##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920832_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102573
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.123882503192846	33.0	32.0	34.0	32.0	34.0
2	32.36796232926794	33.0	32.0	34.0	32.0	34.0
3	32.600889122868594	33.0	32.0	34.0	32.0	34.0
4	32.701812367777094	33.0	32.0	34.0	32.0	34.0
5	32.599836214208416	33.0	32.0	34.0	32.0	34.0
6	35.60176654675207	37.0	35.0	37.0	33.0	37.0
7	35.51448236865452	37.0	35.0	37.0	33.0	37.0
8	35.502237430902866	37.0	35.0	37.0	33.0	37.0
9	35.56410556384234	37.0	34.0	37.0	33.0	37.0
10	35.53463387051173	37.0	34.0	37.0	33.0	37.0
11	35.484415976914	37.0	34.0	37.0	33.0	37.0
12	35.4783130063467	37.0	34.0	37.0	33.0	37.0
13	37.16955729090501	38.0	38.0	38.0	37.0	38.0
14	37.19486609536623	38.0	38.0	38.0	37.0	38.0
15	37.139081434685544	38.0	38.0	38.0	37.0	38.0
16	37.17806830257475	38.0	38.0	38.0	37.0	38.0
17	37.174402620572664	38.0	38.0	38.0	37.0	38.0
18	37.19073245395962	38.0	38.0	38.0	37.0	38.0
19	37.15364667115128	38.0	38.0	38.0	37.0	38.0
20	37.18597486668032	38.0	38.0	38.0	37.0	38.0
21	37.22034063544987	38.0	38.0	38.0	37.0	38.0
22	37.24242246985074	38.0	38.0	38.0	37.0	38.0
23	37.8388854766849	39.0	38.0	39.0	37.0	39.0
24	37.72703342984996	39.0	38.0	39.0	37.0	39.0
25	37.6555233833465	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	8.0
20	28.0
21	43.0
22	102.0
23	149.0
24	226.0
25	316.0
26	416.0
27	546.0
28	654.0
29	853.0
30	1067.0
31	1294.0
32	1723.0
33	2561.0
34	4389.0
35	12994.0
36	64619.0
37	10582.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.644038879627196	17.53775359987521	11.837423103545767	26.98078441695183
2	17.583574624901292	19.8921743538748	36.713365115576224	25.810885905647684
3	19.647470581927017	22.705780273561267	29.250387528881873	28.396361615629846
4	14.059255359597556	16.156298441110234	35.52201846489817	34.26242773439404
5	14.821639222797423	34.51298099889834	33.533190995681124	17.132188782623107
6	33.029159720394254	34.04989617150712	18.21434490557944	14.706599202519183
7	28.390512123073325	29.49704113168183	22.603414153822158	19.509032591422695
8	26.07021340898677	33.834439862341945	20.938258606065922	19.157088122605366
9	26.08191239409981	15.99348756495374	19.630897019683545	38.29370302126291
10	16.113402162362416	26.763378276934475	33.10617803905511	24.017041521647997
11	34.93219463211567	20.984079631092005	24.822321663595684	19.26140407319665
12	24.336813781404462	22.831544363526465	30.150234467159976	22.6814073879091
13	28.63131623331676	20.753024675109437	25.435543466604273	25.180115624969535
14	23.449640743665487	20.078383200257377	25.22983631169996	31.242139744377173
15	24.881791504586978	27.873806947247324	22.236845953613525	25.007555594552173
16	24.969533892934788	25.996119836604176	24.342663273960984	24.691682996500052
17	23.792810973648034	25.744591656673784	26.021467637682434	24.44112973199575
18	24.07651136263929	25.684146900256405	26.375361937351933	23.86397979975237
19	25.157692570169537	25.234710888830392	25.399471595839064	24.208124945161007
20	24.847669464673938	25.673422830569447	25.36924921763037	24.109658487126243
21	24.26174529359578	25.279556998430387	25.35462548623907	25.104072221734764
22	24.397258537821845	25.21423766488257	25.600304173612937	24.788199623682647
23	24.754077583769607	25.00170610199565	25.384847864447757	24.85936844978698
24	24.178877482378404	25.63442621352598	25.81576048277812	24.370935821317502
25	24.97830813176957	24.982207793473915	25.668548253439017	24.370935821317502
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	3.0
4	3.0
5	3.0
6	8.0
7	13.0
8	13.0
9	13.0
10	46.5
11	80.0
12	80.0
13	80.0
14	141.5
15	203.0
16	203.0
17	203.0
18	399.0
19	595.0
20	595.0
21	595.0
22	1031.5
23	1468.0
24	1468.0
25	1468.0
26	2174.0
27	2880.0
28	2880.0
29	2880.0
30	3781.0
31	4682.0
32	4682.0
33	4682.0
34	5671.5
35	6661.0
36	6661.0
37	6661.0
38	7924.5
39	9188.0
40	9188.0
41	9188.0
42	10416.0
43	11644.0
44	11644.0
45	11644.0
46	12688.5
47	13733.0
48	13733.0
49	13733.0
50	14061.5
51	14390.0
52	14390.0
53	14390.0
54	13618.0
55	12846.0
56	12846.0
57	12846.0
58	11619.5
59	10393.0
60	10393.0
61	10393.0
62	8743.0
63	7093.0
64	7093.0
65	7093.0
66	5553.5
67	4014.0
68	4014.0
69	4014.0
70	2870.0
71	1726.0
72	1726.0
73	1726.0
74	1215.5
75	705.0
76	705.0
77	705.0
78	451.0
79	197.0
80	197.0
81	197.0
82	124.5
83	52.0
84	52.0
85	52.0
86	28.0
87	4.0
88	4.0
89	4.0
90	3.5
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	102573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.59043802950094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.98987125425533	57.75301492595517
2	8.912735882097476	12.404823881528278
3	3.3384233899777254	6.969670381094439
4	1.6573037643070283	4.613299796242676
5	0.9218139280760987	3.207471751825529
6	0.6164105294125888	2.5737767248691177
7	0.36284165254059203	1.7675216674953447
8	0.24796514478642775	1.3804802433388905
9	0.21013995320883708	1.3161358252171624
>10	0.7326879701881452	7.6345627016856294
>50	0.00980653114974573	0.3792421007477601
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAGT	35	0.0021516688	16.285715	6
TATGGTG	45	6.686463E-4	14.777779	5
CTATGGT	55	1.9322138E-4	13.818182	4
>>END_MODULE