##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920829_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92026
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.862115054441134	33.0	32.0	34.0	32.0	34.0
2	32.96760698063591	33.0	32.0	34.0	32.0	34.0
3	33.183024362680115	33.0	33.0	34.0	32.0	34.0
4	33.31384608697542	34.0	33.0	34.0	32.0	35.0
5	33.13194097320323	34.0	33.0	34.0	32.0	35.0
6	36.024862538847714	37.0	37.0	37.0	33.0	37.0
7	35.988655380001305	37.0	37.0	37.0	33.0	37.0
8	35.972746832416924	37.0	37.0	37.0	33.0	37.0
9	35.95158976810901	37.0	37.0	37.0	33.0	37.0
10	35.939104166213895	37.0	37.0	37.0	33.0	37.0
11	35.86751570208419	37.0	35.0	37.0	33.0	37.0
12	35.82624475691652	37.0	35.0	37.0	33.0	37.0
13	37.48543889770282	38.0	38.0	38.0	37.0	38.0
14	37.496750918218765	38.0	38.0	38.0	37.0	38.0
15	37.49156760046074	38.0	38.0	38.0	37.0	38.0
16	37.47219264121009	38.0	38.0	38.0	37.0	38.0
17	37.401299632712494	38.0	38.0	38.0	37.0	38.0
18	37.44564579575337	38.0	38.0	38.0	37.0	38.0
19	37.43620281224871	38.0	38.0	38.0	37.0	38.0
20	37.43297546345598	38.0	38.0	38.0	37.0	38.0
21	37.43362745311108	38.0	38.0	38.0	37.0	38.0
22	37.45425205920066	38.0	38.0	38.0	37.0	38.0
23	38.12895268728403	39.0	38.0	39.0	37.0	39.0
24	38.11082737487232	39.0	38.0	39.0	37.0	39.0
25	37.99017668919653	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	4.0
24	21.0
25	31.0
26	83.0
27	147.0
28	269.0
29	416.0
30	654.0
31	957.0
32	1345.0
33	1989.0
34	3192.0
35	8833.0
36	56184.0
37	17898.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.30869536870015	18.615391302458	12.11070784343555	26.965205485406297
2	16.658335687740422	20.9799404516115	37.73281463934106	24.628909221307023
3	19.023971486319084	24.210549192619478	29.945884858626908	26.81959446243453
4	13.404907308804034	17.62436702670984	36.758090104970336	32.21263555951579
5	13.270162780083888	35.95505617977528	34.406580748918785	16.368200291222045
6	31.34440266881099	35.49214352465608	19.202181992045723	13.961271814487212
7	27.077130376198035	31.146632473431424	23.399908721448288	18.37632842892226
8	24.966857192532547	35.74207289244344	21.42655336535327	17.864516549670746
9	25.634059939582293	16.96803077391172	20.52354769304327	36.874361593462716
10	15.545606676374069	28.140960163432073	34.10014561102297	22.213287549170886
11	34.171864473083694	21.45154630213201	25.665572772912004	18.711016451872297
12	23.447721296155436	23.918240497250775	31.3237563297329	21.310281876860888
13	27.67804750831287	21.903592462999587	26.40449438202247	24.01386564666507
14	22.239367135374785	21.248342859626625	26.672896790037598	29.839393214960992
15	23.448807945580597	29.14067763458153	23.33036315823789	24.080151261599983
16	23.53899984786908	27.286853715254384	26.0448134222937	23.129333014582834
17	23.17497229043966	26.804381370482254	26.73592245669702	23.284723882381066
18	22.983721991611066	27.05322408884446	27.826918479560124	22.136135439984354
19	24.20837589376915	26.407754330297962	26.2328037728468	23.151066003086086
20	23.89324756047204	27.239041140547236	26.483819790059332	22.38389150892139
21	23.568339382348466	26.49685958316128	26.200204290091932	23.734596744398324
22	23.190185382391935	27.0086714624128	26.61965096820464	23.18149218699063
23	23.010888227240127	26.612044422228497	26.757655445200268	23.619411905331102
24	23.399908721448288	26.5859648360246	27.072783778497385	22.941342664029733
25	23.9725729685089	25.98287440505944	26.940212548627564	23.104340077804096
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	5.0
3	9.0
4	9.0
5	9.0
6	12.0
7	15.0
8	15.0
9	15.0
10	70.5
11	126.0
12	126.0
13	126.0
14	193.5
15	261.0
16	261.0
17	261.0
18	514.5
19	768.0
20	768.0
21	768.0
22	1312.5
23	1857.0
24	1857.0
25	1857.0
26	2735.5
27	3614.0
28	3614.0
29	3614.0
30	4755.0
31	5896.0
32	5896.0
33	5896.0
34	6888.5
35	7881.0
36	7881.0
37	7881.0
38	8936.5
39	9992.0
40	9992.0
41	9992.0
42	10714.5
43	11437.0
44	11437.0
45	11437.0
46	11725.0
47	12013.0
48	12013.0
49	12013.0
50	11733.5
51	11454.0
52	11454.0
53	11454.0
54	10622.5
55	9791.0
56	9791.0
57	9791.0
58	8597.0
59	7403.0
60	7403.0
61	7403.0
62	6162.5
63	4922.0
64	4922.0
65	4922.0
66	3871.0
67	2820.0
68	2820.0
69	2820.0
70	2005.0
71	1190.0
72	1190.0
73	1190.0
74	815.0
75	440.0
76	440.0
77	440.0
78	274.0
79	108.0
80	108.0
81	108.0
82	65.5
83	23.0
84	23.0
85	23.0
86	13.5
87	4.0
88	4.0
89	4.0
90	2.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	92026.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.83717645013365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.31325176275405	76.5544520026949
2	5.2701161343840734	8.836633125421077
3	1.6085130651182085	4.045595809879816
4	0.7154707590211531	2.3993219307586986
5	0.35384695147241807	1.4832764653467498
6	0.2125673994193281	1.0692630343598548
7	0.12831812525922853	0.7530480516375807
8	0.0777685607631688	0.5215917240779779
9	0.06999170468685192	0.5281116206289527
>10	0.2410825383658233	3.3338404363984093
>50	0.009072998755703028	0.47486579879599244
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGCG	55	0.0030308927	12.090909	16
AATATGG	110	6.9831486E-4	8.636364	14
ATATGGC	105	0.004430468	8.142858	15
>>END_MODULE