##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920824_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	119421
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.811105249495483	33.0	32.0	33.0	30.0	34.0
2	32.13382905854079	33.0	32.0	34.0	31.0	34.0
3	32.280361075522734	33.0	32.0	34.0	32.0	34.0
4	32.39591864077507	33.0	32.0	34.0	32.0	34.0
5	32.36848627963256	33.0	32.0	34.0	32.0	34.0
6	35.54025673876454	37.0	35.0	37.0	33.0	37.0
7	35.56447358504786	37.0	35.0	37.0	33.0	37.0
8	35.53509014327464	37.0	35.0	37.0	33.0	37.0
9	35.58001524020064	37.0	35.0	37.0	33.0	37.0
10	35.54475343532545	37.0	35.0	37.0	33.0	37.0
11	35.48991383425026	37.0	34.0	37.0	33.0	37.0
12	35.48460488523794	37.0	34.0	37.0	33.0	37.0
13	37.18598906389998	38.0	38.0	38.0	37.0	38.0
14	37.181333266343444	38.0	38.0	38.0	37.0	38.0
15	37.10944473752522	38.0	38.0	38.0	37.0	38.0
16	37.15897538958809	38.0	38.0	38.0	37.0	38.0
17	37.16007234908433	38.0	38.0	38.0	37.0	38.0
18	37.15955317741436	38.0	38.0	38.0	37.0	38.0
19	37.13217105869152	38.0	38.0	38.0	37.0	38.0
20	37.15552541010375	38.0	38.0	38.0	37.0	38.0
21	37.161906197402466	38.0	38.0	38.0	37.0	38.0
22	37.20382512288459	38.0	38.0	38.0	37.0	38.0
23	37.808936451712846	39.0	38.0	39.0	37.0	39.0
24	37.73343046867804	39.0	38.0	39.0	37.0	39.0
25	37.62653134708301	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	10.0
19	13.0
20	44.0
21	75.0
22	181.0
23	222.0
24	313.0
25	421.0
26	535.0
27	677.0
28	831.0
29	1007.0
30	1256.0
31	1555.0
32	2025.0
33	2848.0
34	5243.0
35	15947.0
36	76359.0
37	9854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.313152628097235	17.62504082196599	12.464306947689268	26.59749960224751
2	17.7623701024108	19.334957838236157	36.57648152335016	26.32619053600288
3	19.466425503052225	23.337603939005703	28.809840815266995	28.386129742675077
4	13.876118940554843	16.30031568987029	35.33549375737935	34.48807161219551
5	14.748662295576153	34.833906934291285	33.32830909136584	17.089121678766716
6	33.566123211160516	34.22513628256337	17.551351939776087	14.657388566500028
7	28.262198440810245	29.822225571716864	22.622486832299177	19.29308915517371
8	26.65444101121243	33.72857370144279	20.17400624680751	19.44297904053726
9	25.84972492275228	16.338834878287738	19.2311235042413	38.58031669471869
10	16.24086215992162	26.77418544477102	32.92636973396639	24.05858266134097
11	35.652858374992675	21.360564724797147	23.93297661215364	19.05360028805654
12	24.50155332814162	23.135796886644727	29.221828656601435	23.14082112861222
13	28.48828932934743	20.728347610554255	25.52063707388148	25.26272598621683
14	23.642407951700285	19.453864898133492	25.127908826755764	31.775818323410455
15	24.488992723222882	27.35197327103273	22.405607054035723	25.753426951708665
16	24.861624002478628	25.666340090938778	24.730993711323805	24.741042195258792
17	24.77956138367624	25.348975473325464	25.25602699692684	24.615436146071463
18	24.608737156781473	25.86396027499351	25.348975473325464	24.178327094899558
19	25.055057318227114	25.392518903710403	25.44611081803033	24.106312960032156
20	25.109486606208286	25.39921789300039	24.99811590926219	24.493179591529127
21	24.479781612949147	25.154704783915726	25.51728757923648	24.848226023898647
22	24.60789978312022	25.16894013615696	25.227556292444376	24.995603788278444
23	24.90181793821857	24.467221008030414	25.269424975506823	25.361536078244196
24	24.248666482444463	25.953559256747138	25.217507808509392	24.58026645229901
25	24.929451269039784	24.972994699424724	25.446948191691575	24.650605839843916
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	3.0
4	3.0
5	3.0
6	20.5
7	38.0
8	38.0
9	38.0
10	65.0
11	92.0
12	92.0
13	92.0
14	208.0
15	324.0
16	324.0
17	324.0
18	566.5
19	809.0
20	809.0
21	809.0
22	1193.0
23	1577.0
24	1577.0
25	1577.0
26	2217.5
27	2858.0
28	2858.0
29	2858.0
30	3810.5
31	4763.0
32	4763.0
33	4763.0
34	5979.0
35	7195.0
36	7195.0
37	7195.0
38	8926.5
39	10658.0
40	10658.0
41	10658.0
42	12189.0
43	13720.0
44	13720.0
45	13720.0
46	14984.0
47	16248.0
48	16248.0
49	16248.0
50	16500.0
51	16752.0
52	16752.0
53	16752.0
54	16341.5
55	15931.0
56	15931.0
57	15931.0
58	14079.5
59	12228.0
60	12228.0
61	12228.0
62	10377.5
63	8527.0
64	8527.0
65	8527.0
66	6602.5
67	4678.0
68	4678.0
69	4678.0
70	3373.5
71	2069.0
72	2069.0
73	2069.0
74	1392.0
75	715.0
76	715.0
77	715.0
78	456.0
79	197.0
80	197.0
81	197.0
82	114.5
83	32.0
84	32.0
85	32.0
86	19.0
87	6.0
88	6.0
89	6.0
90	3.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	119421.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.43718441480141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.64441865644524	60.46758945244136
2	8.267398108098604	11.811992865576407
3	2.8941168196363893	6.202426708870299
4	1.3937241387394357	3.9825491328995737
5	0.7935670663806543	2.834509843327388
6	0.533342710787589	2.286030095209385
7	0.33993271676571607	1.6998685323351839
8	0.23795290173600123	1.3598948258681471
9	0.18520472154821768	1.1907453462958775
>10	0.698620342042644	7.608377086107134
>50	0.011721817819507449	0.5560161110692424
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATACC	25	0.0060002366	19.0	4
TGCCGGT	25	0.0060002366	19.0	19
CATACCA	30	7.6658186E-4	18.999998	5
CACATAC	35	0.0021544613	16.285715	3
CCAGAAC	40	0.005241432	14.25	3
TCCAGAA	120	0.0016623887	7.916666	2
>>END_MODULE