.142858	1
>>END_MODULE