FastQCFastQC Report
Mon 15 Aug 2016
SRR2920831_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920831_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181530
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC1910.10521676857819644No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATC307.686874E-418.9999981
GAACTCA402.7442977E-416.6256
CCCCCCT400.005255369514.2516
GGACCAT400.005255369514.256
TCCCCCC400.005255369514.2515
TCTAGGG500.00149066713.3000013
CTAGAGG550.003050532212.0909093
CCCCCTG600.005841846611.08333317
AAGGGTG750.002628357810.1333335
AGTGACA856.533386E-410.05882418
GTGACAC856.533386E-410.05882419
GAGCCCA951.6257411E-410.012
AGAGGTG900.00110357059.55
GAGGTGG1054.4430757E-49.047626
CCCAGTG1107.066228E-48.63636415
GCCCAGT1000.00287907858.5514
AGCCCAG1000.00287907858.5513
CAGTGAC1050.00447300778.14285817
CCAGTGA1050.00447300778.14285816
GGTGGAG1400.00753428556.7857148
Produced by FastQC (version 0.11.5)