FastQCFastQC Report
Mon 15 Aug 2016
SRR2920835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150103
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC1600.10659347248222888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCT350.002157941516.28571316
CTAGAGG456.712121E-414.7777783
TCCCCCC400.005249759614.2515
ACTGTGT500.001488570613.2999996
TAGAGGT550.00304630112.0909094
CCTAGAG657.9495914E-411.6923072
CCCTAGA752.0501253E-411.4000011
AGAGGTG700.001477852510.8571425
GTGACAC700.001477852510.85714219
AGTGACA800.0044754639.518
GCCCAGT850.00736977538.94117614
ACACAGT850.00736977538.9411766
CAGTGAC850.00736977538.94117617
GAGGGCA850.00736977538.9411768
Produced by FastQC (version 0.11.5)