##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920833_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90888
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05623404629874	33.0	33.0	34.0	32.0	34.0
2	33.11570284305959	33.0	33.0	34.0	32.0	35.0
3	33.27406258251914	34.0	33.0	34.0	32.0	35.0
4	33.356284658040664	34.0	33.0	34.0	32.0	35.0
5	33.25873602675821	34.0	33.0	34.0	32.0	35.0
6	36.04342707508142	37.0	37.0	37.0	33.0	37.0
7	36.048653287562715	37.0	37.0	37.0	34.0	37.0
8	36.01103556024998	37.0	37.0	37.0	33.0	37.0
9	35.994971833465364	37.0	37.0	37.0	33.0	37.0
10	35.98702799049379	37.0	37.0	37.0	33.0	37.0
11	35.89188891822903	37.0	35.0	37.0	33.0	37.0
12	35.863271278936715	37.0	35.0	37.0	33.0	37.0
13	37.527759440190124	38.0	38.0	38.0	37.0	38.0
14	37.546100695361325	38.0	38.0	38.0	37.0	38.0
15	37.54193072792888	38.0	38.0	38.0	37.0	38.0
16	37.51907842619488	38.0	38.0	38.0	37.0	38.0
17	37.45604480239415	38.0	38.0	38.0	37.0	38.0
18	37.504511046562804	38.0	38.0	38.0	37.0	38.0
19	37.49511486664906	38.0	38.0	38.0	37.0	38.0
20	37.486884957310096	38.0	38.0	38.0	37.0	38.0
21	37.47846800457706	38.0	38.0	38.0	37.0	38.0
22	37.48985564650999	38.0	38.0	38.0	37.0	38.0
23	38.19197253762873	39.0	38.0	39.0	37.0	39.0
24	38.187252442566674	39.0	38.0	39.0	37.0	39.0
25	38.05762036792536	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	4.0
24	14.0
25	29.0
26	65.0
27	140.0
28	249.0
29	393.0
30	619.0
31	858.0
32	1197.0
33	1731.0
34	2908.0
35	7960.0
36	54084.0
37	20635.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.03098318809964	18.077193908986885	11.804638676172873	27.0871842267406
2	17.15407974650119	20.898248393627323	37.21283337734354	24.734838482527945
3	19.23356218642725	23.54436229205176	29.97205351641581	27.250022005105183
4	13.440718246633217	17.40493794560338	36.510870521961095	32.6434732858023
5	14.027154299797553	35.34570020244697	34.282853622040314	16.344291875715168
6	31.632338702579	35.21476982659977	18.972801689992078	14.180089780829153
7	27.61640700642549	30.5287826775812	23.065751254290994	18.789059061702314
8	25.31247249361852	34.59422586039961	21.801557961447056	18.29174368453481
9	25.629346008273917	16.70187483496171	20.421837866385	37.24694129037937
10	15.289147082123053	27.89697209752663	33.88126045242496	22.932620367925356
11	34.497403397588236	21.97209752662618	25.100123228589034	18.43037584719655
12	23.55316433412552	23.727004665082298	31.17573277000264	21.544098230789544
13	28.02350145233694	21.737743156412286	26.138764193292847	24.099991197957927
14	22.527726432532347	21.152407358507176	26.50624944987237	29.813616759088106
15	23.850233254114954	28.545022445207284	23.290203327171906	24.314540973505853
16	23.96245929055541	27.26872634451193	25.224452072880908	23.54436229205176
17	23.01513951236687	26.752706627937684	26.81982219875011	23.41233166094534
18	23.089956869993838	26.65918493090397	27.351245488953435	22.89961271014875
19	24.350849397060117	25.9891294780389	26.315905290027285	23.34411583487369
20	23.94705571692633	26.59426987060998	26.66248569668163	22.796188715782062
21	23.721503388786196	26.581066807499337	26.275195845436137	23.422233958278323
22	23.520156676348915	26.551359915500395	26.56016195757416	23.368321450576534
23	23.819426106856792	25.991329988557343	26.25759176128862	23.931652143297242
24	23.326511750726166	26.479843323651085	26.796716838306487	23.396928087316258
25	24.182510342399436	25.816389402341343	26.40062494498724	23.600475310271985
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	2.0
6	7.0
7	12.0
8	12.0
9	12.0
10	72.0
11	132.0
12	132.0
13	132.0
14	178.5
15	225.0
16	225.0
17	225.0
18	443.5
19	662.0
20	662.0
21	662.0
22	1181.5
23	1701.0
24	1701.0
25	1701.0
26	2471.5
27	3242.0
28	3242.0
29	3242.0
30	4340.0
31	5438.0
32	5438.0
33	5438.0
34	6308.5
35	7179.0
36	7179.0
37	7179.0
38	8326.5
39	9474.0
40	9474.0
41	9474.0
42	10290.0
43	11106.0
44	11106.0
45	11106.0
46	11622.0
47	12138.0
48	12138.0
49	12138.0
50	12000.5
51	11863.0
52	11863.0
53	11863.0
54	11014.5
55	10166.0
56	10166.0
57	10166.0
58	9029.0
59	7892.0
60	7892.0
61	7892.0
62	6533.5
63	5175.0
64	5175.0
65	5175.0
66	4019.0
67	2863.0
68	2863.0
69	2863.0
70	1970.5
71	1078.0
72	1078.0
73	1078.0
74	735.5
75	393.0
76	393.0
77	393.0
78	254.5
79	116.0
80	116.0
81	116.0
82	71.0
83	26.0
84	26.0
85	26.0
86	15.0
87	4.0
88	4.0
89	4.0
90	2.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	90888.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.33870257899832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.48051739804373	74.50048411231406
2	5.550804425677482	9.140920693600915
3	1.9014912608904804	4.696989701610773
4	0.7603292533005506	2.5041809699850366
5	0.40622160457533807	1.6723879940146114
6	0.25255224758137795	1.2476894639556377
7	0.18440322839275214	1.0628465804066543
8	0.11892671976054306	0.7833817445647391
9	0.07349404030145919	0.5446263533139688
>10	0.2672510556416698	3.6352433764633396
>50	0.004008765834625046	0.2112490097702667
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATAAA	35	9.8092336E-5	19.0	1
CCCCCCT	35	9.8092336E-5	19.0	16
TCCCCCC	35	9.8092336E-5	19.0	15
GGGCATC	45	3.4800927E-5	16.88889	10
CATCCCC	45	3.4800927E-5	16.88889	13
GCATCCC	45	3.4800927E-5	16.88889	12
CCCCTGA	40	2.7253936E-4	16.625	18
GCATTTC	35	0.002149123	16.285715	13
GATAAAG	50	8.570951E-5	15.200001	2
GTGCATT	40	0.0052286536	14.25	11
AGTGTGC	40	0.0052286536	14.25	8
GTGTGCA	40	0.0052286536	14.25	9
ATCCCCC	55	1.9283855E-4	13.818182	14
GGCATCC	55	1.9283855E-4	13.818182	11
GAGGGCA	55	1.9283855E-4	13.818182	8
CCCTGAC	50	0.0014806837	13.300001	19
AGGGCAT	60	4.0265362E-4	12.666667	9
CCCCCTG	55	0.0030303902	12.090909	17
TTTCTCA	60	0.0058038225	11.083334	16
ATAAAGA	60	0.0058038225	11.083334	3
>>END_MODULE