FastQCFastQC Report
Mon 15 Aug 2016
SRR2920828_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920828_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60374
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATATCAATGAGTTACAATGAA1000.16563421340312054No Hit
GATATACACTGTTCTACAAATCCCG910.1507271341968397No Hit
CTGAAGGACCTGGAATATGGCGAGA900.1490707920628085No Hit
GTCCTTCAGTGTGCATTTCTCATTT860.14244542352668368No Hit
CTGTAGGACGTGGAATATGGCAAGA760.12588200218637163No Hit
TTGTAGAACAGTGTATATCAATGAG720.1192566336502468No Hit
CTTTAGGACGTGAAATATGGCGAGG690.11428760724815318No Hit
GTCCTACAGTGGACATTTCTAAATT680.11263126511412197No Hit
CTGTAGGACCTGGAATATGGCGAGA620.10269321230993475No Hit
GTCCTAAAGTGTGTATTTCTCATTT610.10103687017590354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG307.6058414E-419.019
AAATCCC307.6058414E-419.018
CAAATCC307.6058414E-419.017
ACAAATC350.002137872416.28571516
GATATAC350.002137872416.2857151
CACTGTT350.002137872416.2857157
ACTGTTC400.005201719214.2500018
CTACAAA400.005201719214.25000114
TATACAC400.005201719214.2500013
TACAAAT400.005201719214.25000115
TACACTG700.001455755210.8571435
ACACTGT750.002585088410.1333346
TGTTCTA800.0044102379.50000110
TTCTACA1000.00282214168.54999912
Produced by FastQC (version 0.11.5)