##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920830_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	122061
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.209673851598794	33.0	32.0	34.0	32.0	34.0
2	32.42965402544629	33.0	32.0	34.0	32.0	34.0
3	32.55741801230532	33.0	32.0	34.0	32.0	34.0
4	32.65952269766756	33.0	32.0	34.0	32.0	34.0
5	32.617592842922804	33.0	32.0	34.0	32.0	34.0
6	35.60854818492393	37.0	35.0	37.0	33.0	37.0
7	35.49800509581275	37.0	35.0	37.0	33.0	37.0
8	35.49961085031255	37.0	35.0	37.0	33.0	37.0
9	35.595808653050526	37.0	35.0	37.0	33.0	37.0
10	35.572205700428476	37.0	35.0	37.0	33.0	37.0
11	35.57072283530366	37.0	34.0	37.0	33.0	37.0
12	35.60231359729971	37.0	35.0	37.0	33.0	37.0
13	37.21336872547333	38.0	38.0	38.0	37.0	38.0
14	37.21309017622336	38.0	38.0	38.0	37.0	38.0
15	37.17298727685338	38.0	38.0	38.0	37.0	38.0
16	37.19115851910111	38.0	38.0	38.0	37.0	38.0
17	37.19976077535003	38.0	38.0	38.0	37.0	38.0
18	37.22256085072218	38.0	38.0	38.0	37.0	38.0
19	37.19673769672541	38.0	38.0	38.0	37.0	38.0
20	37.212918131098384	38.0	38.0	38.0	37.0	38.0
21	37.23076986097115	38.0	38.0	38.0	37.0	38.0
22	37.25069432496866	38.0	38.0	38.0	37.0	38.0
23	37.88219005251472	39.0	38.0	39.0	37.0	39.0
24	37.79892021202514	39.0	38.0	39.0	37.0	39.0
25	37.72712004653411	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	1.0
18	3.0
19	11.0
20	28.0
21	62.0
22	112.0
23	168.0
24	277.0
25	408.0
26	519.0
27	629.0
28	793.0
29	963.0
30	1280.0
31	1509.0
32	2077.0
33	2915.0
34	4995.0
35	14252.0
36	76802.0
37	14255.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.44107454469487	18.150760685231155	12.399537935950057	27.008626834123923
2	16.606450872924196	20.484020284939497	38.12110338273486	24.78842545940145
3	18.75455714765568	23.97735558450283	29.994019383750746	27.27406788409074
4	13.302365210837205	17.463399447817075	36.99297892037588	32.24125642096985
5	13.848813298268897	35.4888129705639	33.86421543326698	16.79815829790023
6	31.589123471051362	35.02183334562227	19.086358460114205	14.302684723212163
7	27.238020334095246	31.01318193362335	22.77713602215286	18.971661710128544
8	24.899845159387517	35.12014484560998	21.492532422313435	18.487477572689066
9	25.727300284284087	16.887457910389067	20.61510228492311	36.77013952040373
10	15.827332235521583	27.61078477154865	33.852745758268405	22.70913723466136
11	33.84537239576933	21.515471772310566	25.62816952179648	19.010986310123627
12	23.8167801345229	23.769262909528845	30.97959217112755	21.4343647848207
13	27.732035621533495	21.857104234767863	26.275386896715574	24.135473246983068
14	22.72552248465931	21.046034359869246	26.452347596693453	29.77609555877799
15	23.59394073455076	28.607007971424125	23.19168284710104	24.60736844692408
16	23.729119047033862	26.834943184145633	26.105799559236775	23.330138209583733
17	23.425172659571853	26.286037309214244	26.765305871654338	23.523484159559565
18	23.403871834574517	26.800534159149937	27.446113009069233	22.349480997206314
19	24.670451659416194	25.91409213426074	26.111534396736058	23.30392180958701
20	24.101883484487267	26.68583740916427	26.472009896691002	22.740269209657466
21	23.90034490951246	26.16314793422961	26.051728234243534	23.8847789220144
22	23.684878872039388	26.725162009159355	26.438420134195194	23.151538984606056
23	23.479243984565095	26.286856571714146	26.357313146705337	23.876586297015425
24	23.176116859602985	26.691572246663554	26.883279671639592	23.249031222093873
25	24.07320929699085	25.870671221766166	26.461359484192332	23.594759997050655
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	2.0
3	4.0
4	4.0
5	4.0
6	9.5
7	15.0
8	15.0
9	15.0
10	54.0
11	93.0
12	93.0
13	93.0
14	199.0
15	305.0
16	305.0
17	305.0
18	563.5
19	822.0
20	822.0
21	822.0
22	1490.5
23	2159.0
24	2159.0
25	2159.0
26	3348.5
27	4538.0
28	4538.0
29	4538.0
30	6037.5
31	7537.0
32	7537.0
33	7537.0
34	8728.0
35	9919.0
36	9919.0
37	9919.0
38	11495.0
39	13071.0
40	13071.0
41	13071.0
42	14176.5
43	15282.0
44	15282.0
45	15282.0
46	15622.5
47	15963.0
48	15963.0
49	15963.0
50	15827.5
51	15692.0
52	15692.0
53	15692.0
54	14506.0
55	13320.0
56	13320.0
57	13320.0
58	11744.0
59	10168.0
60	10168.0
61	10168.0
62	8552.0
63	6936.0
64	6936.0
65	6936.0
66	5413.0
67	3890.0
68	3890.0
69	3890.0
70	2749.0
71	1608.0
72	1608.0
73	1608.0
74	1076.5
75	545.0
76	545.0
77	545.0
78	344.0
79	143.0
80	143.0
81	143.0
82	93.0
83	43.0
84	43.0
85	43.0
86	24.5
87	6.0
88	6.0
89	6.0
90	4.0
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	122061.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.48534749018933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1847599164927	69.99696872875037
2	6.077244258872652	9.539492548807564
3	2.059498956158664	4.849214736893848
4	0.9791231732776619	3.073872899615766
5	0.5	1.9621336872547332
6	0.29436325678496866	1.386192149826726
7	0.21398747390396658	1.1756416873530449
8	0.13674321503131523	0.8585870998926767
9	0.10438413361169101	0.737336249907833
>10	0.4321503131524009	5.439083736820114
>50	0.017745302713987474	0.9814764748773155
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGACAC	55	1.1114154E-5	15.545455	19
GAGCCCA	50	8.6095875E-5	15.2	12
ACTCATT	40	0.005242314	14.250001	14
ATTGATA	40	0.005242314	14.250001	18
GGAGCCC	50	0.0014857874	13.299999	11
GTGGAGC	50	0.0014857874	13.299999	9
TCATTGA	55	0.0030406874	12.090909	16
CCAGGAC	55	0.0030406874	12.090909	3
AGCCCAG	65	7.930894E-4	11.692307	13
GAGGTGG	65	7.930894E-4	11.692307	6
GGTGGAG	65	7.930894E-4	11.692307	8
CACAGTG	65	7.930894E-4	11.692307	7
CAGTGAC	65	7.930894E-4	11.692307	17
CCAGTGA	65	7.930894E-4	11.692307	16
GCCCAGT	70	0.0014744205	10.857142	14
AGGTGGA	75	0.0026178064	10.133334	7
AGTGACA	80	0.0044653346	9.500001	18
CCCAGTG	85	0.0073533114	8.941176	15
TTCTCAT	190	0.003320208	6.0	17
>>END_MODULE