##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920826_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	213548
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.08057673216326	33.0	33.0	34.0	32.0	34.0
2	33.1614765766947	33.0	33.0	34.0	32.0	35.0
3	33.38721036956562	34.0	33.0	34.0	32.0	35.0
4	33.45929720718527	34.0	33.0	34.0	32.0	35.0
5	33.357671343210896	34.0	33.0	34.0	32.0	35.0
6	36.05414707700376	37.0	37.0	37.0	33.0	37.0
7	36.027164852866804	37.0	37.0	37.0	34.0	37.0
8	35.987042725757206	37.0	37.0	37.0	33.0	37.0
9	35.959217599790215	37.0	37.0	37.0	33.0	37.0
10	35.94869069249068	37.0	37.0	37.0	33.0	37.0
11	35.86866652930489	37.0	35.0	37.0	33.0	37.0
12	35.83023957143125	37.0	35.0	37.0	33.0	37.0
13	37.52354037499766	38.0	38.0	38.0	37.0	38.0
14	37.54213104313784	38.0	38.0	38.0	37.0	38.0
15	37.524313035008525	38.0	38.0	38.0	37.0	38.0
16	37.511290201734504	38.0	38.0	38.0	37.0	38.0
17	37.448208365332384	38.0	38.0	38.0	37.0	38.0
18	37.4757103789312	38.0	38.0	38.0	37.0	38.0
19	37.48599846404555	38.0	38.0	38.0	37.0	38.0
20	37.484579579298334	38.0	38.0	38.0	37.0	38.0
21	37.46427969355836	38.0	38.0	38.0	37.0	38.0
22	37.47968606589619	38.0	38.0	38.0	37.0	38.0
23	38.16964804165808	39.0	38.0	39.0	37.0	39.0
24	38.16095678723285	39.0	38.0	39.0	37.0	39.0
25	38.0302601756982	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0
22	5.0
23	9.0
24	17.0
25	98.0
26	165.0
27	351.0
28	589.0
29	968.0
30	1457.0
31	2019.0
32	2848.0
33	4035.0
34	6960.0
35	18411.0
36	125365.0
37	50249.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.47547155674603	18.011407271433118	12.22863243860865	26.284488733212207
2	17.25654185475865	20.011426002584898	37.85331635042239	24.878715792234065
3	19.22846385824264	23.576900743626723	28.841290950980575	28.353344447150054
4	13.665780058815816	17.047221233633657	36.40727143312043	32.8797272744301
5	14.677262254856052	35.5198831176129	34.18247888062637	15.620375746904678
6	32.619364264708636	34.96028995822953	18.248356341431435	14.171989435630397
7	28.544402195290992	29.974057354786748	22.683893082585648	18.797647367336616
8	25.574578080806187	34.841347144435915	20.896004645325643	18.68807012943226
9	25.915485043175305	16.220709161406337	19.86719613389027	37.99660966152808
10	15.475209320621126	27.703841759229775	33.361586153932606	23.459362766216493
11	35.031936613782385	21.451851574353306	24.49519545956881	19.021016352295504
12	24.069530035401876	23.384906437896866	30.455447955494787	22.09011557120647
13	28.679266488096356	21.13529510929627	25.499185194897635	24.68625320770974
14	22.895086818888494	20.106486597860904	26.075636390881673	30.922790192368925
15	24.30179631745556	28.122951280274222	22.68717103414689	24.888081368123327
16	24.767733717946317	26.30321988499073	24.85811152527769	24.070934871785266
17	23.665405435780247	26.039110644913556	26.339277352164387	23.956206567141813
18	23.619514113922865	26.394065971116564	26.48538033603686	23.501039578923706
19	24.805196021503363	25.87193511529024	25.84805289677262	23.474815966433777
20	24.666585498342293	26.089216475921106	25.885046921535203	23.3591511042014
21	24.370165021447168	25.575982917189577	25.62046940266357	24.433382658699685
22	24.331297881506732	25.814805102365746	25.64809785153689	24.20579916459063
23	24.239515237791974	25.587221608256694	25.86491093337329	24.308352220578044
24	24.076554217318822	26.03161818420215	25.848521175567086	24.043306422911943
25	24.546706126959748	25.404592878416093	25.718339670706353	24.330361323917806
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	3.0
3	5.0
4	5.0
5	5.0
6	15.0
7	25.0
8	25.0
9	25.0
10	95.5
11	166.0
12	166.0
13	166.0
14	290.5
15	415.0
16	415.0
17	415.0
18	861.0
19	1307.0
20	1307.0
21	1307.0
22	2248.5
23	3190.0
24	3190.0
25	3190.0
26	4779.0
27	6368.0
28	6368.0
29	6368.0
30	8489.0
31	10610.0
32	10610.0
33	10610.0
34	13137.0
35	15664.0
36	15664.0
37	15664.0
38	18457.0
39	21250.0
40	21250.0
41	21250.0
42	23669.5
43	26089.0
44	26089.0
45	26089.0
46	27525.5
47	28962.0
48	28962.0
49	28962.0
50	28953.0
51	28944.0
52	28944.0
53	28944.0
54	27249.5
55	25555.0
56	25555.0
57	25555.0
58	22538.5
59	19522.0
60	19522.0
61	19522.0
62	16503.0
63	13484.0
64	13484.0
65	13484.0
66	10378.0
67	7272.0
68	7272.0
69	7272.0
70	5182.0
71	3092.0
72	3092.0
73	3092.0
74	2146.5
75	1201.0
76	1201.0
77	1201.0
78	760.0
79	319.0
80	319.0
81	319.0
82	202.0
83	85.0
84	85.0
85	85.0
86	52.5
87	20.0
88	20.0
89	20.0
90	10.5
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	213548.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.83978625268023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.31577932262563	79.16890771733836
2	3.7169936130804415	6.306978872726446
3	1.179821330517192	3.0028736849229407
4	0.5868149489119461	1.9914101934226789
5	0.338552514379992	1.4361361477652985
6	0.21386159626853038	1.0886383269047468
7	0.12933123686468226	0.7680704143976078
8	0.09230344376424361	0.6264803551475774
9	0.06829971242845903	0.5215079703194992
>10	0.3489076255661236	4.574228696655693
>50	0.008751239618183105	0.4642807960907852
>100	5.834159745455403E-4	0.05048682430837963
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.6827879446307153E-4	0.0
4	0.0	0.0	0.0	4.6827879446307153E-4	0.0
5	0.0	0.0	0.0	4.6827879446307153E-4	0.0
6	0.0	0.0	0.0	4.6827879446307153E-4	0.0
7	0.0	0.0	0.0	4.6827879446307153E-4	0.0
8	0.0	0.0	0.0	4.6827879446307153E-4	0.0
9	0.0	0.0	0.0	4.6827879446307153E-4	0.0
10	0.0	0.0	0.0	4.6827879446307153E-4	0.0
11	0.0	0.0	0.0	4.6827879446307153E-4	0.0
12	0.0	0.0	0.0	4.6827879446307153E-4	0.0
13	0.0	0.0	0.0	4.6827879446307153E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACC	25	0.0060154246	19.0	4
GTGCTAG	25	0.0060154246	19.0	1
TCCTAGA	40	2.7471472E-4	16.625	2
GTACATG	40	0.005259393	14.25	1
AACACCA	50	0.001492172	13.299999	5
TCCAAGA	65	5.409649E-5	13.153846	2
GTCCAAG	60	4.0663948E-4	12.666667	1
CTGGGCA	85	0.0073910896	8.941176	9
AGCACTG	85	0.0073910896	8.941176	5
ATATGGC	105	0.00447959	8.142858	15
AATATGG	105	0.00447959	8.142858	14
>>END_MODULE