##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920827_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	91114
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88940228724455	33.0	32.0	33.0	30.0	34.0
2	32.20243870316307	33.0	32.0	34.0	32.0	34.0
3	32.32271659679083	33.0	32.0	34.0	32.0	34.0
4	32.473758149131854	33.0	32.0	34.0	32.0	34.0
5	32.48076036613473	33.0	32.0	34.0	32.0	34.0
6	35.570428254713875	37.0	37.0	37.0	33.0	37.0
7	35.55231907280989	37.0	37.0	37.0	33.0	37.0
8	35.41913427135237	37.0	35.0	37.0	33.0	37.0
9	35.56185657527932	37.0	35.0	37.0	33.0	37.0
10	35.54622780253309	37.0	35.0	37.0	33.0	37.0
11	35.535153763417256	37.0	35.0	37.0	33.0	37.0
12	35.53160875386878	37.0	35.0	37.0	33.0	37.0
13	37.038819500845094	38.0	38.0	38.0	37.0	38.0
14	37.051342274513246	38.0	38.0	38.0	37.0	38.0
15	36.99383190289088	38.0	38.0	38.0	37.0	38.0
16	37.03436354457054	38.0	38.0	38.0	37.0	38.0
17	37.0554140966262	38.0	38.0	38.0	37.0	38.0
18	37.04750093289725	38.0	38.0	38.0	37.0	38.0
19	37.02060056632351	38.0	38.0	38.0	37.0	38.0
20	37.028074719582065	38.0	38.0	38.0	37.0	38.0
21	37.07645367342011	38.0	38.0	38.0	37.0	38.0
22	37.08139254121211	38.0	38.0	38.0	37.0	38.0
23	37.693954825822594	39.0	38.0	39.0	37.0	39.0
24	37.62357047215576	39.0	38.0	39.0	37.0	39.0
25	37.55923348771868	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	3.0
19	12.0
20	31.0
21	79.0
22	127.0
23	198.0
24	315.0
25	402.0
26	508.0
27	593.0
28	709.0
29	912.0
30	1048.0
31	1305.0
32	1776.0
33	2309.0
34	4012.0
35	10773.0
36	55657.0
37	10342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44917356278947	19.40206773931558	12.868494413591764	26.28026428430318
2	15.591456856246023	21.243716662642402	39.47143139363874	23.693395087472837
3	18.03125754549246	25.025791865135982	30.779024079724298	26.163926509647258
4	12.309853590008121	18.68538314638804	38.88315736330312	30.121605900300725
5	12.589722764887942	36.04166209364094	35.32168492218539	16.04693021928573
6	28.984568781965447	36.94053603178436	20.36020809096297	13.714687095287223
7	25.355049717935774	31.572535504971793	24.630682441776237	18.441732335316196
8	23.742783765392804	35.90337379546502	22.67269574379349	17.681146695348684
9	25.312246197071804	17.13677371205303	21.65858155717014	35.89239853370503
10	14.574050091094673	28.535680575981736	35.3140022389534	21.57626709397019
11	32.38909497991527	22.477336084465616	26.7159821761749	18.417586759444212
12	22.33026757688171	24.81616436552012	32.39238755844327	20.461180499154903
13	26.791711482318853	22.756107733169436	26.93548741137476	23.51669337313695
14	21.61029040542617	21.831990692978028	27.820093509230194	28.737625392365608
15	22.459775665649627	29.8461268301249	24.456175779792346	23.23792172443313
16	22.744034945233444	28.082402265294025	26.954145356366748	22.21941743310578
17	22.13929802225783	27.40522861470246	27.738876572206244	22.716596790833464
18	21.96918146497794	28.04398884913405	28.65860350769366	21.328226178194353
19	23.62425093838488	27.1835283271506	27.31413394209452	21.878086792369995
20	22.918541607217332	27.8135083521742	27.88923765831815	21.378712382290317
21	23.00524617512128	27.26913536887855	26.985973615470726	22.739644840529447
22	22.350023048049696	27.918870865070133	27.8135083521742	21.917597734705975
23	22.481726189169613	27.15718769892662	27.780582566894218	22.58050354500955
24	22.25124569220976	27.527054020238385	28.133985995565997	22.087714291985865
25	22.92183418574533	26.84219768641482	27.604978378734334	22.630989749105517
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	2.5
3	4.0
4	4.0
5	4.0
6	16.0
7	28.0
8	28.0
9	28.0
10	78.5
11	129.0
12	129.0
13	129.0
14	278.5
15	428.0
16	428.0
17	428.0
18	746.0
19	1064.0
20	1064.0
21	1064.0
22	1706.5
23	2349.0
24	2349.0
25	2349.0
26	3374.0
27	4399.0
28	4399.0
29	4399.0
30	5694.5
31	6990.0
32	6990.0
33	6990.0
34	7993.5
35	8997.0
36	8997.0
37	8997.0
38	10001.0
39	11005.0
40	11005.0
41	11005.0
42	11438.0
43	11871.0
44	11871.0
45	11871.0
46	11838.5
47	11806.0
48	11806.0
49	11806.0
50	11231.5
51	10657.0
52	10657.0
53	10657.0
54	9533.5
55	8410.0
56	8410.0
57	8410.0
58	7174.5
59	5939.0
60	5939.0
61	5939.0
62	4890.5
63	3842.0
64	3842.0
65	3842.0
66	2892.5
67	1943.0
68	1943.0
69	1943.0
70	1394.5
71	846.0
72	846.0
73	846.0
74	574.0
75	302.0
76	302.0
77	302.0
78	194.0
79	86.0
80	86.0
81	86.0
82	51.0
83	16.0
84	16.0
85	16.0
86	9.0
87	2.0
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	91114.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.1280593542156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.24137888090463	83.05309831639485
2	3.545587008231939	6.24931404614
3	1.000037361296188	2.6439405579823076
4	0.44833555425482896	1.5804376934389888
5	0.20424175249386653	0.899971464319424
6	0.1295191601180617	0.6848563338235617
7	0.09464861700935277	0.5838839256316264
8	0.08219485161338531	0.5794938209276291
9	0.05853269736104712	0.4642535724477028
>10	0.18680648093951205	2.784423908510218
>50	0.00871763577717723	0.47632636038369514
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCTG	40	0.0052288002	14.25	6
ACACTGT	60	0.005804031	11.083334	6
TACACTG	60	0.005804031	11.083334	5
GACCTGG	60	0.005804031	11.083334	7
>>END_MODULE