##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920829_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92026
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.059787451372436	33.0	32.0	34.0	32.0	34.0
2	32.18346988894443	33.0	32.0	34.0	31.0	34.0
3	32.40244061460891	33.0	32.0	34.0	32.0	34.0
4	32.62068328515854	33.0	32.0	34.0	32.0	34.0
5	32.641373090213634	33.0	32.0	34.0	32.0	34.0
6	35.49900028252885	37.0	35.0	37.0	33.0	37.0
7	35.60473127159716	37.0	37.0	37.0	33.0	37.0
8	35.52950253189316	37.0	35.0	37.0	33.0	37.0
9	35.62827896464043	37.0	35.0	37.0	33.0	37.0
10	35.60077586768956	37.0	35.0	37.0	33.0	37.0
11	35.562569273900856	37.0	34.0	37.0	33.0	37.0
12	35.5789559472323	37.0	35.0	37.0	33.0	37.0
13	37.147469193488796	38.0	38.0	38.0	37.0	38.0
14	37.16361680394671	38.0	38.0	38.0	37.0	38.0
15	37.10253623975833	38.0	38.0	38.0	37.0	38.0
16	37.1445026405581	38.0	38.0	38.0	37.0	38.0
17	37.149990220155175	38.0	38.0	38.0	37.0	38.0
18	37.152217851476756	38.0	38.0	38.0	37.0	38.0
19	37.13031099906548	38.0	38.0	38.0	37.0	38.0
20	37.14174255101819	38.0	38.0	38.0	37.0	38.0
21	37.17503748940517	38.0	38.0	38.0	37.0	38.0
22	37.19806359072436	38.0	38.0	38.0	37.0	38.0
23	37.81142285875731	39.0	38.0	39.0	37.0	39.0
24	37.72441483928455	39.0	38.0	39.0	37.0	39.0
25	37.652185251994	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	9.0
20	27.0
21	48.0
22	101.0
23	163.0
24	260.0
25	288.0
26	409.0
27	512.0
28	671.0
29	765.0
30	962.0
31	1215.0
32	1623.0
33	2227.0
34	3970.0
35	11275.0
36	56727.0
37	10770.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.76291482841806	18.328515854215112	11.983569860691544	26.924999456675287
2	16.40514637167757	20.662638819464068	38.251146415143545	24.68106839371482
3	18.564318779475368	24.038858583443808	30.352291743637664	27.04453089344316
4	13.12455175711212	17.47658270488775	36.80372938082716	32.59513615717297
5	13.308195509964577	35.70947340968856	34.6608567144068	16.321474365940063
6	31.20313824353987	35.5464759959142	19.19457544606959	14.055810314476345
7	26.683763284289224	30.612000956251496	23.57485927889944	19.12937648055984
8	24.665855301762544	35.33783930628301	22.016604003216482	17.979701388737965
9	25.459109382131135	16.624649555560385	20.521374394192947	37.39486666811553
10	15.411948797079086	27.900810640471168	34.12948514550236	22.557755416947384
11	34.0675461282681	21.741681698650382	25.511268554538937	18.679503618542586
12	23.56073283637233	23.738943342098974	31.352009214787124	21.34831460674157
13	27.854084715189188	21.972051376784822	26.24367026709843	23.930193640927563
14	22.35998522156782	21.185317192967204	26.556625301545218	29.898072283919763
15	23.357529393866947	29.09612500814987	23.49010062373677	24.056244974246408
16	23.738943342098974	26.731575858996372	26.389281290070198	23.14019950883446
17	23.03805446286919	26.583791537174278	27.003238215286984	23.37491578466955
18	22.90222328472388	26.944559146328213	27.747593071523262	22.405624497424643
19	24.324647382261535	26.255623410775215	26.31538912915915	23.104340077804096
20	23.753069784626085	27.011931410688284	26.59031143372525	22.64468737096038
21	23.567252732923304	26.489253037185144	26.033946928042074	23.909547301849475
22	23.211918370895184	26.859800491165544	26.901093169321715	23.027187968617564
23	23.18583878469128	26.350161910764346	26.731575858996372	23.732423445547997
24	23.08586703757634	26.643557255558214	27.170582226762	23.099993480103446
25	23.824788646686805	25.660139525786192	27.08691022102449	23.42816160650251
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	6.5
7	12.0
8	12.0
9	12.0
10	52.0
11	92.0
12	92.0
13	92.0
14	184.0
15	276.0
16	276.0
17	276.0
18	509.0
19	742.0
20	742.0
21	742.0
22	1233.0
23	1724.0
24	1724.0
25	1724.0
26	2613.0
27	3502.0
28	3502.0
29	3502.0
30	4775.5
31	6049.0
32	6049.0
33	6049.0
34	7030.5
35	8012.0
36	8012.0
37	8012.0
38	9021.5
39	10031.0
40	10031.0
41	10031.0
42	10683.5
43	11336.0
44	11336.0
45	11336.0
46	11578.0
47	11820.0
48	11820.0
49	11820.0
50	11668.0
51	11516.0
52	11516.0
53	11516.0
54	10659.5
55	9803.0
56	9803.0
57	9803.0
58	8634.5
59	7466.0
60	7466.0
61	7466.0
62	6272.5
63	5079.0
64	5079.0
65	5079.0
66	3938.5
67	2798.0
68	2798.0
69	2798.0
70	1998.0
71	1198.0
72	1198.0
73	1198.0
74	813.0
75	428.0
76	428.0
77	428.0
78	268.5
79	109.0
80	109.0
81	109.0
82	68.5
83	28.0
84	28.0
85	28.0
86	15.5
87	3.0
88	3.0
89	3.0
90	2.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	92026.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.0501597374655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.41929978797124	76.83806750266228
2	5.216683042871179	8.769260861061005
3	1.6057299477685267	4.0488557581553035
4	0.6800434400372343	2.2863103905418036
5	0.3413145782696385	1.4343772412144395
6	0.21720018617158815	1.0953426205637538
7	0.14609298236541346	0.8595396953035012
8	0.09179293582251642	0.6172168734922739
9	0.051714330040854316	0.39119379305848345
>10	0.21590732792056677	2.9252602525373264
>50	0.014221440761234938	0.734575011409819
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCACTG	40	0.0052293213	14.25	5
GAATATG	100	2.6921288E-4	9.500001	13
GGAATAT	105	4.3902814E-4	9.04762	12
TGGAATA	100	0.002851374	8.55	11
ATATGGC	125	0.0024703164	7.6	15
AATATGG	125	0.0024703164	7.6	14
>>END_MODULE