##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920821_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51717
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.397122802946804	33.0	32.0	33.0	32.0	34.0
2	32.79144188564688	33.0	32.0	34.0	32.0	34.0
3	32.910203608097916	33.0	33.0	34.0	32.0	34.0
4	33.03888469942186	33.0	33.0	34.0	32.0	34.0
5	32.998839839897904	33.0	33.0	34.0	32.0	34.0
6	35.91076435214726	37.0	37.0	37.0	33.0	37.0
7	35.95200804377671	37.0	37.0	37.0	33.0	37.0
8	35.78753601330317	37.0	35.0	37.0	33.0	37.0
9	35.93010035384883	37.0	35.0	37.0	33.0	37.0
10	35.94234004292592	37.0	35.0	37.0	33.0	37.0
11	35.82096796024518	37.0	34.0	37.0	33.0	37.0
12	35.88498946187907	37.0	34.0	37.0	33.0	37.0
13	37.63982829630489	38.0	38.0	38.0	38.0	38.0
14	37.63306069570934	38.0	38.0	38.0	38.0	38.0
15	37.624456174952144	38.0	38.0	38.0	38.0	38.0
16	37.656747297793764	38.0	38.0	38.0	38.0	38.0
17	37.666163930622425	38.0	38.0	38.0	38.0	38.0
18	37.655509793684864	38.0	38.0	38.0	38.0	38.0
19	37.64626718487151	38.0	38.0	38.0	38.0	38.0
20	37.668735618848736	38.0	38.0	38.0	38.0	38.0
21	37.665931898602004	38.0	38.0	38.0	38.0	38.0
22	37.6633795463774	38.0	38.0	38.0	38.0	38.0
23	38.327687220836474	39.0	38.0	39.0	38.0	39.0
24	38.28541872111685	39.0	38.0	39.0	38.0	39.0
25	38.23392694858557	39.0	38.0	39.0	38.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	5.0
21	10.0
22	15.0
23	20.0
24	30.0
25	45.0
26	58.0
27	84.0
28	86.0
29	99.0
30	150.0
31	230.0
32	313.0
33	567.0
34	1359.0
35	6620.0
36	37152.0
37	4868.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56853645803121	17.794922365953166	12.59740510857165	28.039136067443977
2	17.421737533112903	19.22578649186921	37.492507299340645	25.85996867567724
3	19.51195931705242	21.778138716476207	28.433590502155965	30.27631146431541
4	13.784635613047936	15.807181391031962	34.74292785737765	35.66525513854245
5	15.435930158361854	34.128043003267784	33.08003171104279	17.355995127327574
6	34.07390219850339	33.45515014405321	17.39273353056055	15.078214126882845
7	28.661755322234466	29.51447299727362	21.963764332811262	19.860007347680646
8	26.70688555020593	33.16511011852969	20.32600498868844	19.801999342575943
9	25.852234274996615	15.758841386778041	19.44428331109693	38.94464102712841
10	15.629290175377536	26.434247926213818	32.869269292495694	25.067192605912947
11	35.25726550263936	20.97956184620144	23.669199682889573	20.09397296826962
12	23.79101649360945	22.451031575690777	29.874122628922787	23.88382930177698
13	28.28470328905389	20.219656979329812	25.664675058491405	25.83096467312489
14	23.491308467235143	19.334068101397996	25.62987025542858	31.54475317593828
15	24.606995765415625	27.261828799040934	22.398824371096545	25.732351064446895
16	25.173540615271573	25.057524605062163	24.260881334957556	25.5080534447087
17	24.523850958098883	25.057524605062163	25.378502233308197	25.04012220353075
18	24.210607730533482	25.535123847090897	25.53319024692074	24.72107817545488
19	24.72107817545488	24.99951659995746	25.633737455768895	24.645667768818765
20	24.85643018736586	25.051723804551695	25.045923004041228	25.045923004041228
21	24.00951331283717	24.84289498617476	25.1948102171433	25.952781483844774
22	24.475510953844964	24.50838215673763	25.285689425140667	25.73041746427674
23	24.311154939381634	24.195138929172224	25.66660865866156	25.827097472784576
24	24.608929365585787	25.295357425991455	25.22961502020612	24.86609818821664
25	24.796488582090994	24.431038149931357	25.676276659512347	25.096196608465306
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	3.0
4	3.0
5	3.0
6	3.0
7	3.0
8	3.0
9	3.0
10	15.0
11	27.0
12	27.0
13	27.0
14	53.0
15	79.0
16	79.0
17	79.0
18	164.0
19	249.0
20	249.0
21	249.0
22	401.0
23	553.0
24	553.0
25	553.0
26	831.5
27	1110.0
28	1110.0
29	1110.0
30	1528.0
31	1946.0
32	1946.0
33	1946.0
34	2479.5
35	3013.0
36	3013.0
37	3013.0
38	3790.5
39	4568.0
40	4568.0
41	4568.0
42	5194.0
43	5820.0
44	5820.0
45	5820.0
46	6550.0
47	7280.0
48	7280.0
49	7280.0
50	7340.0
51	7400.0
52	7400.0
53	7400.0
54	7266.0
55	7132.0
56	7132.0
57	7132.0
58	6247.0
59	5362.0
60	5362.0
61	5362.0
62	4536.0
63	3710.0
64	3710.0
65	3710.0
66	2897.0
67	2084.0
68	2084.0
69	2084.0
70	1505.0
71	926.0
72	926.0
73	926.0
74	624.5
75	323.0
76	323.0
77	323.0
78	213.0
79	103.0
80	103.0
81	103.0
82	60.5
83	18.0
84	18.0
85	18.0
86	13.0
87	8.0
88	8.0
89	8.0
90	4.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	51717.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.02302917802658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.5801576542652	65.91642980064583
2	8.437515690113973	12.99766034379411
3	2.784053823366973	6.433087766111724
4	1.3204799919666617	4.068294758009939
5	0.6602399959833308	2.5426842237562117
6	0.42175026359391476	1.9490689715180693
7	0.24100015062509414	1.2993793143453798
8	0.14309383943364964	0.8817216775915077
9	0.11045840236983483	0.7657056673820988
>10	0.3012501882813677	3.145967476845138
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE