##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920825_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	144024
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09120007776482	33.0	32.0	34.0	32.0	34.0
2	32.27446814419819	33.0	32.0	34.0	32.0	34.0
3	32.37146586679998	33.0	32.0	34.0	32.0	34.0
4	32.50175665166917	33.0	32.0	34.0	32.0	34.0
5	32.428414708659666	33.0	32.0	34.0	32.0	34.0
6	35.37422235183025	37.0	34.0	37.0	33.0	37.0
7	35.234884463700496	37.0	34.0	37.0	33.0	37.0
8	35.2672957284897	37.0	34.0	37.0	33.0	37.0
9	35.34024884741432	37.0	34.0	37.0	33.0	37.0
10	35.350372160195526	37.0	34.0	37.0	33.0	37.0
11	35.314773926567796	37.0	34.0	37.0	33.0	37.0
12	35.346081208687444	37.0	34.0	37.0	33.0	37.0
13	37.091193134477585	38.0	38.0	38.0	37.0	38.0
14	37.084104038215855	38.0	38.0	38.0	37.0	38.0
15	37.02260734322057	38.0	38.0	38.0	36.0	38.0
16	37.044756429483975	38.0	38.0	38.0	37.0	38.0
17	37.06291312559018	38.0	38.0	38.0	37.0	38.0
18	37.07687607620952	38.0	38.0	38.0	37.0	38.0
19	37.044666166749984	38.0	38.0	38.0	37.0	38.0
20	37.067509581736374	38.0	38.0	38.0	37.0	38.0
21	37.111488363050604	38.0	38.0	38.0	37.0	38.0
22	37.123055879575624	38.0	38.0	38.0	37.0	38.0
23	37.70945120257735	39.0	38.0	39.0	37.0	39.0
24	37.626124812531245	39.0	38.0	39.0	36.0	39.0
25	37.515289118480254	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	4.0
17	1.0
18	4.0
19	13.0
20	74.0
21	116.0
22	199.0
23	338.0
24	447.0
25	553.0
26	800.0
27	885.0
28	1081.0
29	1379.0
30	1668.0
31	2127.0
32	2704.0
33	3949.0
34	6734.0
35	19100.0
36	88464.0
37	13383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42956729433983	17.64289285119147	12.327112147975336	26.600427706493363
2	17.900488807420984	19.091262567349887	36.37935344109315	26.628895184135974
3	19.466894406487807	22.680942065211354	28.756318391379214	29.09584513692162
4	14.095567405432426	16.109120702105205	34.884463700494365	34.91084819196801
5	15.141226462256292	34.917097150474916	32.76328945175804	17.178386935510748
6	34.26720546575571	33.688829639504526	17.311698050324946	14.73226684441482
7	28.94378714658668	29.97833694384269	21.82900072210187	19.248875187468755
8	27.07395989557296	33.43123368327501	20.023051713603287	19.47175470754874
9	26.329639504527023	15.866799977781481	19.265539076820527	38.53802144087097
10	16.588901849691716	26.759428984058214	32.51610842637338	24.135560739876688
11	35.821113147808695	21.003443870466036	23.979336777203798	19.196106204521467
12	24.575070821529746	22.913542187413206	29.096539465644618	23.41484752541243
13	28.712575681830803	20.520885407987556	25.378409154029885	25.388129756151752
14	23.996000666555574	19.372465700161083	24.766011220352162	31.86552241293118
15	24.86391157029384	27.293367772037996	21.970643781591956	25.87207687607621
16	25.23329445092485	25.367299894462036	24.381353107815364	25.018052546797755
17	24.925012497917017	25.06526689996112	25.088179747819805	24.92154085430206
18	24.711159251235905	25.686691107037717	25.08193078931289	24.520218852413485
19	25.478392490140532	25.025690162750653	25.215936232850076	24.279981114258735
20	25.460339943342774	25.210381603066157	24.690329389546186	24.638949064044883
21	24.881269788368606	25.02291284785869	24.969449536188414	25.12636782758429
22	24.618813531078153	24.897239348997388	25.14997500416597	25.333972115758485
23	24.929178470254957	24.2084652557907	25.354801977448204	25.507554296506136
24	24.709770593789923	25.47700383269455	25.117341554185412	24.695884019330112
25	25.0812364605899	24.53341109815031	25.087485419096815	25.297867022162972
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	3.0
4	3.0
5	3.0
6	11.5
7	20.0
8	20.0
9	20.0
10	43.0
11	66.0
12	66.0
13	66.0
14	155.0
15	244.0
16	244.0
17	244.0
18	508.5
19	773.0
20	773.0
21	773.0
22	1185.0
23	1597.0
24	1597.0
25	1597.0
26	2387.5
27	3178.0
28	3178.0
29	3178.0
30	4297.5
31	5417.0
32	5417.0
33	5417.0
34	6776.0
35	8135.0
36	8135.0
37	8135.0
38	10300.0
39	12465.0
40	12465.0
41	12465.0
42	14376.5
43	16288.0
44	16288.0
45	16288.0
46	17954.0
47	19620.0
48	19620.0
49	19620.0
50	20156.5
51	20693.0
52	20693.0
53	20693.0
54	20139.5
55	19586.0
56	19586.0
57	19586.0
58	17615.0
59	15644.0
60	15644.0
61	15644.0
62	13197.5
63	10751.0
64	10751.0
65	10751.0
66	8302.5
67	5854.0
68	5854.0
69	5854.0
70	4165.0
71	2476.0
72	2476.0
73	2476.0
74	1682.0
75	888.0
76	888.0
77	888.0
78	570.5
79	253.0
80	253.0
81	253.0
82	158.0
83	63.0
84	63.0
85	63.0
86	36.5
87	10.0
88	10.0
89	10.0
90	5.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	144024.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.09281786368938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.25889670643419	56.6933288896295
2	8.633629040481289	11.757762595123035
3	3.131436728867136	6.396850524912515
4	1.621290914652799	4.415930678220296
5	0.9401447945345162	3.200855412986724
6	0.5822371775262567	2.378770204965839
7	0.42928520444580404	2.0461867466533357
8	0.28754970939125113	1.566405599066822
9	0.22636892015907004	1.3872687885352442
>10	0.8718262465585805	9.26998278064767
>50	0.015295197308045275	0.7005776814975282
>100	0.0020393596410727033	0.1860800977614842
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAAAC	25	0.0060061193	19.0	3
TGGACAG	65	7.9461397E-4	11.692307	5
ATGGGGG	60	0.005831905	11.083333	5
CATGGGG	70	0.0014772192	10.857142	4
GTACATG	80	0.0044735954	9.5	1
>>END_MODULE