##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920824_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	119421
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07218160959965	33.0	33.0	34.0	32.0	34.0
2	33.18617328610546	33.0	33.0	34.0	32.0	34.0
3	33.318729536681154	34.0	33.0	34.0	32.0	35.0
4	33.40837038711784	34.0	33.0	34.0	32.0	35.0
5	33.28093886334899	34.0	33.0	34.0	32.0	35.0
6	35.92960199629881	37.0	37.0	37.0	33.0	37.0
7	35.965734669781696	37.0	37.0	37.0	33.0	37.0
8	35.904790614716006	37.0	37.0	37.0	33.0	37.0
9	35.883831151974945	37.0	35.0	37.0	33.0	37.0
10	35.90098056455732	37.0	35.0	37.0	33.0	37.0
11	35.79705411945972	37.0	35.0	37.0	33.0	37.0
12	35.77715812126846	37.0	35.0	37.0	33.0	37.0
13	37.5220606091056	38.0	38.0	38.0	37.0	38.0
14	37.540340476130666	38.0	38.0	38.0	37.0	38.0
15	37.54198172850671	38.0	38.0	38.0	37.0	38.0
16	37.50787549928405	38.0	38.0	38.0	37.0	38.0
17	37.45238274675308	38.0	38.0	38.0	37.0	38.0
18	37.48003282504752	38.0	38.0	38.0	37.0	38.0
19	37.4891350767453	38.0	38.0	38.0	37.0	38.0
20	37.4821011379908	38.0	38.0	38.0	37.0	38.0
21	37.4662747757932	38.0	38.0	38.0	37.0	38.0
22	37.49247619765368	38.0	38.0	38.0	37.0	38.0
23	38.16551527788245	39.0	38.0	39.0	37.0	39.0
24	38.17157786318989	39.0	38.0	39.0	37.0	39.0
25	38.03604056238015	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	1.0
22	4.0
23	6.0
24	25.0
25	50.0
26	91.0
27	158.0
28	324.0
29	509.0
30	755.0
31	1116.0
32	1613.0
33	2323.0
34	3900.0
35	11341.0
36	72676.0
37	24528.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49151321794324	17.804238785473242	12.307718073035732	26.396529923547785
2	18.130814513360296	19.752807295199336	36.30684720442803	25.809530987012337
3	19.547650748193366	23.24381808894583	28.592123663342296	28.61640749951851
4	13.995863374113432	16.585022734694903	34.714162500732705	34.704951390458966
5	14.763735021478636	35.16801902512958	32.94646670183636	17.12177925155542
6	33.616365630835446	33.99820802036493	17.502784267423653	14.882642081375971
7	28.822401420185727	30.052503328560302	21.941702045703853	19.18339320555011
8	26.594987481263765	34.26030597633582	19.89683556493414	19.247870977466274
9	26.139456209544388	16.464440927475067	19.534252769613385	37.86185009336716
10	16.173034893360462	27.01618643287194	32.73042429723416	24.080354376533442
11	35.60261595531774	21.420018254745816	23.63738370973279	19.33998208020365
12	24.429539193274213	23.12742315003224	29.22852764589143	23.21451001080212
13	28.242101472940266	21.136985957243702	25.56418050426642	25.056732065549607
14	23.542760486011673	19.5962184205458	25.1371199370295	31.723901156413024
15	24.72680684301756	27.4491086157376	22.388022207149497	25.436062334095343
16	25.0290987347284	25.709046147662473	24.581941199621507	24.679913917987623
17	24.716758359082576	25.318830021520505	25.13795731069075	24.826454308706175
18	24.567705847380278	25.829627954882305	25.569204746233915	24.033461451503506
19	25.246815886653103	25.287009822393046	25.412615871580375	24.053558419373477
20	25.33474012108423	25.621959286892594	24.81556845110994	24.22773214091324
21	24.63971998224768	25.360698704582944	25.08017852806458	24.919402785104797
22	24.49234221786788	25.311293658569262	25.15972902588322	25.03663509767964
23	24.712571490776327	24.67405230235888	25.390006782726655	25.22336942413813
24	24.434563435241706	25.808693613351085	25.178988620091943	24.577754331315262
25	25.08185327538708	24.93866237931352	25.282822954086804	24.6966613912126
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	20.5
7	40.0
8	40.0
9	40.0
10	90.5
11	141.0
12	141.0
13	141.0
14	217.0
15	293.0
16	293.0
17	293.0
18	564.0
19	835.0
20	835.0
21	835.0
22	1252.5
23	1670.0
24	1670.0
25	1670.0
26	2270.0
27	2870.0
28	2870.0
29	2870.0
30	3809.5
31	4749.0
32	4749.0
33	4749.0
34	5883.5
35	7018.0
36	7018.0
37	7018.0
38	8832.0
39	10646.0
40	10646.0
41	10646.0
42	12208.0
43	13770.0
44	13770.0
45	13770.0
46	14920.5
47	16071.0
48	16071.0
49	16071.0
50	16304.5
51	16538.0
52	16538.0
53	16538.0
54	16257.5
55	15977.0
56	15977.0
57	15977.0
58	14292.5
59	12608.0
60	12608.0
61	12608.0
62	10538.5
63	8469.0
64	8469.0
65	8469.0
66	6598.0
67	4727.0
68	4727.0
69	4727.0
70	3373.5
71	2020.0
72	2020.0
73	2020.0
74	1366.0
75	712.0
76	712.0
77	712.0
78	464.5
79	217.0
80	217.0
81	217.0
82	127.0
83	37.0
84	37.0
85	37.0
86	23.5
87	10.0
88	10.0
89	10.0
90	5.5
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	119421.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.93141072340711
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.3543036584934	59.83369759087598
2	8.388917090677275	11.900754473668785
3	2.9218364479913115	6.217499434772779
4	1.4579668740481897	4.136625886569364
5	0.8275585252694583	2.934994682677251
6	0.5371456904388067	2.286030095209385
7	0.3541619936959165	1.7584846886226042
8	0.2361079957972777	1.3397978579981744
9	0.17471991688998548	1.1153817167834803
>10	0.7366569468875064	7.956724529186659
>50	0.010624859810877496	0.5200090436355415
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACAAG	40	2.7344318E-4	16.625	1
AGCCCAG	40	0.005241432	14.25	13
ATCCCCC	50	0.0014854575	13.299999	14
CCCCCTG	50	0.0014854575	13.299999	17
CCCCCCT	50	0.0014854575	13.299999	16
TCCCCCC	50	0.0014854575	13.299999	15
CATCCCC	50	0.0014854575	13.299999	13
CTGGGCC	60	4.04309E-4	12.666666	9
TACACAG	60	4.04309E-4	12.666666	5
>>END_MODULE