FastQCFastQC Report
Mon 15 Aug 2016
SRR2920828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60374
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACACTGTTCTACAAATCCCG840.13913273925862127No Hit
GTGTATATCAATGAGTTACAATGAA790.13085102858846523No Hit
GTCCTTCAGTGTGCATTTCTCATTT750.12422566005234043No Hit
TTGTAGAACAGTGTATATCAATGAG730.12091297578427801No Hit
GTCCTACAGTGGACATTTCTAAATT730.12091297578427801No Hit
GTCCTAAAGTGTGTATTTCTCATTT710.1176002915162156No Hit
CTGAAGGACCTGGAATATGGCGAGA700.11594394938218439No Hit
ATTTAGAAATGTCCACTGTAGGACG670.11097492298009076No Hit
GTTTCTCATTGTAACTCATTGATAT660.10931858084605955No Hit
GATATACACTGTTCTACAATGCCGG650.10766223871202835No Hit
CTGTAGGACGTGGAATATGGCAAGA620.10269321230993475No Hit
TTTCTAAATTTTCCACCTTTTTCAG610.10103687017590354No Hit
CTGTAGGACCTGGAATATGGCGAGA610.10103687017590354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGAG250.00596661719.07
GTTTCCA550.00301011312.09090917
TGGAATA1150.0099139817.434782511
GGAATAT1150.0099139817.434782512
ATATGGC1450.00128976667.206896315
AATATGG1604.6307355E-47.125000514
Produced by FastQC (version 0.11.5)