##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920826_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	213548
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14200086163298	33.0	32.0	34.0	32.0	34.0
2	32.403867046284674	33.0	32.0	34.0	32.0	34.0
3	32.64410343342012	33.0	32.0	34.0	32.0	34.0
4	32.75185906681402	33.0	32.0	34.0	32.0	34.0
5	32.86179219660217	33.0	33.0	34.0	32.0	34.0
6	35.62570007679772	37.0	35.0	37.0	33.0	37.0
7	35.64003877348418	37.0	37.0	37.0	33.0	37.0
8	35.49086388072003	37.0	35.0	37.0	33.0	37.0
9	35.600656526869834	37.0	35.0	37.0	33.0	37.0
10	35.590129619570305	37.0	35.0	37.0	33.0	37.0
11	35.5178367392811	37.0	34.0	37.0	33.0	37.0
12	35.5282137973664	37.0	34.0	37.0	33.0	37.0
13	37.196845674040496	38.0	38.0	38.0	37.0	38.0
14	37.18384157191826	38.0	38.0	38.0	37.0	38.0
15	37.13835296982411	38.0	38.0	38.0	37.0	38.0
16	37.16004364358364	38.0	38.0	38.0	37.0	38.0
17	37.16462341019349	38.0	38.0	38.0	37.0	38.0
18	37.18593946091745	38.0	38.0	38.0	37.0	38.0
19	37.15917264502594	38.0	38.0	38.0	37.0	38.0
20	37.18379006125087	38.0	38.0	38.0	37.0	38.0
21	37.207358532976194	38.0	38.0	38.0	37.0	38.0
22	37.2200442055182	38.0	38.0	38.0	37.0	38.0
23	37.85127465487853	39.0	38.0	39.0	37.0	39.0
24	37.75445333133534	39.0	38.0	39.0	37.0	39.0
25	37.67921029464102	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	2.0
18	3.0
19	20.0
20	56.0
21	133.0
22	221.0
23	351.0
24	549.0
25	708.0
26	872.0
27	1130.0
28	1470.0
29	1731.0
30	2190.0
31	2745.0
32	3632.0
33	4886.0
34	8543.0
35	24277.0
36	133162.0
37	26865.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99923202277708	17.914473560979264	12.273587202877104	26.81270721336655
2	17.041133609305636	19.87749826736846	37.92964579391987	25.151722329406034
3	19.246726731226705	23.318879127877572	28.99488639556446	28.439507745331262
4	13.44053795867908	16.75220559312192	36.36372150523536	33.443534942963645
5	14.581265101991123	35.15322082154832	34.673235057223664	15.592279019236893
6	32.75516511510293	34.92938355779497	18.22962518965291	14.085826137449192
7	28.247045160806938	29.973120797197822	22.83374229681383	18.946091745181413
8	25.630303257347293	34.33466948882687	21.165264952141907	18.86976230168393
9	26.23625601738251	15.70466592990803	19.74029258059078	38.318785472118684
10	15.431191113941598	27.475321707531798	33.60368629067001	23.489800887856592
11	35.15181598516493	21.33665499091539	24.47131324105119	19.04021578286849
12	24.08685635079701	23.297806582126736	30.53224567778673	22.083091389289528
13	28.557045722741492	21.1615187217862	25.473429861202163	24.80800569427014
14	23.150298761870864	19.91542884971997	26.13276640380617	30.801505984602993
15	24.250753928859087	27.85977859778598	22.81828909659655	25.071178376758386
16	24.663307546781052	26.295259145484856	24.74338322063424	24.298050087099856
17	23.587671155899375	26.201603386592243	26.356135388765058	23.854590068743327
18	23.73752037012756	26.166482477007513	26.475078202558677	23.620918950306255
19	24.76024125723491	25.792327720231516	25.776874520014236	23.67055650251934
20	24.782250360574672	25.848521175567086	25.863974375784366	23.505254088073876
21	24.371569857830558	25.5033997040478	25.708974094817087	24.416056343304547
22	24.38421338528106	25.656526869837226	25.671511791260045	24.28774795362167
23	24.24841253488677	25.5553786502332	25.69398917339427	24.502219641485755
24	24.127128327120833	25.79139116264259	26.135576076572946	23.945904433663625
25	24.418397737276866	25.538052334838067	25.761421319796955	24.28212860808811
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	2.5
3	5.0
4	5.0
5	5.0
6	18.5
7	32.0
8	32.0
9	32.0
10	83.5
11	135.0
12	135.0
13	135.0
14	269.5
15	404.0
16	404.0
17	404.0
18	864.0
19	1324.0
20	1324.0
21	1324.0
22	2187.5
23	3051.0
24	3051.0
25	3051.0
26	4666.5
27	6282.0
28	6282.0
29	6282.0
30	8488.5
31	10695.0
32	10695.0
33	10695.0
34	13110.5
35	15526.0
36	15526.0
37	15526.0
38	18257.0
39	20988.0
40	20988.0
41	20988.0
42	23353.5
43	25719.0
44	25719.0
45	25719.0
46	27521.0
47	29323.0
48	29323.0
49	29323.0
50	29114.5
51	28906.0
52	28906.0
53	28906.0
54	27194.5
55	25483.0
56	25483.0
57	25483.0
58	22760.5
59	20038.0
60	20038.0
61	20038.0
62	16722.0
63	13406.0
64	13406.0
65	13406.0
66	10393.0
67	7380.0
68	7380.0
69	7380.0
70	5286.0
71	3192.0
72	3192.0
73	3192.0
74	2193.0
75	1194.0
76	1194.0
77	1194.0
78	765.5
79	337.0
80	337.0
81	337.0
82	214.5
83	92.0
84	92.0
85	92.0
86	61.0
87	30.0
88	30.0
89	30.0
90	17.5
91	5.0
92	5.0
93	5.0
94	3.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	213548.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.27617471528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.50909536050506	79.74097953430208
2	3.6217905642119907	6.177048898717886
3	1.159386065349155	2.9660402601352676
4	0.5439374710740189	1.8553962726998239
5	0.3283239933665116	1.399910711077054
6	0.21079996837582632	1.078572895991548
7	0.13200845956381715	0.7880023523161334
8	0.09926285485778588	0.6771805242872023
9	0.0696027419056843	0.5341910021470508
>10	0.3159383057404945	4.242889252697128
>50	0.00985421504966637	0.5397882956288009
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	4.6827879446307153E-4
9	0.0	0.0	0.0	0.0	4.6827879446307153E-4
10	0.0	0.0	0.0	0.0	4.6827879446307153E-4
11	0.0	0.0	0.0	0.0	4.6827879446307153E-4
12	0.0	0.0	0.0	0.0	4.6827879446307153E-4
13	0.0	0.0	0.0	0.0	4.6827879446307153E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCCCC	40	2.7471472E-4	16.625	14
TCCCCCC	40	2.7471472E-4	16.625	15
CTGTAGA	50	0.001492172	13.299999	1
CCCCCCT	60	0.0058475733	11.083334	16
CATCCCC	60	0.0058475733	11.083334	13
GTCTGGA	75	0.0026316112	10.133333	1
TCCAGCA	80	0.0044885776	9.5	2
TTGTTCT	80	0.0044885776	9.5	4
AATATGG	160	0.0037500216	6.53125	14
>>END_MODULE