##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920828_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	60374
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96485241991586	33.0	32.0	34.0	32.0	34.0
2	33.03744989565045	33.0	32.0	34.0	32.0	34.0
3	33.2432835326465	34.0	33.0	34.0	32.0	35.0
4	33.37295855831981	34.0	33.0	34.0	32.0	35.0
5	33.20700963991122	34.0	33.0	34.0	32.0	35.0
6	36.13842051214099	37.0	37.0	37.0	34.0	37.0
7	36.140159671381724	37.0	37.0	37.0	34.0	37.0
8	36.11831251863385	37.0	37.0	37.0	34.0	37.0
9	36.105840262364595	37.0	37.0	37.0	34.0	37.0
10	36.09187729817471	37.0	37.0	37.0	34.0	37.0
11	36.03108954185576	37.0	37.0	37.0	33.0	37.0
12	36.003544572166824	37.0	37.0	37.0	33.0	37.0
13	37.52348693146056	38.0	38.0	38.0	37.0	38.0
14	37.55561996886077	38.0	38.0	38.0	37.0	38.0
15	37.54506906946699	38.0	38.0	38.0	37.0	38.0
16	37.5160665187001	38.0	38.0	38.0	37.0	38.0
17	37.45580879186405	38.0	38.0	38.0	37.0	38.0
18	37.47558551694438	38.0	38.0	38.0	37.0	38.0
19	37.480107330970284	38.0	38.0	38.0	37.0	38.0
20	37.494948156491205	38.0	38.0	38.0	37.0	38.0
21	37.46192069433862	38.0	38.0	38.0	37.0	38.0
22	37.46977175605393	38.0	38.0	38.0	37.0	38.0
23	38.213220922913834	39.0	38.0	39.0	37.0	39.0
24	38.19962235399344	39.0	38.0	39.0	37.0	39.0
25	38.1169377546626	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	2.0
24	6.0
25	14.0
26	42.0
27	96.0
28	161.0
29	259.0
30	353.0
31	520.0
32	805.0
33	1110.0
34	1927.0
35	5197.0
36	35927.0
37	13953.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.22801205817074	19.77341239606453	12.58820021863716	26.41037532712757
2	15.518269453738364	22.39705833636996	39.724053400470396	22.360618809421272
3	18.24626494848776	25.90187829197999	31.19057872594163	24.66127803359062
4	12.51532116473979	19.447112995660383	38.904164044124954	29.13340179547487
5	11.841189916189087	36.376585947593334	35.28836916553483	16.493854970682744
6	28.29363633352105	36.89667737767913	21.204491999867493	13.60519428893232
7	24.460860635372843	31.854771921688148	24.932918143571737	18.751449299367277
8	22.652135025010768	37.63374962732302	22.961871004074602	16.752244343591613
9	24.40454500281578	18.092225130022857	22.3672441779574	35.13598568920396
10	14.690098386722761	29.275847219001555	35.596448802464636	20.437605591811046
11	32.184384006360354	22.405340047040117	27.53503163613476	17.87524431046477
12	22.584224997515488	24.790472720045052	33.00096067843774	19.62434160400172
13	26.635637857355814	23.2749196674065	27.586378242289726	22.50306423294796
14	20.936164574154436	23.35773677410806	28.0468413555504	27.6592572961871
15	21.717958061417168	30.966972537847422	24.384668897207405	22.93040050352801
16	21.567230927220326	28.876668764700035	28.268791201510584	21.28730910656905
17	21.600357769900953	28.026965249942027	28.47914665253255	21.893530327624475
18	21.249213237486337	28.66631331367807	29.903600887799385	20.18087256103621
19	23.269950641004407	27.448901845165135	27.816609798920066	21.464537714910392
20	21.89021764335641	28.555338390697983	28.828634842813134	20.725809123132475
21	22.1602014112035	28.065061119024747	27.34123960645311	22.433497863318646
22	21.562261900818232	28.5669327856362	28.820353132142973	21.05045218140259
23	21.3270613177858	28.126345777983904	28.42614370424355	22.12044919998675
24	21.623546559777388	28.023652565673967	29.0605227415775	21.292278132971145
25	22.566005234041146	27.265047868287674	28.701096498492728	21.467850399178452
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	9.0
7	18.0
8	18.0
9	18.0
10	56.0
11	94.0
12	94.0
13	94.0
14	174.0
15	254.0
16	254.0
17	254.0
18	432.0
19	610.0
20	610.0
21	610.0
22	1141.0
23	1672.0
24	1672.0
25	1672.0
26	2536.5
27	3401.0
28	3401.0
29	3401.0
30	4419.5
31	5438.0
32	5438.0
33	5438.0
34	6181.5
35	6925.0
36	6925.0
37	6925.0
38	7351.5
39	7778.0
40	7778.0
41	7778.0
42	7974.5
43	8171.0
44	8171.0
45	8171.0
46	7900.5
47	7630.0
48	7630.0
49	7630.0
50	7119.0
51	6608.0
52	6608.0
53	6608.0
54	5682.5
55	4757.0
56	4757.0
57	4757.0
58	4059.5
59	3362.0
60	3362.0
61	3362.0
62	2702.5
63	2043.0
64	2043.0
65	2043.0
66	1541.5
67	1040.0
68	1040.0
69	1040.0
70	717.5
71	395.0
72	395.0
73	395.0
74	265.5
75	136.0
76	136.0
77	136.0
78	83.5
79	31.0
80	31.0
81	31.0
82	21.0
83	11.0
84	11.0
85	11.0
86	5.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	60374.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.12107860999768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.73236558930552	79.78600059628317
2	3.634877702321418	6.188094212740584
3	1.1305481504543597	2.887004339616391
4	0.5098168940086786	1.7358465564647034
5	0.26074604502733945	1.1097492298009075
6	0.17318207468233737	0.8844866995726638
7	0.10507676441400245	0.6260973266637957
8	0.0817263723220019	0.5565309570344851
9	0.05253838220700122	0.40249113856958296
>10	0.29382576715767356	4.321396627687415
>50	0.025296258099667256	1.5023023155663036
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATATACACTGTTCTACAAATCCCG	84	0.13913273925862127	No Hit
GTGTATATCAATGAGTTACAATGAA	79	0.13085102858846523	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	75	0.12422566005234043	No Hit
TTGTAGAACAGTGTATATCAATGAG	73	0.12091297578427801	No Hit
GTCCTACAGTGGACATTTCTAAATT	73	0.12091297578427801	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	71	0.1176002915162156	No Hit
CTGAAGGACCTGGAATATGGCGAGA	70	0.11594394938218439	No Hit
ATTTAGAAATGTCCACTGTAGGACG	67	0.11097492298009076	No Hit
GTTTCTCATTGTAACTCATTGATAT	66	0.10931858084605955	No Hit
GATATACACTGTTCTACAATGCCGG	65	0.10766223871202835	No Hit
CTGTAGGACGTGGAATATGGCAAGA	62	0.10269321230993475	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	61	0.10103687017590354	No Hit
CTGTAGGACCTGGAATATGGCGAGA	61	0.10103687017590354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTGAG	25	0.005966617	19.0	7
GTTTCCA	55	0.003010113	12.090909	17
TGGAATA	115	0.009913981	7.4347825	11
GGAATAT	115	0.009913981	7.4347825	12
ATATGGC	145	0.0012897666	7.2068963	15
AATATGG	160	4.6307355E-4	7.1250005	14
>>END_MODULE