##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920832_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102573
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86994628216002	33.0	32.0	34.0	32.0	34.0
2	32.8984528092188	33.0	32.0	34.0	32.0	34.0
3	33.107347937566416	33.0	33.0	34.0	32.0	34.0
4	33.19292601366831	33.0	33.0	34.0	32.0	35.0
5	33.06511460130834	33.0	33.0	34.0	32.0	35.0
6	35.84780595283359	37.0	35.0	37.0	33.0	37.0
7	35.87978317880924	37.0	37.0	37.0	33.0	37.0
8	35.82423249783081	37.0	35.0	37.0	33.0	37.0
9	35.82584110828385	37.0	35.0	37.0	33.0	37.0
10	35.814268862176206	37.0	35.0	37.0	33.0	37.0
11	35.728642040303	37.0	35.0	37.0	33.0	37.0
12	35.69412028506527	37.0	35.0	37.0	33.0	37.0
13	37.43484152749749	38.0	38.0	38.0	37.0	38.0
14	37.461164244001836	38.0	38.0	38.0	37.0	38.0
15	37.46176869156601	38.0	38.0	38.0	37.0	38.0
16	37.438789934973144	38.0	38.0	38.0	37.0	38.0
17	37.35576613728759	38.0	38.0	38.0	37.0	38.0
18	37.38016827040254	38.0	38.0	38.0	37.0	38.0
19	37.407514648104275	38.0	38.0	38.0	37.0	38.0
20	37.40056350111628	38.0	38.0	38.0	37.0	38.0
21	37.38554980355454	38.0	38.0	38.0	37.0	38.0
22	37.38563754594289	38.0	38.0	38.0	37.0	38.0
23	38.056018640382945	39.0	38.0	39.0	37.0	39.0
24	38.04107318690104	39.0	38.0	39.0	37.0	39.0
25	37.913983211956364	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0
22	0.0
23	7.0
24	24.0
25	54.0
26	109.0
27	192.0
28	349.0
29	550.0
30	823.0
31	1126.0
32	1599.0
33	2349.0
34	3943.0
35	11326.0
36	62568.0
37	17552.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.14585709689685	17.903346884657758	12.082126875493552	26.868669142951852
2	17.640119719614322	20.10470591676172	36.53008101547191	25.72509334815205
3	19.63479668138789	22.958283368917744	29.132422762325366	28.274497187368997
4	14.274711668762736	16.579411736031897	35.152525518411274	33.993351076794085
5	14.753395142971348	34.99751396566348	33.37817944293332	16.87091144843185
6	33.307985532255074	33.931931404950625	18.1139286166925	14.6461544461018
7	28.642040303003714	29.615980813664418	22.548818889961293	19.193159993370575
8	25.95907305041288	34.57147592446355	20.815419262378988	18.654031762744584
9	26.017567975978086	15.948641455353748	19.809306542657424	38.22448402601074
10	16.104627923527634	27.00320747175183	32.89852105329862	23.993643551421915
11	35.21979468281127	21.201485771109358	24.426506000604448	19.15221354547493
12	24.415781930917493	22.71650434324822	30.240901601786046	22.62681212404824
13	28.47045518801244	20.5785148138399	25.792362512552035	25.158667485595625
14	23.739190625213265	20.137853041248672	25.14696850048258	30.975987833055484
15	24.45380363253488	28.083413763855987	22.464001247891748	24.99878135571739
16	25.280531913856475	26.0458405233346	24.242246985074047	24.43138057773488
17	23.99169372056974	25.516461446969473	26.207676484065008	24.284168348395777
18	23.948797441821924	25.97272186637809	26.215475807473705	23.863004884326287
19	25.240560381386917	25.258108859056478	25.35657531709124	24.144755442465364
20	24.794049116239165	25.85865676152594	25.523285854952082	23.82400826728281
21	24.68875825022179	25.09919764460433	24.989032201456524	25.223011903717353
22	24.645861971473977	25.325378023456462	25.5661821336999	24.46257787136966
23	24.61953925496963	25.01730474881304	25.45504177512601	24.90811422109132
24	24.21397443771753	25.75141606465639	25.562282471995555	24.472327025630527
25	24.99975627114348	24.92956236046523	25.6032289198912	24.467452448500094
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	0.5
3	0.0
4	0.0
5	0.0
6	8.5
7	17.0
8	17.0
9	17.0
10	80.0
11	143.0
12	143.0
13	143.0
14	185.0
15	227.0
16	227.0
17	227.0
18	407.0
19	587.0
20	587.0
21	587.0
22	1024.0
23	1461.0
24	1461.0
25	1461.0
26	2184.0
27	2907.0
28	2907.0
29	2907.0
30	3845.5
31	4784.0
32	4784.0
33	4784.0
34	5710.5
35	6637.0
36	6637.0
37	6637.0
38	7889.5
39	9142.0
40	9142.0
41	9142.0
42	10403.0
43	11664.0
44	11664.0
45	11664.0
46	12713.0
47	13762.0
48	13762.0
49	13762.0
50	14044.0
51	14326.0
52	14326.0
53	14326.0
54	13627.5
55	12929.0
56	12929.0
57	12929.0
58	11643.0
59	10357.0
60	10357.0
61	10357.0
62	8708.5
63	7060.0
64	7060.0
65	7060.0
66	5495.0
67	3930.0
68	3930.0
69	3930.0
70	2819.5
71	1709.0
72	1709.0
73	1709.0
74	1184.0
75	659.0
76	659.0
77	659.0
78	436.0
79	213.0
80	213.0
81	213.0
82	131.0
83	49.0
84	49.0
85	49.0
86	29.0
87	9.0
88	9.0
89	9.0
90	4.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	102573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.3564583272401
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.0154200812471	57.57655523383346
2	8.706652984917277	12.077252298363117
3	3.4115348392628726	7.098359217337896
4	1.7078759084072477	4.738088970781785
5	0.9235180838054006	3.202597174695095
6	0.5777259245723282	2.4041414407300166
7	0.4441883021042718	2.156512922503973
8	0.25723563064899285	1.4272761837910561
9	0.21084887758114168	1.3161358252171624
>10	0.7365654123501216	7.650161348503018
>50	0.008433955103245667	0.3529193842434169
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGATC	25	0.005994581	19.0	3
AAGTGTG	55	0.0030349838	12.090909	7
>>END_MODULE