##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920825_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	144024
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03368188635228	33.0	33.0	34.0	32.0	34.0
2	33.14401072043548	33.0	33.0	34.0	32.0	34.0
3	33.282286285619065	34.0	33.0	34.0	32.0	35.0
4	33.362022996167305	34.0	33.0	34.0	32.0	35.0
5	33.241286174526465	34.0	33.0	34.0	32.0	35.0
6	35.91545853468866	37.0	37.0	37.0	33.0	37.0
7	35.941252846747766	37.0	37.0	37.0	33.0	37.0
8	35.88617174915292	37.0	37.0	37.0	33.0	37.0
9	35.870125812364606	37.0	35.0	37.0	33.0	37.0
10	35.85847497639282	37.0	35.0	37.0	33.0	37.0
11	35.7670179970005	37.0	35.0	37.0	33.0	37.0
12	35.73955729600622	37.0	35.0	37.0	33.0	37.0
13	37.49323723823807	38.0	38.0	38.0	37.0	38.0
14	37.52488474143198	38.0	38.0	38.0	37.0	38.0
15	37.52285730156085	38.0	38.0	38.0	37.0	38.0
16	37.49791701383103	38.0	38.0	38.0	37.0	38.0
17	37.41753457757041	38.0	38.0	38.0	37.0	38.0
18	37.45552824529245	38.0	38.0	38.0	37.0	38.0
19	37.45272315725157	38.0	38.0	38.0	37.0	38.0
20	37.46152724545909	38.0	38.0	38.0	37.0	38.0
21	37.456569738376935	38.0	38.0	38.0	37.0	38.0
22	37.462020218852416	38.0	38.0	38.0	37.0	38.0
23	38.14628117535966	39.0	38.0	39.0	37.0	39.0
24	38.13729656168417	39.0	38.0	39.0	37.0	39.0
25	38.010727378770206	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	5.0
24	23.0
25	47.0
26	105.0
27	215.0
28	402.0
29	678.0
30	1018.0
31	1412.0
32	1993.0
33	2879.0
34	4897.0
35	14072.0
36	88444.0
37	27833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6246736655002	17.513747708715215	12.48819641170916	26.37338221407543
2	18.253902127423206	19.51341443092818	36.20715991779148	26.025523523857135
3	19.73143364994723	22.766344498139198	28.489001833027828	29.013220018885743
4	14.215686274509803	16.293117813697716	34.86224518135867	34.628950730433814
5	15.08498583569405	34.89071265900128	32.75218019219019	17.27212131311448
6	34.15472421263123	33.756179525634614	17.368633005610175	14.720463256123981
7	29.13681053157807	29.822112981169806	21.878992390157194	19.16208409709493
8	26.91356996056213	33.842970616008444	19.898072543464977	19.345386879964448
9	26.385880131089262	16.1611953563295	19.132227962006333	38.32069655057491
10	16.42504027106593	26.95731267011054	32.405015830694886	24.212631228128643
11	35.83430539354552	21.10481586402266	23.714103205021388	19.346775537410434
12	24.629228461923013	22.84549797256013	29.313170027217684	23.21210353829917
13	28.725073598844634	20.473671054824198	25.512414597567073	25.288840748764095
14	24.036271732489027	19.56201744153752	24.870854857523746	31.5308559684497
15	24.936816086207855	27.473198911292563	21.969949452868967	25.620035549630614
16	25.345081375326334	25.356190634894183	24.441759706715548	24.856968283063935
17	24.984030439371217	25.151363661611953	24.984030439371217	24.880575459645616
18	24.80628228628562	25.854024329278452	25.05762928400822	24.282064100427707
19	25.452702327389883	25.165944564794756	25.116647225462422	24.264705882352942
20	25.50894295395212	25.42215186357829	24.595206354496472	24.473698827973113
21	25.1180358829084	25.134005443537188	24.895156362828416	24.85280231072599
22	24.89585069155141	24.98819641170916	25.09720602121869	25.018746875520748
23	24.95209131811365	24.43412209076265	25.108315280786535	25.505471310337164
24	24.61117591512526	25.72626784424818	24.894462034105427	24.768094206521134
25	25.052074654224292	24.767399877798145	25.09442870632672	25.086096761650833
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	3.0
4	3.0
5	3.0
6	9.0
7	15.0
8	15.0
9	15.0
10	60.5
11	106.0
12	106.0
13	106.0
14	188.0
15	270.0
16	270.0
17	270.0
18	532.0
19	794.0
20	794.0
21	794.0
22	1227.0
23	1660.0
24	1660.0
25	1660.0
26	2493.0
27	3326.0
28	3326.0
29	3326.0
30	4393.5
31	5461.0
32	5461.0
33	5461.0
34	6778.5
35	8096.0
36	8096.0
37	8096.0
38	10263.0
39	12430.0
40	12430.0
41	12430.0
42	14379.0
43	16328.0
44	16328.0
45	16328.0
46	17976.0
47	19624.0
48	19624.0
49	19624.0
50	20034.5
51	20445.0
52	20445.0
53	20445.0
54	20073.5
55	19702.0
56	19702.0
57	19702.0
58	17686.0
59	15670.0
60	15670.0
61	15670.0
62	13174.5
63	10679.0
64	10679.0
65	10679.0
66	8234.0
67	5789.0
68	5789.0
69	5789.0
70	4136.5
71	2484.0
72	2484.0
73	2484.0
74	1662.0
75	840.0
76	840.0
77	840.0
78	539.5
79	239.0
80	239.0
81	239.0
82	144.0
83	49.0
84	49.0
85	49.0
86	29.5
87	10.0
88	10.0
89	10.0
90	7.0
91	4.0
92	4.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	144024.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.34710881519746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.60340632603406	55.63100594345387
2	8.947791661511815	12.052157973671054
3	3.2753927997030807	6.61764705882353
4	1.7330611571611203	4.668666333388879
5	0.9155016701719658	3.08281953007832
6	0.6474493793558497	2.6162306282286285
7	0.4371314281001278	2.0607676498361385
8	0.32372468967792484	1.7441537521524193
9	0.1948533960163306	1.1810531578070322
>10	0.9041609963297454	9.467172137977004
>50	0.01443358489009856	0.6339221240904294
>100	0.0030929110478782633	0.24440371049269566
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGAG	25	0.0060061193	19.0	11
CAGGACA	40	0.0052483873	14.25	4
GCATGCC	40	0.0052483873	14.25	13
>>END_MODULE