##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920822_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116183
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10690892815644	33.0	32.0	34.0	32.0	34.0
2	32.28837265348631	33.0	32.0	34.0	32.0	34.0
3	32.45006584440064	33.0	32.0	34.0	32.0	34.0
4	32.65079228458553	33.0	32.0	34.0	32.0	34.0
5	32.667025296299805	33.0	32.0	34.0	32.0	34.0
6	35.41366637115585	37.0	34.0	37.0	33.0	37.0
7	35.464215935205665	37.0	35.0	37.0	33.0	37.0
8	35.44289612077498	37.0	34.0	37.0	33.0	37.0
9	35.49523596395342	37.0	34.0	37.0	33.0	37.0
10	35.48121497981632	37.0	34.0	37.0	33.0	37.0
11	35.41881342365062	37.0	34.0	37.0	33.0	37.0
12	35.42873742285877	37.0	34.0	37.0	33.0	37.0
13	37.14832634722808	38.0	38.0	38.0	37.0	38.0
14	37.13811831335049	38.0	38.0	38.0	37.0	38.0
15	37.084797259495794	38.0	38.0	38.0	37.0	38.0
16	37.1080881023902	38.0	38.0	38.0	37.0	38.0
17	37.09475568714872	38.0	38.0	38.0	37.0	38.0
18	37.114620899787404	38.0	38.0	38.0	37.0	38.0
19	37.094239260477	38.0	38.0	38.0	37.0	38.0
20	37.12514739677922	38.0	38.0	38.0	37.0	38.0
21	37.11704810514447	38.0	38.0	38.0	37.0	38.0
22	37.17616174483358	38.0	38.0	38.0	37.0	38.0
23	37.752149626021016	39.0	38.0	39.0	37.0	39.0
24	37.661396245578096	39.0	38.0	39.0	37.0	39.0
25	37.56405842507079	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	3.0
18	5.0
19	13.0
20	40.0
21	86.0
22	149.0
23	242.0
24	321.0
25	397.0
26	559.0
27	693.0
28	840.0
29	1056.0
30	1246.0
31	1586.0
32	2085.0
33	2867.0
34	5106.0
35	14649.0
36	71536.0
37	12702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03899882082577	17.693638484115574	12.37616518767806	26.8911975073806
2	17.81672017420793	19.2239828546345	36.70416498110739	26.255131990050177
3	19.545028102218055	22.579034798550563	28.74430854772213	29.13162855150926
4	14.03991978172366	15.747570642865135	35.08000309856003	35.13250647685117
5	15.384350550424761	34.548083626692375	33.276813303151066	16.790752519731804
6	34.10653882237505	33.67015828477488	17.468132170799514	14.75517072205056
7	29.12302144031399	29.875282958780545	21.64516323386382	19.356532367041652
8	27.043543375536867	33.485105394076584	19.767952282175532	19.703398948211014
9	26.283535456994567	15.67527090882487	19.255829166056994	38.78536446812356
10	16.437000249606225	26.49268825903962	32.56414449618275	24.506166995171412
11	35.74275066059578	21.202757718426966	23.819319521788902	19.23517209918835
12	24.66711997452295	22.607438265494952	29.0851501510548	23.640291608927296
13	28.404327655508983	20.360982243529605	25.270478469311346	25.96421163165007
14	24.049129390702596	19.260993432774157	24.79450522021294	31.895371956310303
15	24.93910468829347	26.985875730528562	22.12802217191844	25.94699740925953
16	25.663823450935162	25.361713847981203	24.058597213017393	24.915865488066242
17	25.128461134589397	25.011404422333733	24.792783797973886	25.06735064510298
18	24.531127617637694	25.717187540345833	25.087147000852106	24.664537841164368
19	25.40991367067471	25.180964512880543	25.0742363340592	24.334885482385545
20	25.643166384066518	24.995911622182245	24.478624239346548	24.88229775440469
21	24.957179621803537	24.942547532771574	24.885740598882798	25.214532246542092
22	24.973533133074547	24.655070018849575	24.942547532771574	25.4288493153043
23	25.13018255682845	24.37878174948142	24.96664744411833	25.524388249571793
24	24.787619531256723	25.243796424606007	24.911561932468608	25.05702211166866
25	25.38753518156701	24.530266906518168	24.768683886627134	25.31351402528769
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.0
4	1.0
5	1.0
6	10.5
7	20.0
8	20.0
9	20.0
10	49.0
11	78.0
12	78.0
13	78.0
14	157.0
15	236.0
16	236.0
17	236.0
18	461.0
19	686.0
20	686.0
21	686.0
22	1023.5
23	1361.0
24	1361.0
25	1361.0
26	1898.0
27	2435.0
28	2435.0
29	2435.0
30	3278.0
31	4121.0
32	4121.0
33	4121.0
34	5197.0
35	6273.0
36	6273.0
37	6273.0
38	8084.0
39	9895.0
40	9895.0
41	9895.0
42	11411.0
43	12927.0
44	12927.0
45	12927.0
46	14328.5
47	15730.0
48	15730.0
49	15730.0
50	16339.5
51	16949.0
52	16949.0
53	16949.0
54	16490.5
55	16032.0
56	16032.0
57	16032.0
58	14429.5
59	12827.0
60	12827.0
61	12827.0
62	10747.0
63	8667.0
64	8667.0
65	8667.0
66	6741.0
67	4815.0
68	4815.0
69	4815.0
70	3460.0
71	2105.0
72	2105.0
73	2105.0
74	1416.5
75	728.0
76	728.0
77	728.0
78	478.0
79	228.0
80	228.0
81	228.0
82	141.5
83	55.0
84	55.0
85	55.0
86	33.0
87	11.0
88	11.0
89	11.0
90	6.5
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	116183.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.87238236230773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.63765652728137	56.91452277871978
2	9.141693119048213	12.592203678679326
3	3.2917593541453174	6.801339266501984
4	1.679622085030868	4.6271829785769
5	0.9435377040165962	3.249184476214248
6	0.6436051888325127	2.659597359338285
7	0.36866704991376936	1.7773684618231582
8	0.28118673298507835	1.5492800151485158
9	0.2112024794421255	1.3091416128004958
>10	0.7923217276112875	8.102734479226738
>50	0.00749831287960209	0.3296523587788231
>100	0.0012497188132670149	0.08779253419174922
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGAGG	25	0.0059992727	19.0	3
ACAATGG	25	0.0059992727	19.0	3
TAGAGGT	35	0.0021539847	16.285715	4
ACATGGG	35	0.0021539847	16.285715	3
CCTAGAG	50	0.0014850318	13.299999	2
CCCTAGA	60	0.0058203833	11.083333	1
ACACCTT	60	0.0058203833	11.083333	6
>>END_MODULE