FastQCFastQC Report
Mon 15 Aug 2016
SRR2920831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181530
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC2440.13441304467581117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGTC500.00149066713.3000017
TGGAGGT604.0611727E-412.6666664
AGCCCAG751.4616286E-512.66666613
CCAGTGA802.8338289E-511.87516
GAGCCCA907.359453E-611.61111112
AGGTGGA1001.898683E-611.4000017
CAGTGAC855.2628428E-511.17647117
GCCCAGT951.34184575E-511.014
GTGACAC951.34184575E-511.019
AGTGACA1002.366515E-510.45000118
CCCAGTG1054.0502127E-59.95238115
GGAGCCC950.00180736539.011
GTGGAGC950.00180736539.09
AGCACTG950.00180736539.05
GGTGGAG1201.7406521E-48.7083338
GAGGTGG1107.066228E-48.6363646
Produced by FastQC (version 0.11.5)