##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920835_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	150103
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06152441989833	33.0	33.0	34.0	32.0	34.0
2	33.08646063036715	33.0	33.0	34.0	32.0	35.0
3	33.28259928182648	34.0	33.0	34.0	32.0	35.0
4	33.3659154047554	34.0	33.0	34.0	32.0	35.0
5	33.248023024190054	34.0	33.0	34.0	32.0	35.0
6	35.93947489390619	37.0	37.0	37.0	33.0	37.0
7	35.95243932499684	37.0	37.0	37.0	33.0	37.0
8	35.90643757952872	37.0	36.0	37.0	33.0	37.0
9	35.88848990359953	37.0	35.0	37.0	33.0	37.0
10	35.88665116619921	37.0	35.0	37.0	33.0	37.0
11	35.78891161402504	37.0	35.0	37.0	33.0	37.0
12	35.7556144780584	37.0	35.0	37.0	33.0	37.0
13	37.51903026588409	38.0	38.0	38.0	37.0	38.0
14	37.537717434028636	38.0	38.0	38.0	37.0	38.0
15	37.541021831675586	38.0	38.0	38.0	37.0	38.0
16	37.50411384182861	38.0	38.0	38.0	37.0	38.0
17	37.437079871821346	38.0	38.0	38.0	37.0	38.0
18	37.47242893213327	38.0	38.0	38.0	37.0	38.0
19	37.46932439724722	38.0	38.0	38.0	37.0	38.0
20	37.47445420811043	38.0	38.0	38.0	37.0	38.0
21	37.45788558523147	38.0	38.0	38.0	37.0	38.0
22	37.469317735155194	38.0	38.0	38.0	37.0	38.0
23	38.14874452875692	39.0	38.0	39.0	37.0	39.0
24	38.14573992525133	39.0	38.0	39.0	37.0	39.0
25	38.01619554572527	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	1.0
19	0.0
20	0.0
21	0.0
22	1.0
23	8.0
24	34.0
25	66.0
26	128.0
27	239.0
28	416.0
29	669.0
30	1004.0
31	1409.0
32	2029.0
33	2971.0
34	5102.0
35	14608.0
36	90699.0
37	30718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61603032584292	17.077606710059094	11.342211681312167	26.96415128278582
2	18.268788765048	19.56723050172215	35.62487092196692	26.539109811262936
3	19.842374902566903	22.442589421930276	28.371851328754254	29.34318434674857
4	14.0410251627216	15.864439751370726	34.95799550974997	35.1365395761577
5	14.845805879962427	35.0978994423829	32.77682657908236	17.279468098572316
6	34.18585904345683	33.72417606576818	17.411377520769072	14.67858737000593
7	29.2492488491236	29.61166665556318	21.857657741684044	19.281426753629173
8	27.11005109824587	33.5822735055262	19.915657914898436	19.39201748132949
9	26.251307435560918	15.635929994736946	19.251447339493545	38.86131523020859
10	15.965703550228843	27.140030512381497	32.71087186798398	24.183394069405676
11	36.42698680239569	20.742423535838725	23.369286423322652	19.461303238442937
12	24.441217030972066	22.7403849356775	29.652305416947026	23.166092616403404
13	29.01207837285064	20.405321679113676	24.946203606856628	25.636396341179058
14	23.87427299920721	19.17216844433489	25.14540015855779	31.80815839790011
15	25.218016961686306	26.836239115807146	22.16011671985237	25.785627202654176
16	25.721671119164842	25.42454181462063	23.7830023383943	25.07078472782023
17	24.832948042344256	25.020819037594183	25.060125380572007	25.08610753948955
18	24.591780310853213	25.7483194872854	25.21335349726521	24.446546704596177
19	25.656382617269475	25.071450937023243	24.857597782855773	24.414568662851508
20	25.796286549902405	25.157391924212043	24.57579128998088	24.47053023590468
21	24.90822968228483	24.75566777479464	25.20336035922	25.132742183700525
22	25.08943858550462	24.91555798351798	24.984843740631433	25.01015969034596
23	25.230008727340557	24.439884612566036	25.00149897070678	25.328607689386622
24	24.848937063216592	25.280640626769618	25.140736694136695	24.7296856158771
25	25.104095187970927	24.42522801009973	25.346595337868	25.124081464061344
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	2.0
3	4.0
4	4.0
5	4.0
6	11.0
7	18.0
8	18.0
9	18.0
10	90.5
11	163.0
12	163.0
13	163.0
14	219.5
15	276.0
16	276.0
17	276.0
18	514.0
19	752.0
20	752.0
21	752.0
22	1207.0
23	1662.0
24	1662.0
25	1662.0
26	2391.5
27	3121.0
28	3121.0
29	3121.0
30	4203.0
31	5285.0
32	5285.0
33	5285.0
34	6719.5
35	8154.0
36	8154.0
37	8154.0
38	10395.0
39	12636.0
40	12636.0
41	12636.0
42	14752.0
43	16868.0
44	16868.0
45	16868.0
46	18670.0
47	20472.0
48	20472.0
49	20472.0
50	21174.5
51	21877.0
52	21877.0
53	21877.0
54	21296.5
55	20716.0
56	20716.0
57	20716.0
58	18662.5
59	16609.0
60	16609.0
61	16609.0
62	13945.5
63	11282.0
64	11282.0
65	11282.0
66	8819.5
67	6357.0
68	6357.0
69	6357.0
70	4498.0
71	2639.0
72	2639.0
73	2639.0
74	1777.0
75	915.0
76	915.0
77	915.0
78	577.0
79	239.0
80	239.0
81	239.0
82	144.0
83	49.0
84	49.0
85	49.0
86	28.5
87	8.0
88	8.0
89	8.0
90	4.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	150103.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.45769904665462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.09814044408802	54.56053509923186
2	8.99904766678362	11.961120030912108
3	3.453460979399529	6.885272113148972
4	1.7653250463635906	4.692777626030126
5	1.0235075936043307	3.400997981386115
6	0.7147511402937197	2.8500429704935946
7	0.40799959901759314	1.898030019386688
8	0.32279083755200244	1.7161549069638848
9	0.23758207608641171	1.4210242300287137
>10	0.9653651446042805	9.987142162381831
>50	0.010024560172422434	0.4443615384102916
>100	0.002004912034484487	0.18254132162581696
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCTAGAGGTGGAGCCCAGTGACAC	160	0.10659347248222888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCCCT	35	0.0021579415	16.285713	16
CTAGAGG	45	6.712121E-4	14.777778	3
TCCCCCC	40	0.0052497596	14.25	15
ACTGTGT	50	0.0014885706	13.299999	6
TAGAGGT	55	0.003046301	12.090909	4
CCTAGAG	65	7.9495914E-4	11.692307	2
CCCTAGA	75	2.0501253E-4	11.400001	1
AGAGGTG	70	0.0014778525	10.857142	5
GTGACAC	70	0.0014778525	10.857142	19
AGTGACA	80	0.004475463	9.5	18
GCCCAGT	85	0.0073697753	8.941176	14
ACACAGT	85	0.0073697753	8.941176	6
CAGTGAC	85	0.0073697753	8.941176	17
GAGGGCA	85	0.0073697753	8.941176	8
>>END_MODULE