##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920831_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	181530
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.812207348647608	33.0	32.0	33.0	30.0	34.0
2	32.19086652343965	33.0	32.0	34.0	32.0	34.0
3	32.50865421693384	33.0	32.0	34.0	32.0	34.0
4	32.61255990745332	33.0	32.0	34.0	32.0	34.0
5	32.791400870379555	33.0	33.0	34.0	32.0	34.0
6	35.55226684294607	37.0	35.0	37.0	33.0	37.0
7	35.481600837327164	37.0	35.0	37.0	33.0	37.0
8	35.52923483721699	37.0	35.0	37.0	33.0	37.0
9	35.56856167024734	37.0	34.0	37.0	33.0	37.0
10	35.5435520299675	37.0	34.0	37.0	33.0	37.0
11	35.46636919517435	37.0	34.0	37.0	33.0	37.0
12	35.4724508345728	37.0	34.0	37.0	33.0	37.0
13	37.21946785655263	38.0	38.0	38.0	37.0	38.0
14	37.20596595604032	38.0	38.0	38.0	37.0	38.0
15	37.15054261003691	38.0	38.0	38.0	37.0	38.0
16	37.18149617143172	38.0	38.0	38.0	37.0	38.0
17	37.18039442516388	38.0	38.0	38.0	37.0	38.0
18	37.18491709359335	38.0	38.0	38.0	37.0	38.0
19	37.167933674874675	38.0	38.0	38.0	37.0	38.0
20	37.18635487247287	38.0	38.0	38.0	37.0	38.0
21	37.21695587506197	38.0	38.0	38.0	37.0	38.0
22	37.23150443452873	38.0	38.0	38.0	37.0	38.0
23	37.83642373161461	39.0	38.0	39.0	37.0	39.0
24	37.75352834242274	39.0	38.0	39.0	37.0	39.0
25	37.67263262270699	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	17.0
20	49.0
21	92.0
22	205.0
23	290.0
24	426.0
25	581.0
26	814.0
27	994.0
28	1171.0
29	1541.0
30	1829.0
31	2225.0
32	3029.0
33	4329.0
34	7678.0
35	23493.0
36	115176.0
37	17584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.64551313832425	16.936594502286123	11.666942103233625	26.75095025615601
2	18.16944857599295	19.096017187241777	36.21219633118493	26.522337905580347
3	19.583539910758553	22.529058557814135	28.471327053379607	29.416074478047705
4	14.210874235663526	15.648102242053655	35.257533190106315	34.8834903321765
5	15.198589764777171	34.458767145926295	33.18514846030959	17.157494628986942
6	34.03955269101526	33.889164325455845	17.334324904974384	14.73695807855451
7	29.35658017958464	29.629813254007605	21.825593565801796	19.18801300060596
8	27.196606621495068	33.174130997631245	20.061146917864818	19.56811546300887
9	26.69035421142511	15.903707376191264	19.288271910978903	38.11766650140473
10	16.465597972786867	26.410510659395143	32.61334214730348	24.510549220514516
11	36.045832644741914	21.130942543932132	23.300831818432215	19.522392992893735
12	24.765603481518205	22.845259736682642	29.12686608274114	23.262270699058007
13	28.771552911364513	20.375144604197654	25.07574505591362	25.77755742852421
14	24.22189169834187	19.20784443342698	24.946289869443067	31.62397399878808
15	25.10273783947557	26.77904478598579	22.167685781964412	25.950531592574226
16	25.76984520464937	25.374869167630692	23.7024183330579	25.15286729466204
17	24.872472869498154	24.859802787418058	25.1611303916708	25.106593951412993
18	24.810224205365504	25.4420756899686	25.342918525863496	24.4047815788024
19	25.49055252575332	24.860353660551976	25.09447474246681	24.554619071227897
20	25.82438164490718	24.842174847132707	24.57445050404892	24.7589930039112
21	25.06968545144053	24.792596265080153	24.696744339778547	25.44097394370077
22	25.048752272351678	24.5133035861841	25.155621660331622	25.282322481132596
23	25.325841458712055	24.210874235663525	24.953451220183993	25.509833085440427
24	24.96942654106759	25.18702142896491	24.770561339723464	25.072990690244033
25	25.226684294606954	24.741915936759764	25.001377182834794	25.030022585798488
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.5
3	2.0
4	2.0
5	2.0
6	15.5
7	29.0
8	29.0
9	29.0
10	77.0
11	125.0
12	125.0
13	125.0
14	270.5
15	416.0
16	416.0
17	416.0
18	726.5
19	1037.0
20	1037.0
21	1037.0
22	1545.5
23	2054.0
24	2054.0
25	2054.0
26	2978.0
27	3902.0
28	3902.0
29	3902.0
30	5088.5
31	6275.0
32	6275.0
33	6275.0
34	8003.0
35	9731.0
36	9731.0
37	9731.0
38	12313.5
39	14896.0
40	14896.0
41	14896.0
42	17460.5
43	20025.0
44	20025.0
45	20025.0
46	22218.5
47	24412.0
48	24412.0
49	24412.0
50	25407.0
51	26402.0
52	26402.0
53	26402.0
54	25829.5
55	25257.0
56	25257.0
57	25257.0
58	22661.0
59	20065.0
60	20065.0
61	20065.0
62	17081.0
63	14097.0
64	14097.0
65	14097.0
66	11014.0
67	7931.0
68	7931.0
69	7931.0
70	5605.5
71	3280.0
72	3280.0
73	3280.0
74	2236.0
75	1192.0
76	1192.0
77	1192.0
78	758.0
79	324.0
80	324.0
81	324.0
82	195.5
83	67.0
84	67.0
85	67.0
86	37.0
87	7.0
88	7.0
89	7.0
90	5.0
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	181530.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.614052127782685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.98165880418392	51.51571464563668
2	9.469060496819004	12.047306160915438
3	3.565373568262283	6.804235800793662
4	1.8720226788779912	4.763477931141437
5	1.1287900682673575	3.5903455122041525
6	0.7417990698597264	2.831330681903841
7	0.5034954717334004	2.242057102946575
8	0.33001267024135844	1.6794754566050025
9	0.2502511445982741	1.43275404217607
>10	1.122640453703705	11.448077562372937
>50	0.030907507915513905	1.2538974669361729
>100	0.003988065537485666	0.39132763636804185
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCTAGAGGTGGAGCCCAGTGACAC	191	0.10521676857819644	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATC	30	7.686874E-4	18.999998	1
GAACTCA	40	2.7442977E-4	16.625	6
CCCCCCT	40	0.0052553695	14.25	16
GGACCAT	40	0.0052553695	14.25	6
TCCCCCC	40	0.0052553695	14.25	15
TCTAGGG	50	0.001490667	13.300001	3
CTAGAGG	55	0.0030505322	12.090909	3
CCCCCTG	60	0.0058418466	11.083333	17
AAGGGTG	75	0.0026283578	10.133333	5
AGTGACA	85	6.533386E-4	10.058824	18
GTGACAC	85	6.533386E-4	10.058824	19
GAGCCCA	95	1.6257411E-4	10.0	12
AGAGGTG	90	0.0011035705	9.5	5
GAGGTGG	105	4.4430757E-4	9.04762	6
CCCAGTG	110	7.066228E-4	8.636364	15
GCCCAGT	100	0.0028790785	8.55	14
AGCCCAG	100	0.0028790785	8.55	13
CAGTGAC	105	0.0044730077	8.142858	17
CCAGTGA	105	0.0044730077	8.142858	16
GGTGGAG	140	0.0075342855	6.785714	8
>>END_MODULE