##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920827_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	91114
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0331123647299	33.0	33.0	34.0	32.0	34.0
2	33.09827249379898	33.0	33.0	34.0	32.0	34.0
3	33.3422306122001	34.0	33.0	34.0	32.0	35.0
4	33.417564808920694	34.0	33.0	34.0	32.0	35.0
5	33.298845402462845	34.0	33.0	34.0	32.0	35.0
6	36.14740874069847	37.0	37.0	37.0	34.0	37.0
7	36.119443773734005	37.0	37.0	37.0	34.0	37.0
8	36.06888074280572	37.0	37.0	37.0	34.0	37.0
9	36.04812652281757	37.0	37.0	37.0	34.0	37.0
10	36.036690300063654	37.0	37.0	37.0	33.0	37.0
11	35.98980398182497	37.0	37.0	37.0	33.0	37.0
12	35.9620585200957	37.0	37.0	37.0	33.0	37.0
13	37.51883354918015	38.0	38.0	38.0	37.0	38.0
14	37.5343745198323	38.0	38.0	38.0	37.0	38.0
15	37.54596439625085	38.0	38.0	38.0	37.0	38.0
16	37.51552999539039	38.0	38.0	38.0	37.0	38.0
17	37.44801018504291	38.0	38.0	38.0	37.0	38.0
18	37.49321728823232	38.0	38.0	38.0	37.0	38.0
19	37.49436969071712	38.0	38.0	38.0	37.0	38.0
20	37.49903417696512	38.0	38.0	38.0	37.0	38.0
21	37.45697697390083	38.0	38.0	38.0	37.0	38.0
22	37.48155058498145	38.0	38.0	38.0	37.0	38.0
23	38.19278047281428	39.0	38.0	39.0	37.0	39.0
24	38.1887964527954	39.0	38.0	39.0	37.0	39.0
25	38.068518559167636	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	0.0
21	0.0
22	3.0
23	3.0
24	16.0
25	34.0
26	65.0
27	135.0
28	202.0
29	418.0
30	572.0
31	837.0
32	1189.0
33	1744.0
34	2876.0
35	7675.0
36	53118.0
37	22226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.421735408389495	19.706082490067388	13.007880237943676	25.864301863599447
2	15.966810808437781	21.506025418706237	39.18936716640692	23.337796606449064
3	18.25076278069232	25.348464560879773	30.523300480716465	25.877472177711436
4	12.56228461048796	18.775380292819985	38.794257743047176	29.868077353644885
5	12.551309348727969	36.21946133415282	35.21083477840946	16.01839453870975
6	29.30833900388524	36.77919968391246	20.343745198322978	13.568716113879317
7	25.554799481967645	31.448515047083873	24.60982944443225	18.38685602651623
8	23.67144456395285	36.42360120288869	22.17771143841781	17.727242794740654
9	24.86226046491209	17.618587703316724	21.791382224466055	35.727769607305135
10	14.580635248150669	28.991153939021448	34.88377197796167	21.544438834866213
11	32.43189850077924	22.572820861777554	26.64135039620695	18.353930241236256
12	22.51574950062559	24.647145334416226	32.51860306868319	20.318502096274997
13	27.085848497486666	22.707816581425465	27.085848497486666	23.120486423601204
14	21.19762056325043	22.112957394033845	28.079109686766028	28.61031235594969
15	22.4992866079856	29.647474592269024	24.253133437232478	23.600105362512895
16	22.459775665649627	28.68713918826964	26.92451214961477	21.928572996465967
17	22.229295168689774	27.55449217463837	28.01984327326207	22.196369383409795
18	22.01527756436991	27.960576859758106	28.667383717101654	21.35676185877033
19	23.394867967601026	27.00353403428672	27.414008824110454	22.1875891740018
20	22.76927804728143	28.07801216059003	27.73009636279825	21.42261342933029
21	22.757205259345437	27.431569242926447	27.23401453124657	22.57721096648155
22	22.40599688302566	27.6554645828303	27.692780472814277	22.245758061329763
23	22.153565862545822	27.160480277454617	27.98252738327809	22.703426476721468
24	22.247953113681763	27.575345171982352	28.079109686766028	22.097592027569856
25	22.884518295761357	26.890488838158788	27.632416533134315	22.59257633294554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	2.0
3	4.0
4	4.0
5	4.0
6	21.0
7	38.0
8	38.0
9	38.0
10	101.0
11	164.0
12	164.0
13	164.0
14	288.5
15	413.0
16	413.0
17	413.0
18	725.5
19	1038.0
20	1038.0
21	1038.0
22	1715.5
23	2393.0
24	2393.0
25	2393.0
26	3405.5
27	4418.0
28	4418.0
29	4418.0
30	5722.5
31	7027.0
32	7027.0
33	7027.0
34	8053.0
35	9079.0
36	9079.0
37	9079.0
38	9986.5
39	10894.0
40	10894.0
41	10894.0
42	11524.0
43	12154.0
44	12154.0
45	12154.0
46	11964.0
47	11774.0
48	11774.0
49	11774.0
50	11205.5
51	10637.0
52	10637.0
53	10637.0
54	9469.5
55	8302.0
56	8302.0
57	8302.0
58	7103.0
59	5904.0
60	5904.0
61	5904.0
62	4822.5
63	3741.0
64	3741.0
65	3741.0
66	2868.0
67	1995.0
68	1995.0
69	1995.0
70	1398.5
71	802.0
72	802.0
73	802.0
74	528.0
75	254.0
76	254.0
77	254.0
78	159.0
79	64.0
80	64.0
81	64.0
82	39.5
83	15.0
84	15.0
85	15.0
86	9.5
87	4.0
88	4.0
89	4.0
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	91114.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.83831244375179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.10368223107967	82.6590864192111
2	3.579773343495808	6.288824988475975
3	1.0445691152399634	2.752595649406238
4	0.46105981282720876	1.619948635774963
5	0.21990928741893967	0.9658230348793819
6	0.17242887308985044	0.9087516737274185
7	0.08996289030774805	0.5531531927036459
8	0.07122062149363388	0.5004719362556797
9	0.051228868091912086	0.40498715894374077
>10	0.19741856484200268	2.901859209342143
>50	0.008746392113253282	0.44449810127971556
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE