##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920822_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116183
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.072953874491105	33.0	33.0	34.0	32.0	34.0
2	33.17604124527685	33.0	33.0	34.0	32.0	34.0
3	33.315330125749895	34.0	33.0	34.0	32.0	35.0
4	33.40305380305208	34.0	33.0	34.0	32.0	35.0
5	33.291514249072584	34.0	33.0	34.0	32.0	35.0
6	35.91331778315244	37.0	37.0	37.0	33.0	37.0
7	35.95189485552964	37.0	37.0	37.0	33.0	37.0
8	35.8981090176704	37.0	37.0	37.0	33.0	37.0
9	35.88483685220729	37.0	35.0	37.0	33.0	37.0
10	35.89065525937529	37.0	35.0	37.0	33.0	37.0
11	35.7802002014064	37.0	35.0	37.0	33.0	37.0
12	35.76484511503404	37.0	35.0	37.0	33.0	37.0
13	37.50945491164801	38.0	38.0	38.0	37.0	38.0
14	37.52975908695765	38.0	38.0	38.0	38.0	38.0
15	37.52933733850907	38.0	38.0	38.0	37.0	38.0
16	37.5201965864197	38.0	38.0	38.0	37.0	38.0
17	37.44993673773271	38.0	38.0	38.0	37.0	38.0
18	37.480715767367	38.0	38.0	38.0	37.0	38.0
19	37.49146604924989	38.0	38.0	38.0	37.0	38.0
20	37.488711773667404	38.0	38.0	38.0	37.0	38.0
21	37.47100694593873	38.0	38.0	38.0	37.0	38.0
22	37.47456168286238	38.0	38.0	38.0	37.0	38.0
23	38.15098594458742	39.0	38.0	39.0	37.0	39.0
24	38.144367076078254	39.0	38.0	39.0	37.0	39.0
25	38.04556604666776	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	4.0
23	9.0
24	17.0
25	45.0
26	85.0
27	177.0
28	321.0
29	513.0
30	730.0
31	1106.0
32	1593.0
33	2309.0
34	3818.0
35	10662.0
36	71448.0
37	23345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73875696100118	17.88471635265056	12.183365896904021	26.193160789444235
2	18.434710758028285	19.381492989507933	36.19892755394507	25.984868698518714
3	19.841112727335325	22.78302333387845	28.526548634481806	28.84931530430442
4	14.514171608583013	15.748431353984662	34.8777359854712	34.85966105196113
5	15.403286195054353	35.051599631615645	32.79825792069408	16.74685625263593
6	34.123753044765586	33.784632863671966	17.473296437516677	14.618317654045773
7	29.193599752115198	29.93639344826696	21.685616656481585	19.18439014313626
8	26.934233063356945	33.80442921942108	19.886730416670254	19.374607300551716
9	26.20176790063951	15.992873311930317	19.09917974230309	38.70617904512708
10	16.40773607154231	26.923043818803094	32.42815213929749	24.241067970357108
11	35.97428195174853	21.4721602988389	23.440606629197042	19.11295112021552
12	24.51133126188857	22.849298090082026	29.25471024160161	23.38466040642779
13	28.898375838117452	20.22326846440529	25.356549581264044	25.521806116213213
14	23.747880498868167	19.30919325546767	25.06821135622251	31.87471488944166
15	25.044972155995282	27.158017954433955	22.028179682053313	25.76883020751745
16	25.661241317576582	25.362574559100732	23.846862277613763	25.129321845708922
17	24.682612774674435	25.19817873527108	25.013986555692313	25.10522193436217
18	24.626666551905185	25.71546611810678	25.126739712350343	24.531127617637694
19	25.397863715001336	25.291135536179993	24.821187264918276	24.4898134839004
20	25.448645671053427	25.308349758570532	24.692080596989232	24.550923973386812
21	25.0647685117444	25.15256104593615	24.94082611053252	24.841844331786923
22	24.901233399034282	24.883158465524215	24.85303357634077	25.362574559100732
23	25.16116815713142	24.27119285954055	24.996772333301774	25.570866650026254
24	24.668841396762005	25.589802294655843	24.998493755540828	24.742862553041324
25	25.51750256061558	24.482927794944185	24.848730020743137	25.150839623697095
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	10.5
7	20.0
8	20.0
9	20.0
10	75.0
11	130.0
12	130.0
13	130.0
14	185.0
15	240.0
16	240.0
17	240.0
18	507.0
19	774.0
20	774.0
21	774.0
22	1111.5
23	1449.0
24	1449.0
25	1449.0
26	1985.5
27	2522.0
28	2522.0
29	2522.0
30	3365.5
31	4209.0
32	4209.0
33	4209.0
34	5223.0
35	6237.0
36	6237.0
37	6237.0
38	8046.0
39	9855.0
40	9855.0
41	9855.0
42	11353.5
43	12852.0
44	12852.0
45	12852.0
46	14315.5
47	15779.0
48	15779.0
49	15779.0
50	16249.0
51	16719.0
52	16719.0
53	16719.0
54	16371.0
55	16023.0
56	16023.0
57	16023.0
58	14434.0
59	12845.0
60	12845.0
61	12845.0
62	10797.0
63	8749.0
64	8749.0
65	8749.0
66	6739.0
67	4729.0
68	4729.0
69	4729.0
70	3402.5
71	2076.0
72	2076.0
73	2076.0
74	1402.5
75	729.0
76	729.0
77	729.0
78	464.5
79	200.0
80	200.0
81	200.0
82	118.0
83	36.0
84	36.0
85	36.0
86	22.0
87	8.0
88	8.0
89	8.0
90	4.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	116183.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.31119871237617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.31358516241212	56.22939672757632
2	9.151273845223395	12.502689722248522
3	3.4775596603079406	7.126688069683172
4	1.7488597132273267	4.778668135613644
5	0.9890885265730917	3.378291144143291
6	0.5833732328704987	2.391055490045876
7	0.3868155129400499	1.8496681958634225
8	0.2910566237431646	1.5905941488858095
9	0.26207695990726504	1.6112512157544563
>10	0.7849708943376258	8.045066834218431
>50	0.010079883073356348	0.40539493729719495
>100	0.0012599853841695435	0.09123537866985704
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAGGAC	40	0.005240293	14.25	3
AGCTGCT	75	2.0425094E-4	11.4	7
AGAAGAG	60	0.0058203833	11.083333	5
>>END_MODULE