##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920823_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117075
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19357676702968	33.0	32.0	34.0	32.0	34.0
2	32.300858424087124	33.0	32.0	34.0	32.0	34.0
3	32.575263719837714	33.0	32.0	34.0	32.0	34.0
4	32.696322869955154	33.0	32.0	34.0	32.0	34.0
5	32.70330130258382	33.0	32.0	34.0	32.0	34.0
6	35.568379244074315	37.0	35.0	37.0	33.0	37.0
7	35.460815716421095	37.0	35.0	37.0	33.0	37.0
8	35.435660901131754	37.0	35.0	37.0	33.0	37.0
9	35.51882980995089	37.0	34.0	37.0	33.0	37.0
10	35.52784112748238	37.0	34.0	37.0	33.0	37.0
11	35.48225496476618	37.0	34.0	37.0	33.0	37.0
12	35.50160153747598	37.0	34.0	37.0	33.0	37.0
13	37.20330557335042	38.0	38.0	38.0	37.0	38.0
14	37.197335041639974	38.0	38.0	38.0	37.0	38.0
15	37.14175528507367	38.0	38.0	38.0	37.0	38.0
16	37.16348494554772	38.0	38.0	38.0	37.0	38.0
17	37.169831304719196	38.0	38.0	38.0	37.0	38.0
18	37.16742259235533	38.0	38.0	38.0	37.0	38.0
19	37.138039718129406	38.0	38.0	38.0	37.0	38.0
20	37.175357676702966	38.0	38.0	38.0	37.0	38.0
21	37.18764040145206	38.0	38.0	38.0	37.0	38.0
22	37.217168481742476	38.0	38.0	38.0	37.0	38.0
23	37.83271407217595	39.0	38.0	39.0	37.0	39.0
24	37.754755498612	39.0	38.0	39.0	37.0	39.0
25	37.64116164851591	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	11.0
20	48.0
21	73.0
22	115.0
23	212.0
24	310.0
25	407.0
26	486.0
27	621.0
28	814.0
29	981.0
30	1164.0
31	1487.0
32	1925.0
33	2747.0
34	4919.0
35	14326.0
36	73577.0
37	12848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28165705744181	17.812513346145632	12.061499039077514	26.844330557335045
2	17.887678838351484	19.344864403160365	36.294682895579754	26.472773862908394
3	19.459320948110186	22.86824685030963	28.791800128122997	28.880632073457186
4	13.920990817851806	16.01537475976938	35.25346999786462	34.8101644245142
5	15.010890454836643	34.89216314328422	33.24706384796071	16.849882553918427
6	33.97309417040359	33.96882340380098	17.53149690369421	14.526585522101218
7	28.538116591928254	29.883408071748878	22.20456972026479	19.37390561605808
8	26.682895579756565	33.65364082852872	20.151185137732224	19.51227845398249
9	26.238735853085625	15.92568866111467	19.39611360239163	38.43946188340807
10	16.286995515695068	26.6598334401025	32.71919709587871	24.333973948323724
11	35.738629083920564	21.234251548152894	23.771086910100365	19.25603245782618
12	24.218663250053385	23.154388212684175	29.515267990604315	23.111680546658125
13	28.69357249626308	20.33995302156737	25.524663677130043	25.441810805039506
14	23.731795857356396	19.349989323083495	25.130044843049326	31.788169976510783
15	24.947256032457826	27.458466794789665	22.0021353833013	25.592141789451205
16	25.36237454623105	25.4537689515268	24.569720264787527	24.614136237454623
17	24.559470424941278	25.039504591074095	25.52978859705317	24.871236386931454
18	24.486013239376465	25.765534913516973	25.44608157164211	24.302370275464448
19	25.53833013025838	25.070254110612854	25.136878069613495	24.254537689515267
20	25.310271193679267	25.346999786461673	24.65171898355755	24.691010036301517
21	24.767029681827886	25.439248345077942	25.048900277599827	24.74482169549434
22	24.748238308776425	25.15481528934444	24.98910954516336	25.10783685671578
23	24.769592141789452	24.8447576339953	24.99338031176596	25.392269912449283
24	24.63634422378817	25.616058082425795	24.906256673072814	24.841341020713216
25	25.048900277599827	24.64659406363442	25.32820841341021	24.976297245355543
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	3.0
3	4.0
4	4.0
5	4.0
6	14.0
7	24.0
8	24.0
9	24.0
10	63.0
11	102.0
12	102.0
13	102.0
14	195.5
15	289.0
16	289.0
17	289.0
18	515.5
19	742.0
20	742.0
21	742.0
22	1106.5
23	1471.0
24	1471.0
25	1471.0
26	2113.5
27	2756.0
28	2756.0
29	2756.0
30	3678.0
31	4600.0
32	4600.0
33	4600.0
34	5761.5
35	6923.0
36	6923.0
37	6923.0
38	8480.5
39	10038.0
40	10038.0
41	10038.0
42	11595.5
43	13153.0
44	13153.0
45	13153.0
46	14610.5
47	16068.0
48	16068.0
49	16068.0
50	16213.5
51	16359.0
52	16359.0
53	16359.0
54	15895.5
55	15432.0
56	15432.0
57	15432.0
58	14002.5
59	12573.0
60	12573.0
61	12573.0
62	10621.0
63	8669.0
64	8669.0
65	8669.0
66	6740.5
67	4812.0
68	4812.0
69	4812.0
70	3484.5
71	2157.0
72	2157.0
73	2157.0
74	1388.5
75	620.0
76	620.0
77	620.0
78	419.0
79	218.0
80	218.0
81	218.0
82	134.0
83	50.0
84	50.0
85	50.0
86	29.5
87	9.0
88	9.0
89	9.0
90	6.5
91	4.0
92	4.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	117075.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.2716207559257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.60856616082825	61.14798206278027
2	8.379426086134355	12.111894084988254
3	2.932208197418806	6.357463164638053
4	1.40405616224649	4.058936579115952
5	0.7823949321609227	2.827247490924621
6	0.5129296080934147	2.2242152466367715
7	0.360468964213114	1.8236173393124067
8	0.21155391670212265	1.22314755498612
9	0.17255235663972013	1.122357463164638
>10	0.6299342882806221	6.789664744821695
>50	0.005909327282182197	0.3134742686312193
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGTCC	35	0.0021541198	16.285715	8
ACACAGT	70	0.0014736365	10.857143	6
>>END_MODULE