##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920833_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90888
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02479975354282	33.0	32.0	34.0	32.0	34.0
2	32.19301778012499	33.0	32.0	34.0	32.0	34.0
3	32.357230877563595	33.0	32.0	34.0	32.0	34.0
4	32.53487809171728	33.0	32.0	34.0	32.0	34.0
5	32.59746061086172	33.0	32.0	34.0	32.0	34.0
6	35.42049555496875	37.0	35.0	37.0	33.0	37.0
7	35.499328844291874	37.0	35.0	37.0	33.0	37.0
8	35.47132734794472	37.0	35.0	37.0	33.0	37.0
9	35.52780345040049	37.0	35.0	37.0	33.0	37.0
10	35.52067379632074	37.0	35.0	37.0	33.0	37.0
11	35.478842091365195	37.0	34.0	37.0	33.0	37.0
12	35.48823827127894	37.0	34.0	37.0	33.0	37.0
13	37.097581638940234	38.0	38.0	38.0	37.0	38.0
14	37.090022885309395	38.0	38.0	38.0	37.0	38.0
15	37.029024733738225	38.0	38.0	38.0	37.0	38.0
16	37.05622304374615	38.0	38.0	38.0	37.0	38.0
17	37.0619333685415	38.0	38.0	38.0	37.0	38.0
18	37.06952512983012	38.0	38.0	38.0	37.0	38.0
19	37.042392835137754	38.0	38.0	38.0	37.0	38.0
20	37.07225376287299	38.0	38.0	38.0	37.0	38.0
21	37.09582123052548	38.0	38.0	38.0	37.0	38.0
22	37.13187659537012	38.0	38.0	38.0	37.0	38.0
23	37.708146289939265	39.0	38.0	39.0	37.0	39.0
24	37.6429121556201	39.0	38.0	39.0	37.0	39.0
25	37.546650822990934	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	14.0
20	23.0
21	52.0
22	107.0
23	160.0
24	275.0
25	419.0
26	458.0
27	587.0
28	694.0
29	856.0
30	1050.0
31	1357.0
32	1660.0
33	2395.0
34	4027.0
35	10987.0
36	55859.0
37	9903.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.253234750462106	17.929759704251385	11.658304726696594	27.158700818589914
2	16.960434820878444	20.783821846668427	37.28545022445207	24.97029310800106
3	18.997007305694922	23.916248569668163	29.735498635683477	27.351245488953435
4	13.398908546782854	17.041853710060735	36.99058181498108	32.56865592817534
5	14.039257107648975	35.389710412815774	34.51500748173576	16.05602499779949
6	31.95141272775284	35.293988205263624	18.794560337998416	13.960038728985126
7	27.169703371182113	30.61020156676349	23.120764017252	19.099331044802394
8	25.079218378663846	34.56451896840067	21.660725288266878	18.695537364668603
9	25.76247689463956	16.437813572748876	20.39103071912684	37.40867881348473
10	15.608221107296893	27.21591409206936	34.318061790335356	22.85780301029839
11	34.15302350145233	21.69263269078426	25.337778364580583	18.816565443182817
12	23.523457442126574	23.911847548631282	31.00629346008274	21.558401549159406
13	27.75613942434645	21.73334213537541	26.1090573012939	24.401461138984246
14	22.61134583223308	20.947759880292228	26.255391250770177	30.185503036704514
15	23.631282457530148	28.773875539125076	23.33641404805915	24.258427955285626
16	24.105492474254028	26.96945691400405	25.558929671683835	23.366120940058092
17	23.239591585247776	26.298301205879763	26.700994630754337	23.761112578118123
18	22.7653815685239	26.866032919637355	27.357847020508757	23.010738491329988
19	24.214417744916823	26.138764193292847	26.129962151219083	23.516855910571252
20	23.83372942522665	26.462239239503564	26.68559105712525	23.01844027814453
21	23.855734530411056	26.078250154035736	26.092553472405598	23.97346184314761
22	23.666490625825194	26.552460170759616	26.42923158172696	23.35181762168823
23	24.163806002992693	25.983628201742803	26.015535604260187	23.837030191004313
24	23.324311240207727	26.661385441422407	26.80221811460259	23.212085203767273
25	24.310139952468973	25.71846668427075	26.454537452689024	23.516855910571252
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	2.0
3	4.0
4	4.0
5	4.0
6	5.5
7	7.0
8	7.0
9	7.0
10	37.5
11	68.0
12	68.0
13	68.0
14	137.0
15	206.0
16	206.0
17	206.0
18	399.0
19	592.0
20	592.0
21	592.0
22	1103.0
23	1614.0
24	1614.0
25	1614.0
26	2410.0
27	3206.0
28	3206.0
29	3206.0
30	4304.5
31	5403.0
32	5403.0
33	5403.0
34	6336.0
35	7269.0
36	7269.0
37	7269.0
38	8345.5
39	9422.0
40	9422.0
41	9422.0
42	10341.5
43	11261.0
44	11261.0
45	11261.0
46	11743.0
47	12225.0
48	12225.0
49	12225.0
50	12127.5
51	12030.0
52	12030.0
53	12030.0
54	11080.5
55	10131.0
56	10131.0
57	10131.0
58	8877.5
59	7624.0
60	7624.0
61	7624.0
62	6415.0
63	5206.0
64	5206.0
65	5206.0
66	4061.5
67	2917.0
68	2917.0
69	2917.0
70	2056.0
71	1195.0
72	1195.0
73	1195.0
74	787.5
75	380.0
76	380.0
77	380.0
78	238.0
79	96.0
80	96.0
81	96.0
82	59.5
83	23.0
84	23.0
85	23.0
86	14.5
87	6.0
88	6.0
89	6.0
90	4.5
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	90888.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.6599771146906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.74912149930785	75.01320306311065
2	5.372164838675327	8.881260452424963
3	1.824885528697689	4.525349881172432
4	0.7666915131508891	2.5349881172432003
5	0.4033116813970823	1.6668867177185107
6	0.2529017144074113	1.2542909955109585
7	0.16105846022787776	0.9319162045594578
8	0.10648493238206794	0.704163365900889
9	0.07986369928655096	0.5941378399788751
>10	0.2781918858481525	3.6220403133526977
>50	0.0053242466191033965	0.27176304902737436
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACTGTG	40	0.0052286536	14.25	7
>>END_MODULE