FastQCFastQC Report
Mon 15 Aug 2016
SRR2920823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117075
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG402.7338532E-416.6253
AGAGGTG508.6047745E-515.25
TAGAGGT456.6964875E-414.7777784
CCCTAGA551.9358993E-413.8181831
TGGAGCC752.0427666E-411.40000110
CAGAAGA600.005820840611.0833344
CCTAGAG600.005820840611.0833342
GTGACAC600.005820840611.08333419
GAGGTGG803.7148639E-410.68756
GCCCAGT750.00261643310.13333414
AGGTGGA856.4964726E-410.0588247
AGCCCAG950.0017973889.00000113
CCAGAAG850.00734955178.9411763
Produced by FastQC (version 0.11.5)